Effect of Polymeric Nanoparticles with Entrapped Fish Oil or
Mupirocin on Skin Wound Healing Using a Porcine Model

J 2022

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

KOMPRDA, Tomas, Zbysek SLADEK, Monika VICENOVA, Jana SIMONOVA, Gabriela FRANKE et. al.

Základní údaje

Originální název

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

Autoři

KOMPRDA, Tomas (203 Česká republika, garant), Zbysek SLADEK (203 Česká republika), Monika VICENOVA (203 Česká republika), Jana SIMONOVA (203 Česká republika), Gabriela FRANKE (203 Česká republika), Katarina KACVINSKA (203 Česká republika), Cristina SABLIOV, Carlos E ASTETE, Lenka LEVA (203 Česká republika), Vendula POPELKOVA (203 Česká republika), Andrej BATIK (203 Česká republika), Lucy VOJTOVA (203 Česká republika), Břetislav LIPOVÝ (203 Česká republika, domácí) a Milena MATEJOVICOVA (203 Česká republika)

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2022, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30212 Surgery

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.600

Kód RIV

RIV/00216224:14110/22:00126528

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/ijms23147663

UT WoS

000832282300001

Klíčová slova anglicky

cutaneous wounds; polyunsaturated fatty acids n-3; nanoparticles; poly(lactic-co-glycolic) acid; mupirocin; collagen; hydroxyproline; cyclooxygenase-2; transforming growth factor

Štítky

14110229, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 8. 2022 08:42, Mgr. Tereza Miškechová

Anotace

V originále

The utilization of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) loaded in collagen-based scaffolds for cutaneous wound healing using a porcine model is unique for the present study. Full-depth cutaneous excisions (5 x 5 cm) on the pig dorsa were treated with pure collagen scaffold (control, C), empty PLGA NPs (NP), FO, mupirocin (MUP), PLGA NPs with entrapped FO (NP/FO) and PLGA NPs with entrapped MUP (NP/MUP). The following markers were evaluated on days 0, 3, 7, 14 and 21 post-excision: collagen, hydroxyproline (HP), angiogenesis and expressions of the COX2, EGF, COL1A1, COL1A3, TGFB1, VEGFA, CCL5 and CCR5 genes. The hypothesis that NP/FO treatment is superior to FO alone and that it is comparable to NP/MUP was tested. NP/FO treatment increased HP in comparison with both FO alone and NP/MUP (day 14) but decreased (p < 0.05) angiogenesis in comparison with FO alone (day 3). NP/FO increased (p < 0.05) the expression of the CCR5 gene (day 3) and tended (p > 0.05) to increase the expressions of the EGF (day 7, day 14), TGFB1 (day 21) and CCL5 (day 7, day 21) genes as compared with NP/MUP. NP/FO can be suggested as a suitable alternative to NP/MUP in cutaneous wound treatment.

Citovat

KOMPRDA, Tomas, Zbysek SLADEK, Monika VICENOVA, Jana SIMONOVA, Gabriela FRANKE, Katarina KACVINSKA, Cristina SABLIOV, Carlos E ASTETE, Lenka LEVA, Vendula POPELKOVA, Andrej BATIK, Lucy VOJTOVA, Břetislav LIPOVÝ a Milena MATEJOVICOVA. Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2022, roč. 23, č. 14, s. 1-20. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms23147663.

@article{2213738,
   author = {Komprda, Tomas and Sladek, Zbysek and Vicenova, Monika and Simonova, Jana and Franke, Gabriela and Kacvinska, Katarina and Sabliov, Cristina and Astete, Carlos E and Leva, Lenka and Popelkova, Vendula and Batik, Andrej and Vojtova, Lucy and Lipový, Břetislav and Matejovicova, Milena},
   article_location = {Basel},
   article_number = {14},
   doi = {http://dx.doi.org/10.3390/ijms23147663},
   keywords = {cutaneous wounds; polyunsaturated fatty acids n-3; nanoparticles; poly(lactic-co-glycolic) acid; mupirocin; collagen; hydroxyproline; cyclooxygenase-2; transforming growth factor},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model},
   url = {https://www.mdpi.com/1422-0067/23/14/7663},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 2213738
AU  - Komprda, Tomas - Sladek, Zbysek - Vicenova, Monika - Simonova, Jana - Franke, Gabriela - Kacvinska, Katarina - Sabliov, Cristina - Astete, Carlos E - Leva, Lenka - Popelkova, Vendula - Batik, Andrej - Vojtova, Lucy - Lipový, Břetislav - Matejovicova, Milena
PY  - 2022
TI  - Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 14
SP  - 1-20
EP  - 1-20
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - cutaneous wounds
KW  - polyunsaturated fatty acids n-3
KW  - nanoparticles
KW  - poly(lactic-co-glycolic) acid
KW  - mupirocin
KW  - collagen
KW  - hydroxyproline
KW  - cyclooxygenase-2
KW  - transforming growth factor
UR  - https://www.mdpi.com/1422-0067/23/14/7663
N2  - The utilization of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) loaded in collagen-based scaffolds for cutaneous wound healing using a porcine model is unique for the present study. Full-depth cutaneous excisions (5 x 5 cm) on the pig dorsa were treated with pure collagen scaffold (control, C), empty PLGA NPs (NP), FO, mupirocin (MUP), PLGA NPs with entrapped FO (NP/FO) and PLGA NPs with entrapped MUP (NP/MUP). The following markers were evaluated on days 0, 3, 7, 14 and 21 post-excision: collagen, hydroxyproline (HP), angiogenesis and expressions of the COX2, EGF, COL1A1, COL1A3, TGFB1, VEGFA, CCL5 and CCR5 genes. The hypothesis that NP/FO treatment is superior to FO alone and that it is comparable to NP/MUP was tested. NP/FO treatment increased HP in comparison with both FO alone and NP/MUP (day 14) but decreased (p < 0.05) angiogenesis in comparison with FO alone (day 3). NP/FO increased (p < 0.05) the expression of the CCR5 gene (day 3) and tended (p > 0.05) to increase the expressions of the EGF (day 7, day 14), TGFB1 (day 21) and CCL5 (day 7, day 21) genes as compared with NP/MUP. NP/FO can be suggested as a suitable alternative to NP/MUP in cutaneous wound treatment.
ER  -

KOMPRDA, Tomas, Zbysek SLADEK, Monika VICENOVA, Jana SIMONOVA, Gabriela FRANKE, Katarina KACVINSKA, Cristina SABLIOV, Carlos E ASTETE, Lenka LEVA, Vendula POPELKOVA, Andrej BATIK, Lucy VOJTOVA, Břetislav LIPOVÝ a Milena MATEJOVICOVA. Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2022, roč.~23, č.~14, s.~1-20. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms23147663.

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