Performance of bat-derived macrophages at different
temperatures

J 2022

Performance of bat-derived macrophages at different temperatures

NEMCOVA, Monika, Veronika SEIDLOVA, Jan ZUKAL, Heliana DUNDAROVA, Katerina ZUKALOVA et. al.

Základní údaje

Originální název

Performance of bat-derived macrophages at different temperatures

Autoři

NEMCOVA, Monika, Veronika SEIDLOVA, Jan ZUKAL (203 Česká republika, domácí), Heliana DUNDAROVA, Katerina ZUKALOVA a Jiri PIKULA (garant)

Vydání

FRONTIERS IN VETERINARY SCIENCE, SWITZERLAND, FRONTIERS MEDIA SA, 2022, 2297-1769

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.200

Kód RIV

RIV/00216224:14310/22:00126822

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3389/fvets.2022.978756

UT WoS

000858548200001

Klíčová slova anglicky

Chiroptera (bats)in vitro modelhibernationphagocytic activitytemperature-dependent proliferationdaily torpormacrophage biology

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 12. 2022 11:40, Mgr. Marie Šípková, DiS.

Anotace

V originále

Heterothermy, as a temperature-dependent physiological continuum, may affect host-pathogen interactions through modulation of immune responses. Here, we evaluated proliferation and functional performance of a macrophage cell line established from the greater mouse-eared (Myotis myotis) bat at 8, 17.5, and 37 degrees C to simulate body temperatures during hibernation, daily torpor and euthermia. Macrophages were also frozen to -20 degrees C and then examined for their ability to proliferate in the immediate post-thaw period. We show that bat macrophages can proliferate at lower temperatures, though their growth rate is significantly slower than at 37 degrees C. The cells differed in their shape, size and ability to attach to the plate surface at both lower temperatures, being spheroidal and free in suspension at 8 degrees C and epithelial-like, spindle-shaped and/or spheroidal at 17.5 degrees C. While phagocytosis at temperatures of 8 and 17.5 degrees C amounted to 85.8 and 83.1% of the activity observed at 37 degrees C, respectively, full phagocytic activity was restored within minutes of translocation into a higher temperature. Bat-derived macrophages were also able to withstand temperatures of -20 degrees C in a cryoprotectant-free cultivation medium and, in the immediate post-thaw period, became viable and were able to proliferate. Our in vitro data enhance understanding of macrophage biology.

Citovat

NEMCOVA, Monika, Veronika SEIDLOVA, Jan ZUKAL, Heliana DUNDAROVA, Katerina ZUKALOVA a Jiri PIKULA. Performance of bat-derived macrophages at different temperatures. FRONTIERS IN VETERINARY SCIENCE. SWITZERLAND: FRONTIERS MEDIA SA, 2022, roč. 9, Sep, s. 1-12. ISSN 2297-1769. Dostupné z: https://dx.doi.org/10.3389/fvets.2022.978756.

@article{2222890,
   author = {Nemcova, Monika and Seidlova, Veronika and Zukal, Jan and Dundarova, Heliana and Zukalova, Katerina and Pikula, Jiri},
   article_location = {SWITZERLAND},
   article_number = {Sep},
   doi = {http://dx.doi.org/10.3389/fvets.2022.978756},
   keywords = {Chiroptera (bats)in vitro modelhibernationphagocytic activitytemperature-dependent proliferationdaily torpormacrophage biology},
   language = {eng},
   issn = {2297-1769},
   journal = {FRONTIERS IN VETERINARY SCIENCE},
   title = {Performance of bat-derived macrophages at different temperatures},
   url = {https://doi.org/10.3389/fvets.2022.978756},
   volume = {9},
   year = {2022}
}

TY  - JOUR
ID  - 2222890
AU  - Nemcova, Monika - Seidlova, Veronika - Zukal, Jan - Dundarova, Heliana - Zukalova, Katerina - Pikula, Jiri
PY  - 2022
TI  - Performance of bat-derived macrophages at different temperatures
JF  - FRONTIERS IN VETERINARY SCIENCE
VL  - 9
IS  - Sep
SP  - 1-12
EP  - 1-12
PB  - FRONTIERS MEDIA SA
SN  - 22971769
KW  - Chiroptera (bats)in vitro modelhibernationphagocytic activitytemperature-dependent proliferationdaily torpormacrophage biology
UR  - https://doi.org/10.3389/fvets.2022.978756
N2  - Heterothermy, as a temperature-dependent physiological continuum, may affect host-pathogen interactions through modulation of immune responses. Here, we evaluated proliferation and functional performance of a macrophage cell line established from the greater mouse-eared (Myotis myotis) bat at 8, 17.5, and 37 degrees C to simulate body temperatures during hibernation, daily torpor and euthermia. Macrophages were also frozen to -20 degrees C and then examined for their ability to proliferate in the immediate post-thaw period. We show that bat macrophages can proliferate at lower temperatures, though their growth rate is significantly slower than at 37 degrees C. The cells differed in their shape, size and ability to attach to the plate surface at both lower temperatures, being spheroidal and free in suspension at 8 degrees C and epithelial-like, spindle-shaped and/or spheroidal at 17.5 degrees C. While phagocytosis at temperatures of 8 and 17.5 degrees C amounted to 85.8 and 83.1% of the activity observed at 37 degrees C, respectively, full phagocytic activity was restored within minutes of translocation into a higher temperature. Bat-derived macrophages were also able to withstand temperatures of -20 degrees C in a cryoprotectant-free cultivation medium and, in the immediate post-thaw period, became viable and were able to proliferate. Our in vitro data enhance understanding of macrophage biology.
ER  -

NEMCOVA, Monika, Veronika SEIDLOVA, Jan ZUKAL, Heliana DUNDAROVA, Katerina ZUKALOVA a Jiri PIKULA. Performance of bat-derived macrophages at different temperatures. \textit{FRONTIERS IN VETERINARY SCIENCE}. SWITZERLAND: FRONTIERS MEDIA SA, 2022, roč.~9, Sep, s.~1-12. ISSN~2297-1769. Dostupné z: https://dx.doi.org/10.3389/fvets.2022.978756.

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