Is There a Relationship between Biofilm Forming-Capacity and
Antibiotic Resistance in Staphylococcus spp.? In Vitro Results

a 2022

Is There a Relationship between Biofilm Forming-Capacity and Antibiotic Resistance in Staphylococcus spp.? In Vitro Results

DONADU, Matthew Gavino; Marco FERRARI; Vittorio MAZZARELLO; Stefania ZANETTI; Ivan KUSHKEVYCH et al.

Základní údaje

Originální název

Is There a Relationship between Biofilm Forming-Capacity and Antibiotic Resistance in Staphylococcus spp.? In Vitro Results

Autoři

Vydání

The 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action And Mechanisms of Resistance, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISSN

DOI

https://doi.org/10.3390/eca2022-12734

Klíčová slova anglicky

Congo red agar; crystal violet; microtiter plate assay; biofilm-formation; Staphylococcus aureus; non-aureus staphylococci; methicillin-resistance; multidrug resistance; MDR; phenotypic assay

Změněno: 30. 9. 2022 09:38, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Staphylococcus species are considered important members of the normal skin microbiota, in addition to being common pathogens in human and animal infections. In addition to S. aureus, other members of the genus are now widely-recognized as pathogens, especially in immunocompromised individuals. One of the most important virulence factors of staphylococci is the formation of biofilm (slime), which enhances their survival on inanimate surfaces, in addition to providing protection against immune cells and antibiotics in vivo. There has been considerable interest in the study of the relationship between biofilm formation and the antibiotic resistant phenotype, however, the results in the available literature are inconsistent. Thus, this study aims to investigate the correlation between biofilm formation and antibiotic resistance in Staphylococcus spp. isolates using phenotypic methods. One hundred and eighty (n = 180) isolates were included in the study, with S. epidermidis (40.0%) and S. lugdunensis (10.0%) being the most numerous. Biofilm-forming capacity was assessed by means of the crystal violet microtiter-plate-based (CV-MTP) method. Methicillin-resistance (MR) was identified in 47.2% (n = 85) of isolates. Among the commonly-used antimicrobials, resistance was highest for clindamycin (51.1%), erythromycin (48.9%) and trimethoprim-sulfamethoxazole (51.1%). Based on the CV-MTP method, n = 13 (7.2%), n = 13 (7.2%), n = 42 (23.3%) and n = 113 (62.3%) staphylococcal isolates were non-biofilm-producing, weak, moderate and strong biofilm producers, respectively. No significant differences in biofilm-formation were shown to be observed on the basis of MR (susceptible (S): 0.881 ± 0.309 vs. resistant (R): 0.890 ± 0.347; p = 0.133) and according to the resistance, to most other antibiotics. Rifampin-resistant isolates were more potent biofilm-producers than their susceptible counterparts (S: 0.802 ± 0.296 vs. R: 1.194 ± 0.221; p = 0.024). The association of the antibiotic-resistant phenotype and biofilm-formation is still inconclusive, due to the heterogeneity of the results in presently available studies; however, the understanding of these mechanisms in Staphylococcus spp. is crucial to appropriately address the therapy and eradication of these pathogens.

Citovat

DONADU, Matthew Gavino; Marco FERRARI; Vittorio MAZZARELLO; Stefania ZANETTI; Ivan KUSHKEVYCH; Simon K.-M. R. RITTMANN; Anette STÁJER; Boglárka ÓNODI; Zoltán BARÁTH; Edit URBÁN a Márió GAJDÁCS. Is There a Relationship between Biofilm Forming-Capacity and Antibiotic Resistance in Staphylococcus spp.? In Vitro Results. In The 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action And Mechanisms of Resistance. 2022. ISSN 2673-9992. Dostupné z: https://doi.org/10.3390/eca2022-12734.

