Mega-sized pericentromeric blocks of simple telomeric repeats
and their variants reveal patterns of chromosome evolution in
ancient Cycadales genomes

J 2022

Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes

VOZÁROVÁ, Radka; Wencai WANG; Jana LUNEROVÁ; Fengqing SHAO; Jaume PELLICER et al.

Základní údaje

Originální název

Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes

Autoři

VOZÁROVÁ, Radka; Wencai WANG; Jana LUNEROVÁ; Fengqing SHAO; Jaume PELLICER; Ilia J. LEITCH; Andrew R. LEITCH a Aleš KOVAŘÍK

Vydání

The Plant Journal, Wiley, 2022, 0960-7412

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00126840

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Telomeres; genome evolution; chromosome rearrangements; Cycadaceae; gymnosperms; centromeres; epigenetics

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2025 14:28, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Simple telomeric repeats composed of 6-7 iterating nucleotide units are important sequences typically found at the ends of chromosomes. Here we analyzed their abundance and homogeneity in 42 gymnosperm (29 newly sequenced), 29 angiosperm (1 newly sequenced) and 8 bryophytes using bioinformatics, conventional cytogenetic and molecular biology approaches to explore their diversity across land plants. We found more than 10,000-fold variation in the amounts of telomeric repeats among the investigated taxa. Repeat abundance was positively correlated with increasing intragenomic sequence heterogeneity and occurrence at non-telomeric positions, but there was no correlation with genome size. The highest abundance/heterogeneity was found in the gymnosperm genus Cycas (Cycadaceae), in which megabase-sized blocks of telomeric repeats (i.e. billions of copies) were identified. Fluorescent in situ hybridization (FISH) experiments using variant-specific probes revealed canonical Arabidopsis-type telomeric TTTAGGG repeats at chromosome ends, while pericentromeric blocks comprised at least four major telomeric variants with decreasing abundance: TTTAGGG>TTCAGGG >TTTAAGG>TTCAAGG. Such a diversity of repeats was not found in the sister cycad family Zamiaceae or in any other species analyzed. Using immunocytochemistry, we showed that the pericentromeric blocks of telomeric repeats overlapped with histone H3 serine 10 phosphorylation signals. We show that species of Cycas have amplified their telomeric repeats in centromeric and telomeric positions on telocentric chromosomes to extraordinary high levels. Ancestral chromosome number reconstruction suggests their occurrence is unlikely to be the product of ancient Robertsonian chromosome fusions. We speculate as to how the observed chromosome dynamics may be associated with the diversification of cycads.

Návaznosti

90131, velká výzkumná infrastruktura

Název: ELIXIR-CZ II

Citovat

VOZÁROVÁ, Radka; Wencai WANG; Jana LUNEROVÁ; Fengqing SHAO; Jaume PELLICER; Ilia J. LEITCH; Andrew R. LEITCH a Aleš KOVAŘÍK. Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes. The Plant Journal. Wiley, 2022, roč. 112, č. 3, s. 646-663. ISSN 0960-7412. Dostupné z: https://doi.org/10.1111/tpj.15969.

@article{2223805,
   author = {Vozárová, Radka and Wang, Wencai and Lunerová, Jana and Shao, Fengqing and Pellicer, Jaume and Leitch, Ilia J. and Leitch, Andrew R. and Kovařík, Aleš},
   article_number = {3},
   doi = {https://doi.org/10.1111/tpj.15969},
   keywords = {Telomeres; genome evolution; chromosome rearrangements; Cycadaceae; gymnosperms; centromeres; epigenetics},
   language = {eng},
   issn = {0960-7412},
   journal = {The Plant Journal},
   title = {Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.15969},
   volume = {112},
   year = {2022}
}

TY  - JOUR
ID  - 2223805
AU  - Vozárová, Radka - Wang, Wencai - Lunerová, Jana - Shao, Fengqing - Pellicer, Jaume - Leitch, Ilia J. - Leitch, Andrew R. - Kovařík, Aleš
PY  - 2022
TI  - Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes
JF  - The Plant Journal
VL  - 112
IS  - 3
SP  - 646-663
EP  - 646-663
PB  - Wiley
SN  - 09607412
KW  - Telomeres
KW  - genome evolution
KW  - chromosome rearrangements
KW  - Cycadaceae
KW  - gymnosperms
KW  - centromeres
KW  - epigenetics
UR  - https://onlinelibrary.wiley.com/doi/10.1111/tpj.15969
N2  - Simple telomeric repeats composed of 6-7 iterating nucleotide units are important sequences typically found at the ends of chromosomes. Here we analyzed their abundance and homogeneity in 42 gymnosperm (29 newly sequenced), 29 angiosperm (1 newly sequenced) and 8 bryophytes using bioinformatics, conventional cytogenetic and molecular biology approaches to explore their diversity across land plants. We found more than 10,000-fold variation in the amounts of telomeric repeats among the investigated taxa. Repeat abundance was positively correlated with increasing intragenomic sequence heterogeneity and occurrence at non-telomeric positions, but there was no correlation with genome size. The highest abundance/heterogeneity was found in the gymnosperm genus Cycas (Cycadaceae), in which megabase-sized blocks of telomeric repeats (i.e. billions of copies) were identified. Fluorescent in situ hybridization (FISH) experiments using variant-specific probes revealed canonical Arabidopsis-type telomeric TTTAGGG repeats at chromosome ends, while pericentromeric blocks comprised at least four major telomeric variants with decreasing abundance: TTTAGGG>TTCAGGG >TTTAAGG>TTCAAGG. Such a diversity of repeats was not found in the sister cycad family Zamiaceae or in any other species analyzed. Using immunocytochemistry, we showed that the pericentromeric blocks of telomeric repeats overlapped with histone H3 serine 10 phosphorylation signals. We show that species of Cycas have amplified their telomeric repeats in centromeric and telomeric positions on telocentric chromosomes to extraordinary high levels. Ancestral chromosome number reconstruction suggests their occurrence is unlikely to be the product of ancient Robertsonian chromosome fusions. We speculate as to how the observed chromosome dynamics may be associated with the diversification of cycads.
ER  -

VOZÁROVÁ, Radka; Wencai WANG; Jana LUNEROVÁ; Fengqing SHAO; Jaume PELLICER; Ilia J. LEITCH; Andrew R. LEITCH a Aleš KOVAŘÍK. Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes. \textit{The Plant Journal}. Wiley, 2022, roč.~112, č.~3, s.~646-663. ISSN~0960-7412. Dostupné z: https://doi.org/10.1111/tpj.15969.

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