Compatible interaction of Brachypodium distachyon and
endophytic fungus Microdochium bolleyi

J 2022

Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi

MATUŠINSKY, Pavel; Božena SEDLÁKOVÁ a Dominik BLEŠA

Základní údaje

Originální název

Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi

Autoři

MATUŠINSKY, Pavel; Božena SEDLÁKOVÁ a Dominik BLEŠA

Vydání

PLoS ONE, San Francisco, Public Library of Science, 2022, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40106 Agronomy, plant breeding and plant protection

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00126702

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Wheat; Fungi; Plant fungal pathogens; Leaves; Gene expression; Polymerase chain reaction; Fusarium; Pathogens

Štítky

14314010, 14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 1. 2023 11:18, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Brachypodium distachyon is a useful model organism for studying interaction of cereals with phytopathogenic fungi. The present study tested the possibility of a compatible interaction of B. distachyon with the endophytic fungus Microdochium bolleyi originated from wheat roots. There was evaluated the effect of this endophytic fungus on the intensity of the attack by pathogen Fusarium culmorum in B. distachyon and wheat, and also changes in expression of genes (in B. distachyon: BdChitinase1, BdPR1-5, BdLOX3, BdPAL, BdEIN3, and BdAOS; and in wheat: TaB2H2(chitinase), TaPR1.1, TaLOX, TaPAL, TaEIN2, and TaAOS) involved in defence against pathogens. Using light microscopy and newly developed specific primers was found to be root colonization of B. distachyon by the endophyte M. bolleyi. B. distachyon plants, as well as wheat inoculated with M. bolleyi showed significantly weaker symptoms on leaves from infection by fungus F. culmorum than did plants without the endophyte. Expression of genes BdPR1-5, BdChitinase1, and BdLOX3 in B. distachyon and of TaPR1.1 and TaB2H2 in wheat was upregulated after infection with F. culmorum. M. bolleyi-mediated resistance in B. distachyon was independent of the expression of the most tested genes. Taken together, the results of the present study show that B. distachyon can be used as a model host system for endophytic fungus M. bolleyi.

Citovat

MATUŠINSKY, Pavel; Božena SEDLÁKOVÁ a Dominik BLEŠA. Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi. PLoS ONE. San Francisco: Public Library of Science, 2022, roč. 17, č. 3, s. 1-19. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0265357.

@article{2218797,
   author = {Matušinsky, Pavel and Sedláková, Božena and Bleša, Dominik},
   article_location = {San Francisco},
   article_number = {3},
   doi = {https://doi.org/10.1371/journal.pone.0265357},
   keywords = {Wheat; Fungi; Plant fungal pathogens; Leaves; Gene expression; Polymerase chain reaction; Fusarium; Pathogens},
   language = {eng},
   issn = {1932-6203},
   journal = {PLoS ONE},
   title = {Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi},
   url = {https://doi.org/10.1371/journal.pone.0265357},
   volume = {17},
   year = {2022}
}

TY  - JOUR
ID  - 2218797
AU  - Matušinsky, Pavel - Sedláková, Božena - Bleša, Dominik
PY  - 2022
TI  - Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi
JF  - PLoS ONE
VL  - 17
IS  - 3
SP  - 1-19
EP  - 1-19
PB  - Public Library of Science
SN  - 19326203
KW  - Wheat
KW  - Fungi
KW  - Plant fungal pathogens
KW  - Leaves
KW  - Gene expression
KW  - Polymerase chain reaction
KW  - Fusarium
KW  - Pathogens
UR  - https://doi.org/10.1371/journal.pone.0265357
N2  - Brachypodium distachyon is a useful model organism for studying interaction of cereals with phytopathogenic fungi. The present study tested the possibility of a compatible interaction of B. distachyon with the endophytic fungus Microdochium bolleyi originated from wheat roots. There was evaluated the effect of this endophytic fungus on the intensity of the attack by pathogen Fusarium culmorum in B. distachyon and wheat, and also changes in expression of genes (in B. distachyon: BdChitinase1, BdPR1-5, BdLOX3, BdPAL, BdEIN3, and BdAOS; and in wheat: TaB2H2(chitinase), TaPR1.1, TaLOX, TaPAL, TaEIN2, and TaAOS) involved in defence against pathogens. Using light microscopy and newly developed specific primers was found to be root colonization of B. distachyon by the endophyte M. bolleyi. B. distachyon plants, as well as wheat inoculated with M. bolleyi showed significantly weaker symptoms on leaves from infection by fungus F. culmorum than did plants without the endophyte. Expression of genes BdPR1-5, BdChitinase1, and BdLOX3 in B. distachyon and of TaPR1.1 and TaB2H2 in wheat was upregulated after infection with F. culmorum. M. bolleyi-mediated resistance in B. distachyon was independent of the expression of the most tested genes. Taken together, the results of the present study show that B. distachyon can be used as a model host system for endophytic fungus M. bolleyi.
ER  -

MATUŠINSKY, Pavel; Božena SEDLÁKOVÁ a Dominik BLEŠA. Compatible interaction of Brachypodium distachyon and endophytic fungus Microdochium bolleyi. \textit{PLoS ONE}. San Francisco: Public Library of Science, 2022, roč.~17, č.~3, s.~1-19. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0265357.

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