@proceedings{2221487,
   author = {Donadu, Matthew Gavino and Ferrari, Marco and Mazzarello, Vittorio and Zanetti, Stefania and Kushkevych, Ivan and Rittmann, Simon K.andM. R. and Stájer, Anette and Ónodi, Boglárka and Baráth, Zoltán and Urbán, Edit and Gajdács, Márió},
   booktitle = {The 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action And Mechanisms of Resistance},
   doi = {https://doi.org/10.3390/eca2022-12734},
   keywords = {Congo red agar; crystal violet; microtiter plate assay; biofilm-formation; Staphylococcus aureus; non-aureus staphylococci; methicillin-resistance; multidrug resistance; MDR; phenotypic assay},
   language = {eng},
   title = {Is There a Relationship between Biofilm Forming-Capacity and Antibiotic Resistance in Staphylococcus spp.? In Vitro Results},
   url = {https://www.mdpi.com/2673-9992/12/1/29},
   year = {2022}
}

TY  - CONF
ID  - 2221487
AU  - Donadu, Matthew Gavino - Ferrari, Marco - Mazzarello, Vittorio - Zanetti, Stefania - Kushkevych, Ivan - Rittmann, Simon K.-M. R. - Stájer, Anette - Ónodi, Boglárka - Baráth, Zoltán - Urbán, Edit - Gajdács, Márió
PY  - 2022
TI  - Is There a Relationship between Biofilm Forming-Capacity and Antibiotic Resistance in Staphylococcus spp.? In Vitro Results
KW  - Congo red agar
KW  - crystal violet
KW  - microtiter plate assay
KW  - biofilm-formation
KW  - Staphylococcus aureus
KW  - non-aureus staphylococci
KW  - methicillin-resistance
KW  - multidrug resistance
KW  - MDR
KW  - phenotypic assay
UR  - https://www.mdpi.com/2673-9992/12/1/29
N2  - Staphylococcus species are considered important members of the normal skin microbiota, in addition to being common pathogens in human and animal infections. In addition to S. aureus, other members of the genus are now widely-recognized as pathogens, especially in immunocompromised individuals. One of the most important virulence factors of staphylococci is the formation of biofilm (slime), which enhances their survival on inanimate surfaces, in addition to providing protection against immune cells and antibiotics in vivo. There has been considerable interest in the study of the relationship between biofilm formation and the antibiotic resistant phenotype, however, the results in the available literature are inconsistent. Thus, this study aims to investigate the correlation between biofilm formation and antibiotic resistance in Staphylococcus spp. isolates using phenotypic methods. One hundred and eighty (n = 180) isolates were included in the study, with S. epidermidis (40.0%) and S. lugdunensis (10.0%) being the most numerous. Biofilm-forming capacity was assessed by means of the crystal violet microtiter-plate-based (CV-MTP) method. Methicillin-resistance (MR) was identified in 47.2% (n = 85) of isolates. Among the commonly-used antimicrobials, resistance was highest for clindamycin (51.1%), erythromycin (48.9%) and trimethoprim-sulfamethoxazole (51.1%). Based on the CV-MTP method, n = 13 (7.2%), n = 13 (7.2%), n = 42 (23.3%) and n = 113 (62.3%) staphylococcal isolates were non-biofilm-producing, weak, moderate and strong biofilm producers, respectively. No significant differences in biofilm-formation were shown to be observed on the basis of MR (susceptible (S): 0.881 ± 0.309 vs. resistant (R): 0.890 ± 0.347; p = 0.133) and according to the resistance, to most other antibiotics. Rifampin-resistant isolates were more potent biofilm-producers than their susceptible counterparts (S: 0.802 ± 0.296 vs. R: 1.194 ± 0.221; p = 0.024). The association of the antibiotic-resistant phenotype and biofilm-formation is still inconclusive, due to the heterogeneity of the results in presently available studies; however, the understanding of these mechanisms in Staphylococcus spp. is crucial to appropriately address the therapy and eradication of these pathogens.
ER  -

DONADU, Matthew Gavino; Marco FERRARI; Vittorio MAZZARELLO; Stefania ZANETTI; Ivan KUSHKEVYCH; Simon K.-M. R. RITTMANN; Anette STÁJER; Boglárka ÓNODI; Zoltán BARÁTH; Edit URBÁN a Márió GAJDÁCS. Is There a Relationship between Biofilm Forming-Capacity and Antibiotic Resistance in Staphylococcus spp.? In Vitro Results. In \textit{The 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action And Mechanisms of Resistance}. 2022. ISSN~2673-9992. Dostupné z: https://doi.org/10.3390/eca2022-12734.

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