Actin cytoskeleton in two human fungal pathogens Aureobasidium
pullulans and Cryptococcus neoformans.

D 1999

Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans.

KOPECKÁ, Marie; Kanji TAKEO; Miroslav GABRIEL a Augustin SVOBODA

Základní údaje

Originální název

Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans.

Autoři

KOPECKÁ, Marie; Kanji TAKEO; Miroslav GABRIEL a Augustin SVOBODA

Vydání

1999. vyd. Bratislava, 20th International Specialized Symposium on Yeasts, s. 38-38, 1999

Nakladatel

Czech and Slovak Yeast Commission of the Czechoslovak Society for Microbiology

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/99:00003238

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

actin cytoskeleton; human pathogenic fungi; Cryptococcus neoformans; Aureobasidium pullulans

Štítky

actin cytoskeleton, Aureobasidium pullulans, Cryptococcus neoformans, human pathogenic fungi

Změněno: 28. 2. 2001 12:52, doc. MUDr. Miroslav Gabriel, CSc.

Anotace

V originále

Two strains of human fungal pathogen Cryptococus neoformans and three strains of oportunistic pathogen Aureobasidium pullulans were examined by rhodamine-phalloidin and DAPI staining after paraformaldehyde fixation and Triton X-100 treatment to identify actin cytoskeleton structures and nuclei.Uninuclear C. neoformans cells revealed actin cables oriented to developing bud, actin patches accumulated at growing bud and actin ring in the neck region between mother and daughter cells. In contrast, opportunistic pathogen A.pullulans did not reveal actin cables or patches, while actin rings were seen in multinuclear cells.However, all cells revealed diffuse F-actin cytoplasmic staining, accumulated at tips of young hyphae that may represent simple actin cytoskeleton enabling variable morphogenetic changes. Occurence of multinuclear cells in absence of actin cables supports our hypothesis on the role of actin cables in positioning of cell nucleus.

Návaznosti

GA204/97/1150, projekt VaV

Název: Buněčná stěna a morfogeneze kvasinkové buňky: role cytoskeletu

Investor: Grantová agentura ČR, Buněčná stěna a morfogeneze kvasinkové buňky: role cytoskeletu

Citovat

KOPECKÁ, Marie; Kanji TAKEO; Miroslav GABRIEL a Augustin SVOBODA. Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans. In 20th International Specialized Symposium on Yeasts. 1999. vyd. Bratislava: Czech and Slovak Yeast Commission of the Czechoslovak Society for Microbiology, 1999, s. 38.

@inproceedings{222411,
   author = {Kopecká, Marie and Takeo, Kanji and Gabriel, Miroslav and Svoboda, Augustin},
   address = {Bratislava},
   booktitle = {20th International Specialized Symposium on Yeasts},
   edition = {1999},
   keywords = {actin cytoskeleton; human pathogenic fungi; Cryptococcus neoformans; Aureobasidium pullulans},
   language = {eng},
   location = {Bratislava},
   pages = {38-38},
   publisher = {Czech and Slovak Yeast Commission of the Czechoslovak Society for Microbiology},
   title = {Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans.},
   year = {1999}
}

TY  - CONF
ID  - 222411
AU  - Kopecká, Marie - Takeo, Kanji - Gabriel, Miroslav - Svoboda, Augustin
PY  - 1999
TI  - Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans.
PB  - Czech and Slovak Yeast Commission of the Czechoslovak Society for Microbiology
CY  - Bratislava
KW  - actin cytoskeleton
KW  - human pathogenic fungi
KW  - Cryptococcus neoformans
KW  - Aureobasidium pullulans
N2  - Two strains of human fungal pathogen Cryptococus neoformans and three strains of oportunistic pathogen Aureobasidium pullulans were examined by rhodamine-phalloidin and DAPI staining after paraformaldehyde fixation and Triton X-100 treatment to identify actin cytoskeleton structures and nuclei.Uninuclear C. neoformans cells revealed actin cables oriented to developing bud, actin patches accumulated at growing bud and actin ring in the neck region between mother and daughter cells. In contrast, opportunistic pathogen A.pullulans did not reveal actin cables or patches, while actin rings were seen in multinuclear cells.However, all cells revealed diffuse F-actin cytoplasmic staining, accumulated at tips of young hyphae that may represent simple actin cytoskeleton enabling variable morphogenetic changes. Occurence of multinuclear cells in absence of actin cables supports our hypothesis on the role of actin cables in positioning of cell nucleus.
ER  -

KOPECKÁ, Marie; Kanji TAKEO; Miroslav GABRIEL a Augustin SVOBODA. Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans. In \textit{20th International Specialized Symposium on Yeasts}. 1999. vyd. Bratislava: Czech and Slovak Yeast Commission of the Czechoslovak Society for Microbiology, 1999, s.~38.

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