Omics-based approach to study honey bee (Apis mellifera) response to parasitic mite Varroa sp. and associated pathogens

DOBEŠ, Pavel, Martin KUNC, Jana HURYCHOVÁ, Sara ELIÁŠ a Pavel HYRŠL. Omics-based approach to study honey bee (Apis mellifera) response to parasitic mite Varroa sp. and associated pathogens. In Frontiers in Physiology and Disease. 2022.

Základní údaje

• Originální název: Omics-based approach to study honey bee (Apis mellifera) response to parasitic mite Varroa sp. and associated pathogens
• Název česky: Omics-based approach to study honey bee (Apis mellifera) response to parasitic mite Varroa sp. and associated pathogens
• Autoři: DOBEŠ, Pavel (203 Česká republika, garant, domácí), Martin KUNC (203 Česká republika, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Sara ELIÁŠ (276 Německo, domácí) a Pavel HYRŠL (203 Česká republika, domácí).
• Vydání: Frontiers in Physiology and Disease, 2022.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Vyžádané přednášky
• Obor: 10608 Biochemistry and molecular biology
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/22:00129442
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova česky: včela medonosná; Varroa destructor; parazitismus; transkriptomika; proteomika; metabolomika; imunitní odpověď; přezimování
• Klíčová slova anglicky: honey bee; Varroa destructor; parasitism; transcriptomics; proteomics; metabolomics; immune response; overwintering
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Honey bees (Apis mellifera L.) are the most important managed pollinators worldwide, and according to the estimates, about two-thirds of crops used in human food production are dependent on pollination. A long history of domestication and international transport of A. mellifera has resulted in a cosmopolitan distribution of the bees, but unfortunately, also its pathogens and parasites, which are considered to be one of the main factors behind honey bee losses. Therefore, the global decline of the honey bee population poses a significant social, economic, and scientific concern. Despite years of intensive research, the complex mechanisms of Varroa-honey bee interaction are still not untangled. Therefore, we employed a unique combination of transcriptomic, proteomic, metabolomic, and functional analyses to reveal new details about the effect of Varroa mites and naturally associated factors, including viruses, on honey bees. We focused on differences between Varroa-parasitised and unparasitised ten-day-old worker bees collected before overwintering from the same set of colonies reared without anti-mite treatment. Significant changes were observed at all organismal levels in immunity, oxidative stress response, olfactory recognition and other specific processes. Moreover, the supplementary comparison to honey bees collected from colonies with standard anti-Varroa treatment provides insights into the effect of a pyrethroid flumethrin. Recent findings of omics analyses help reveal new details of honey bee response to parasitic mites and suggest new ways to control diseases of this beneficial insect. Our work is supported by the Ministry of Agriculture of the Czech Republic grant NAZV no. QK1910286; SFI Research Infrastructure Call 2012 (12/RI/2346 (3)) and Projects of Large Research, Development and Innovations Infrastructures "e-Infrastruktura CZ" (e-INFRA LM2018140).

Anotace česky

Honey bees (Apis mellifera L.) are the most important managed pollinators worldwide, and according to the estimates, about two-thirds of crops used in human food production are dependent on pollination. A long history of domestication and international transport of A. mellifera has resulted in a cosmopolitan distribution of the bees, but unfortunately, also its pathogens and parasites, which are considered to be one of the main factors behind honey bee losses. Therefore, the global decline of the honey bee population poses a significant social, economic, and scientific concern. Despite years of intensive research, the complex mechanisms of Varroa-honey bee interaction are still not untangled. Therefore, we employed a unique combination of transcriptomic, proteomic, metabolomic, and functional analyses to reveal new details about the effect of Varroa mites and naturally associated factors, including viruses, on honey bees. We focused on differences between Varroa-parasitised and unparasitised ten-day-old worker bees collected before overwintering from the same set of colonies reared without anti-mite treatment. Significant changes were observed at all organismal levels in immunity, oxidative stress response, olfactory recognition and other specific processes. Moreover, the supplementary comparison to honey bees collected from colonies with standard anti-Varroa treatment provides insights into the effect of a pyrethroid flumethrin. Recent findings of omics analyses help reveal new details of honey bee response to parasitic mites and suggest new ways to control diseases of this beneficial insect. Our work is supported by the Ministry of Agriculture of the Czech Republic grant NAZV no. QK1910286; SFI Research Infrastructure Call 2012 (12/RI/2346 (3)) and Projects of Large Research, Development and Innovations Infrastructures "e-Infrastruktura CZ" (e-INFRA LM2018140).

Návaznosti

• QK1910286, projekt VaV: Název: Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

Investor: Ministerstvo zemědělství ČR, Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev