Molecular Research of Lipid Peroxidation and Antioxidant Enzyme
Activity of Comamonas testosteroni Bacterial Cells under the
Hexachlorobenzene Impact

J 2022

Molecular Research of Lipid Peroxidation and Antioxidant Enzyme Activity of Comamonas testosteroni Bacterial Cells under the Hexachlorobenzene Impact

DIMOVA, Mariia; Andrii TUGAI; Tetiana TUGAI; Galyna IUTYNSKA; Dani DORDEVIC et al.

Základní údaje

Originální název

Molecular Research of Lipid Peroxidation and Antioxidant Enzyme Activity of Comamonas testosteroni Bacterial Cells under the Hexachlorobenzene Impact

Autoři

DIMOVA, Mariia; Andrii TUGAI; Tetiana TUGAI; Galyna IUTYNSKA; Dani DORDEVIC a Ivan KUSHKEVYCH

Vydání

International Journal of Molecular Sciences, MDPI, 2022, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00126785

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

diene and triene conjugates; Schiff bases; catalase; peroxidase; malondialdehyde; hexachlorobenzene

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 1. 2023 15:20, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The species of Comamonas testosteroni is the most common human pathogen of the genus, which can be associated with acute appendicitis, infections of the bloodstream, the peritoneal cavity, cerebrospinal fluid, inflammatory bowel disease, and in general, bacteremia. According to the literature, Comamonas testosteroni has destructive activity to a wide range of toxic chemical compounds, including chlorobenzenes. The specified strains were isolated from the soil of the organochlorine waste landfill, where hexachlorobenzene (HCB) was predominant. These strains were expected to be capable of degrading HCB. Microbiological (bacterial enrichment and cultivating, bacterial biomass obtaining), molecular biology, biochemical (enzymatic activities, malondialdehyde measuring, peroxidation lipid products measuring), and statistical methods were carried out in this research. The reaction of both strains (UCM B-400 and UCM B-401) to the hexachlorobenzene presence differed in the content of diene and triene conjugates and malondialdehyde, as well as different catalase and peroxidase activity levels. In terms of primary peroxidation products, diene conjugates were lower, except conditions with 20 mg/L HCB, where these were higher up to two times, than the pure control. Malondialdehyde in strain B-400 cells decreased up to five times, in B-401, but increased up to two times, compared to the pure control. Schiff bases in strain B-400 cells were 2–3 times lower than the pure control. However, in B-401 cells Schiff bases under higher HCB dose were in the same level with the pure control. Catalase activity was 1.5 times higher in all experimental variants, compared to the pure control (in the strain B-401 cells), but in the B-400 strain, cells were 2 times lower, compared to the pure control. The response of the two strains to hexachlorobenzene was similar only in peroxidase activity terms, which was slightly higher compared to the pure control. The physiological response of Comamonas testosteroni strains to hexachlorobenzene has a typical strain reaction. The physiological response level of these strains to hexachlorobenzene confirms its tolerance, and indirectly, the ability to destroy the specified toxic compound.

Návaznosti

MUNI/A/1221/2021, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie 2 (Akronym: SVMIK)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie 2

Citovat

DIMOVA, Mariia; Andrii TUGAI; Tetiana TUGAI; Galyna IUTYNSKA; Dani DORDEVIC a Ivan KUSHKEVYCH. Molecular Research of Lipid Peroxidation and Antioxidant Enzyme Activity of Comamonas testosteroni Bacterial Cells under the Hexachlorobenzene Impact. International Journal of Molecular Sciences. MDPI, 2022, roč. 23, č. 19, s. 1-11. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms231911415.

@article{2221483,
   author = {Dimova, Mariia and Tugai, Andrii and Tugai, Tetiana and Iutynska, Galyna and Dordevic, Dani and Kushkevych, Ivan},
   article_number = {19},
   doi = {https://doi.org/10.3390/ijms231911415},
   keywords = {diene and triene conjugates; Schiff bases; catalase; peroxidase; malondialdehyde; hexachlorobenzene},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Molecular Research of Lipid Peroxidation and Antioxidant Enzyme Activity of Comamonas testosteroni Bacterial Cells under the Hexachlorobenzene Impact},
   url = {https://www.mdpi.com/1422-0067/23/19/11415},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 2221483
AU  - Dimova, Mariia - Tugai, Andrii - Tugai, Tetiana - Iutynska, Galyna - Dordevic, Dani - Kushkevych, Ivan
PY  - 2022
TI  - Molecular Research of Lipid Peroxidation and Antioxidant Enzyme Activity of Comamonas testosteroni Bacterial Cells under the Hexachlorobenzene Impact
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 19
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 14220067
KW  - diene and triene conjugates
KW  - Schiff bases
KW  - catalase
KW  - peroxidase
KW  - malondialdehyde
KW  - hexachlorobenzene
UR  - https://www.mdpi.com/1422-0067/23/19/11415
N2  - The species of Comamonas testosteroni is the most common human pathogen of the genus, which can be associated with acute appendicitis, infections of the bloodstream, the peritoneal cavity, cerebrospinal fluid, inflammatory bowel disease, and in general, bacteremia. According to the literature, Comamonas testosteroni has destructive activity to a wide range of toxic chemical compounds, including chlorobenzenes. The specified strains were isolated from the soil of the organochlorine waste landfill, where hexachlorobenzene (HCB) was predominant. These strains were expected to be capable of degrading HCB. Microbiological (bacterial enrichment and cultivating, bacterial biomass obtaining), molecular biology, biochemical (enzymatic activities, malondialdehyde measuring, peroxidation lipid products measuring), and statistical methods were carried out in this research. The reaction of both strains (UCM B-400 and UCM B-401) to the hexachlorobenzene presence differed in the content of diene and triene conjugates and malondialdehyde, as well as different catalase and peroxidase activity levels. In terms of primary peroxidation products, diene conjugates were lower, except conditions with 20 mg/L HCB, where these were higher up to two times, than the pure control. Malondialdehyde in strain B-400 cells decreased up to five times, in B-401, but increased up to two times, compared to the pure control. Schiff bases in strain B-400 cells were 2–3 times lower than the pure control. However, in B-401 cells Schiff bases under higher HCB dose were in the same level with the pure control. Catalase activity was 1.5 times higher in all experimental variants, compared to the pure control (in the strain B-401 cells), but in the B-400 strain, cells were 2 times lower, compared to the pure control. The response of the two strains to hexachlorobenzene was similar only in peroxidase activity terms, which was slightly higher compared to the pure control. The physiological response of Comamonas testosteroni strains to hexachlorobenzene has a typical strain reaction. The physiological response level of these strains to hexachlorobenzene confirms its tolerance, and indirectly, the ability to destroy the specified toxic compound.
ER  -

DIMOVA, Mariia; Andrii TUGAI; Tetiana TUGAI; Galyna IUTYNSKA; Dani DORDEVIC a Ivan KUSHKEVYCH. Molecular Research of Lipid Peroxidation and Antioxidant Enzyme Activity of Comamonas testosteroni Bacterial Cells under the Hexachlorobenzene Impact. \textit{International Journal of Molecular Sciences}. MDPI, 2022, roč.~23, č.~19, s.~1-11. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms231911415.

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