Effect of Polymeric Nanoparticles with Entrapped Fish Oil or
Mupirocin on Skin Wound Healing Using a Porcine Model

J 2022

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

KOMPRDA, Tomas; Zbysek SLADEK; Monika VICENOVA; Jana SIMONOVA; Gabriela FRANKE et al.

Základní údaje

Originální název

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

Autoři

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2022, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30212 Surgery

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/22:00126528

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

cutaneous wounds; polyunsaturated fatty acids n-3; nanoparticles; poly(lactic-co-glycolic) acid; mupirocin; collagen; hydroxyproline; cyclooxygenase-2; transforming growth factor

Štítky

14110229, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 8. 2022 08:42, Mgr. Tereza Miškechová

Anotace

V originále

The utilization of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) loaded in collagen-based scaffolds for cutaneous wound healing using a porcine model is unique for the present study. Full-depth cutaneous excisions (5 x 5 cm) on the pig dorsa were treated with pure collagen scaffold (control, C), empty PLGA NPs (NP), FO, mupirocin (MUP), PLGA NPs with entrapped FO (NP/FO) and PLGA NPs with entrapped MUP (NP/MUP). The following markers were evaluated on days 0, 3, 7, 14 and 21 post-excision: collagen, hydroxyproline (HP), angiogenesis and expressions of the COX2, EGF, COL1A1, COL1A3, TGFB1, VEGFA, CCL5 and CCR5 genes. The hypothesis that NP/FO treatment is superior to FO alone and that it is comparable to NP/MUP was tested. NP/FO treatment increased HP in comparison with both FO alone and NP/MUP (day 14) but decreased (p < 0.05) angiogenesis in comparison with FO alone (day 3). NP/FO increased (p < 0.05) the expression of the CCR5 gene (day 3) and tended (p > 0.05) to increase the expressions of the EGF (day 7, day 14), TGFB1 (day 21) and CCL5 (day 7, day 21) genes as compared with NP/MUP. NP/FO can be suggested as a suitable alternative to NP/MUP in cutaneous wound treatment.

Citovat

KOMPRDA, Tomas; Zbysek SLADEK; Monika VICENOVA; Jana SIMONOVA; Gabriela FRANKE; Katarina KACVINSKA; Cristina SABLIOV; Carlos E ASTETE; Lenka LEVA; Vendula POPELKOVA; Andrej BATIK; Lucy VOJTOVA; Břetislav LIPOVÝ a Milena MATEJOVICOVA. Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2022, roč. 23, č. 14, s. 1-20. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms23147663.

@article{2213738,
   author = {Komprda, Tomas and Sladek, Zbysek and Vicenova, Monika and Simonova, Jana and Franke, Gabriela and Kacvinska, Katarina and Sabliov, Cristina and Astete, Carlos E and Leva, Lenka and Popelkova, Vendula and Batik, Andrej and Vojtova, Lucy and Lipový, Břetislav and Matejovicova, Milena},
   article_location = {Basel},
   article_number = {14},
   doi = {https://doi.org/10.3390/ijms23147663},
   keywords = {cutaneous wounds; polyunsaturated fatty acids n-3; nanoparticles; poly(lactic-co-glycolic) acid; mupirocin; collagen; hydroxyproline; cyclooxygenase-2; transforming growth factor},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model},
   url = {https://www.mdpi.com/1422-0067/23/14/7663},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 2213738
AU  - Komprda, Tomas - Sladek, Zbysek - Vicenova, Monika - Simonova, Jana - Franke, Gabriela - Kacvinska, Katarina - Sabliov, Cristina - Astete, Carlos E - Leva, Lenka - Popelkova, Vendula - Batik, Andrej - Vojtova, Lucy - Lipový, Břetislav - Matejovicova, Milena
PY  - 2022
TI  - Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 14
SP  - 1-20
EP  - 1-20
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - cutaneous wounds
KW  - polyunsaturated fatty acids n-3
KW  - nanoparticles
KW  - poly(lactic-co-glycolic) acid
KW  - mupirocin
KW  - collagen
KW  - hydroxyproline
KW  - cyclooxygenase-2
KW  - transforming growth factor
UR  - https://www.mdpi.com/1422-0067/23/14/7663
N2  - The utilization of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) loaded in collagen-based scaffolds for cutaneous wound healing using a porcine model is unique for the present study. Full-depth cutaneous excisions (5 x 5 cm) on the pig dorsa were treated with pure collagen scaffold (control, C), empty PLGA NPs (NP), FO, mupirocin (MUP), PLGA NPs with entrapped FO (NP/FO) and PLGA NPs with entrapped MUP (NP/MUP). The following markers were evaluated on days 0, 3, 7, 14 and 21 post-excision: collagen, hydroxyproline (HP), angiogenesis and expressions of the COX2, EGF, COL1A1, COL1A3, TGFB1, VEGFA, CCL5 and CCR5 genes. The hypothesis that NP/FO treatment is superior to FO alone and that it is comparable to NP/MUP was tested. NP/FO treatment increased HP in comparison with both FO alone and NP/MUP (day 14) but decreased (p < 0.05) angiogenesis in comparison with FO alone (day 3). NP/FO increased (p < 0.05) the expression of the CCR5 gene (day 3) and tended (p > 0.05) to increase the expressions of the EGF (day 7, day 14), TGFB1 (day 21) and CCL5 (day 7, day 21) genes as compared with NP/MUP. NP/FO can be suggested as a suitable alternative to NP/MUP in cutaneous wound treatment.
ER  -

KOMPRDA, Tomas; Zbysek SLADEK; Monika VICENOVA; Jana SIMONOVA; Gabriela FRANKE; Katarina KACVINSKA; Cristina SABLIOV; Carlos E ASTETE; Lenka LEVA; Vendula POPELKOVA; Andrej BATIK; Lucy VOJTOVA; Břetislav LIPOVÝ a Milena MATEJOVICOVA. Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2022, roč.~23, č.~14, s.~1-20. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms23147663.

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