Kinetochore size scales with chromosome size in bimodal
karyotypes of Agavoideae

J 2022

Kinetochore size scales with chromosome size in bimodal karyotypes of Agavoideae

PLAČKOVÁ, Klára; František ZEDEK; Veit SCHUBERT; Andreas HOUBEN; Petr BUREŠ et al.

Základní údaje

Originální název

Kinetochore size scales with chromosome size in bimodal karyotypes of Agavoideae

Autoři

PLAČKOVÁ, Klára; František ZEDEK; Veit SCHUBERT; Andreas HOUBEN a Petr BUREŠ

Vydání

Annals of Botany, Oxford, Oxford University Press, 2022, 0305-7364

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00129165

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Asparagaceae; cell division; centromere; chromosome size evolution; genome size evolution; intracellular scaling; linear mixed models; structured illumination microscopy

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 1. 2024 00:24, prof. RNDr. Petr Bureš, Ph.D.

Anotace

V originále

Background and Aims In eukaryotes, the total kinetochore size (defined as a chromosomal region containing CENH3-positive nucleosomes) per nucleus strongly correlates with genome size, a relationship that has been hypothesized to stem from general intracellular scaling principles. However, if larger chromosomes within a karyotype required larger kinetochores to move properly, it could also be derived from the mechanics of cell division. Methods We selected seven species of the plant subfamily Agavoideae whose karyotypes are characterized by the presence of small and very large chromosomes. We visualized the kinetochore regions and chromosomes by immunolabelling with an anti-CENH3 antibody and DAPI (6 '-diamidino-2-phenylindole) staining. We then employed 2D widefield and 3D super-resolution microscopy to measure chromosome and kinetochore areas and volumes, respectively. To assess the scaling relationship of kinetochore size to chromosome size inside a karyotype, we log-transformed the data and analysed them with linear mixed models which allowed us to control for the inherent hierarchical structure of the dataset (metaphases within slides and species). Key Results We found a positive intra-karyotype relationship between kinetochore and chromosome size. The slope of the regression line of the observed relationship (0.277 for areas, 0.247 for volumes) was very close to the theoretical slope of 0.25 for chromosome width based on the expected physics of chromosome passage through the cytoplasm during cell division. We obtained similar results by reanalysing available data from human and maize. Conclusions Our findings suggest that the total kinetochore size to genome size scaling observed across eukaryotes may also originate from the mechanics of cell division. Moreover, the potential causal link between kinetochore and chromosome size indicates that evolutionary mechanisms capable of leading kinetochore size changes to fixation, such as centromere drive, could promote the size evolution of entire chromosomes and genomes.

Návaznosti

GA20-15989S, projekt VaV

Název: Evoluce velikosti genomu - centromerický drajv v nové roli (Akronym: Centrogenomtah)

Investor: Grantová agentura ČR, Evolution of genome size - a new role for the centromere drive

MUNI/C/1660/2019, interní kód MU

Název: Velikost kinetochoru ve vztahu k velikosti chromosomů

Investor: Masarykova univerzita, Velikost kinetochoru ve vztahu k velikosti chromosomů, DO R. 2020 - Program rektora

Citovat

PLAČKOVÁ, Klára; František ZEDEK; Veit SCHUBERT; Andreas HOUBEN a Petr BUREŠ. Kinetochore size scales with chromosome size in bimodal karyotypes of Agavoideae. Annals of Botany. Oxford: Oxford University Press, 2022, roč. 130, č. 1, s. 77-84. ISSN 0305-7364. Dostupné z: https://doi.org/10.1093/aob/mcac063.

@article{2219018,
   author = {Plačková, Klára and Zedek, František and Schubert, Veit and Houben, Andreas and Bureš, Petr},
   article_location = {Oxford},
   article_number = {1},
   doi = {https://doi.org/10.1093/aob/mcac063},
   keywords = {Asparagaceae; cell division; centromere; chromosome size evolution; genome size evolution; intracellular scaling; linear mixed models; structured illumination microscopy},
   language = {eng},
   issn = {0305-7364},
   journal = {Annals of Botany},
   title = {Kinetochore size scales with chromosome size in bimodal karyotypes of Agavoideae},
   url = {https://doi.org/10.1093/aob/mcac063},
   volume = {130},
   year = {2022}
}

TY  - JOUR
ID  - 2219018
AU  - Plačková, Klára - Zedek, František - Schubert, Veit - Houben, Andreas - Bureš, Petr
PY  - 2022
TI  - Kinetochore size scales with chromosome size in bimodal karyotypes of Agavoideae
JF  - Annals of Botany
VL  - 130
IS  - 1
SP  - 77-84
EP  - 77-84
PB  - Oxford University Press
SN  - 03057364
KW  - Asparagaceae
KW  - cell division
KW  - centromere
KW  - chromosome size evolution
KW  - genome size evolution
KW  - intracellular scaling
KW  - linear mixed models
KW  - structured illumination microscopy
UR  - https://doi.org/10.1093/aob/mcac063
N2  - Background and Aims In eukaryotes, the total kinetochore size (defined as a chromosomal region containing CENH3-positive nucleosomes) per nucleus strongly correlates with genome size, a relationship that has been hypothesized to stem from general intracellular scaling principles. However, if larger chromosomes within a karyotype required larger kinetochores to move properly, it could also be derived from the mechanics of cell division. Methods We selected seven species of the plant subfamily Agavoideae whose karyotypes are characterized by the presence of small and very large chromosomes. We visualized the kinetochore regions and chromosomes by immunolabelling with an anti-CENH3 antibody and DAPI (6 '-diamidino-2-phenylindole) staining. We then employed 2D widefield and 3D super-resolution microscopy to measure chromosome and kinetochore areas and volumes, respectively. To assess the scaling relationship of kinetochore size to chromosome size inside a karyotype, we log-transformed the data and analysed them with linear mixed models which allowed us to control for the inherent hierarchical structure of the dataset (metaphases within slides and species). Key Results We found a positive intra-karyotype relationship between kinetochore and chromosome size. The slope of the regression line of the observed relationship (0.277 for areas, 0.247 for volumes) was very close to the theoretical slope of 0.25 for chromosome width based on the expected physics of chromosome passage through the cytoplasm during cell division. We obtained similar results by reanalysing available data from human and maize. Conclusions Our findings suggest that the total kinetochore size to genome size scaling observed across eukaryotes may also originate from the mechanics of cell division. Moreover, the potential causal link between kinetochore and chromosome size indicates that evolutionary mechanisms capable of leading kinetochore size changes to fixation, such as centromere drive, could promote the size evolution of entire chromosomes and genomes.
ER  -

PLAČKOVÁ, Klára; František ZEDEK; Veit SCHUBERT; Andreas HOUBEN a Petr BUREŠ. Kinetochore size scales with chromosome size in bimodal karyotypes of Agavoideae. \textit{Annals of Botany}. Oxford: Oxford University Press, 2022, roč.~130, č.~1, s.~77-84. ISSN~0305-7364. Dostupné z: https://doi.org/10.1093/aob/mcac063.

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