Transcriptomic and proteomic profiling of peptidase expression
in Fasciola hepatica eggs developing at host's body temperature

J 2022

Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature

ILGOVÁ, Jana, Jiří VOREL, Pavel ROUDNICKÝ, Lucie ŠKORPÍKOVÁ, Martin HORN et. al.

Základní údaje

Originální název

Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature

Autoři

ILGOVÁ, Jana (703 Slovensko, garant, domácí), Jiří VOREL (203 Česká republika, domácí), Pavel ROUDNICKÝ (203 Česká republika, domácí), Lucie ŠKORPÍKOVÁ (203 Česká republika, domácí), Martin HORN (203 Česká republika) a Martin KAŠNÝ (203 Česká republika, domácí)

Vydání

SCIENTIFIC REPORTS, ENGLAND, NATURE PORTFOLIO, 2022, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600

Kód RIV

RIV/00216224:14310/22:00126553

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1038/s41598-022-14419-z

UT WoS

000838090200060

Klíčová slova anglicky

Fasciola hepatica; eggs; embryonation; transcriptomics; proteomics; RNAseq; Mass spectrometry

Štítky

CF BioData, CF GEN, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2024 08:45, Ing. Monika Szurmanová, Ph.D.

Anotace

V originále

Fasciola hepatica is a global parasite of livestock which also causes a neglected zoonosis in humans. The parasite's communication with the host during its complicated lifecycle is based on an ingenious enzymatic apparatus which includes a variety of peptidases. These enzymes are implicated in parasite migration, pathogenesis of the disease, and modification of host immune response. Although the dynamics of proteolytic machinery produced by intra-mammalian F. hepatica life stages has been previously investigated in great detail, peptidases of the eggs so far received little scientific attention. In this study, we performed a comparative RNA-seq analysis aimed at identification of peptidases expressed in F. hepatica eggs, cultured at 37 degrees C to represent gall bladder retained eggs, for different time periods and employed mass spectrometry in order to identify and quantify peptidases translated in F. hepatica egg lysates. We demonstrated that F. hepatica eggs undergo significant molecular changes when cultured at the physiological temperature of the definitive host. Egg transcriptome is subject to numerous subtle changes while their proteome is even more variable. The peptidase profile is considerably modified on both transcriptome and proteome level. Finally, we measured and classified proteolytic activities in extracts from F. hepatica eggs using a library of fluorogenic substrates and peptidase class-selective inhibitors. Activities of threonine peptidases were detected constantly, while the cysteine peptidases prevailing in freshly laid eggs are substituted by aspartic peptidase and metallopeptidase activities in the later stages of egg development.

Návaznosti

GA19-17269S, projekt VaV

Název: Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace (Akronym: Proteolýza vajec červů)

Investor: Grantová agentura ČR, Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90131, velká výzkumná infrastruktura

Název: ELIXIR-CZ II

90140, velká výzkumná infrastruktura

Název: e-INFRA CZ

Citovat

ILGOVÁ, Jana, Jiří VOREL, Pavel ROUDNICKÝ, Lucie ŠKORPÍKOVÁ, Martin HORN a Martin KAŠNÝ. Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature. SCIENTIFIC REPORTS. ENGLAND: NATURE PORTFOLIO, 2022, roč. 12, č. 1, s. 1-18. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-022-14419-z.

@article{2214302,
   author = {Ilgová, Jana and Vorel, Jiří and Roudnický, Pavel and Škorpíková, Lucie and Horn, Martin and Kašný, Martin},
   article_location = {ENGLAND},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-022-14419-z},
   keywords = {Fasciola hepatica; eggs; embryonation; transcriptomics; proteomics; RNAseq; Mass spectrometry},
   language = {eng},
   issn = {2045-2322},
   journal = {SCIENTIFIC REPORTS},
   title = {Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature},
   url = {https://doi.org/10.1038/s41598-022-14419-z},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 2214302
AU  - Ilgová, Jana - Vorel, Jiří - Roudnický, Pavel - Škorpíková, Lucie - Horn, Martin - Kašný, Martin
PY  - 2022
TI  - Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature
JF  - SCIENTIFIC REPORTS
VL  - 12
IS  - 1
SP  - 1-18
EP  - 1-18
PB  - NATURE PORTFOLIO
SN  - 20452322
KW  - Fasciola hepatica
KW  - eggs
KW  - embryonation
KW  - transcriptomics
KW  - proteomics
KW  - RNAseq
KW  - Mass spectrometry
UR  - https://doi.org/10.1038/s41598-022-14419-z
N2  - Fasciola hepatica is a global parasite of livestock which also causes a neglected zoonosis in humans. The parasite's communication with the host during its complicated lifecycle is based on an ingenious enzymatic apparatus which includes a variety of peptidases. These enzymes are implicated in parasite migration, pathogenesis of the disease, and modification of host immune response. Although the dynamics of proteolytic machinery produced by intra-mammalian F. hepatica life stages has been previously investigated in great detail, peptidases of the eggs so far received little scientific attention. In this study, we performed a comparative RNA-seq analysis aimed at identification of peptidases expressed in F. hepatica eggs, cultured at 37 degrees C to represent gall bladder retained eggs, for different time periods and employed mass spectrometry in order to identify and quantify peptidases translated in F. hepatica egg lysates. We demonstrated that F. hepatica eggs undergo significant molecular changes when cultured at the physiological temperature of the definitive host. Egg transcriptome is subject to numerous subtle changes while their proteome is even more variable. The peptidase profile is considerably modified on both transcriptome and proteome level. Finally, we measured and classified proteolytic activities in extracts from F. hepatica eggs using a library of fluorogenic substrates and peptidase class-selective inhibitors. Activities of threonine peptidases were detected constantly, while the cysteine peptidases prevailing in freshly laid eggs are substituted by aspartic peptidase and metallopeptidase activities in the later stages of egg development.
ER  -

ILGOVÁ, Jana, Jiří VOREL, Pavel ROUDNICKÝ, Lucie ŠKORPÍKOVÁ, Martin HORN a Martin KAŠNÝ. Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature. \textit{SCIENTIFIC REPORTS}. ENGLAND: NATURE PORTFOLIO, 2022, roč.~12, č.~1, s.~1-18. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-022-14419-z.

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