Bacteria? You shall not PASs!

a 2022

Bacteria? You shall not PASs!

KUČEROVÁ, Eliška, Tibor BOTKA a Jiřina HAVRÁNKOVÁ

Základní údaje

Originální název

Bacteria? You shall not PASs!

Autoři

KUČEROVÁ, Eliška (203 Česká republika, domácí), Tibor BOTKA (203 Česká republika, garant, domácí) a Jiřina HAVRÁNKOVÁ (203 Česká republika, domácí)

Vydání

The Biomania 2022 Student Scientific Meeting, Brno, Czech Republic, 2022, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Biomania 2022

Kód RIV

RIV/00216224:14310/22:00129693

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0040-0

Klíčová slova anglicky

phage-antibiotic synergy; PAS; Staphylococcus aureus

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 11. 2022 15:06, Mgr. Tibor Botka, Ph.D.

Anotace

V originále

The increasing prevalence of multidrug-resistant bacteria and the rise of the postantibiotic era show phage therapy as a promising alternative for antibiotic treatment of bacterial infections. The main limitations of the development and production of phage preparations are regulatory requirements, including the quality and effectiveness proof. One of the new strategies to overcome bacterial resistance is the synergy between antibiotics and bacteriophage therapeutics (phage-antibiotic synergy; PAS). The presented work is concerned about new methods of the PAS evaluation. The development of these methods is crucial nowadays, not just because of fulfilling legislative requirements, but also because of the possibility of automatizing the whole process. In this work, the efficacy of two distinct therapeutic phages, 812 and P68, in combination with the three most promising antibiotics in eradication of four Staphylococcus aureus strains, including laboratory and clinical isolates, was evaluated. Experiments were performed under different conditions, e.g. phage:strain input ratios and antibiotic concentrations. All the used antibiotics affect bacterial cell wall metabolism, but their mechanism of action differs. Based on the results obtained by stationary experiments on microbiology dishes, a spectrophotometric method using a TECAN Infinite 200 plate reader followed. The data obtained will be compared with those measured by other methods not previously used for this purpose.

Návaznosti

NU21J-05-00035, projekt VaV

Název: Synergie lytických bakteriofágů a antibiotik v léčbě povrchových infekcí způsobených Staphylococcus aureus (Akronym: PAS-based therapy of S. aureus infections)

Investor: Ministerstvo zdravotnictví ČR, Synergy of lytic bacteriophages and antibiotics in the therapy of topical infections of Staphylococcus aureus, Podprogram 2 - juniorský - výzkumníci do 35 let

Citovat

KUČEROVÁ, Eliška, Tibor BOTKA a Jiřina HAVRÁNKOVÁ. Bacteria? You shall not PASs! In The Biomania 2022 Student Scientific Meeting, Brno, Czech Republic, 2022. 2022. ISBN 978-80-280-0040-0.

@proceedings{2225542,
   author = {Kučerová, Eliška and Botka, Tibor and Havránková, Jiřina},
   booktitle = {The Biomania 2022 Student Scientific Meeting, Brno, Czech Republic, 2022},
   keywords = {phage-antibiotic synergy; PAS; Staphylococcus aureus},
   language = {eng},
   isbn = {978-80-280-0040-0},
   title = {Bacteria? You shall not PASs!},
   url = {http://www.biomania.cz/},
   year = {2022}
}

TY  - CONF
ID  - 2225542
AU  - Kučerová, Eliška - Botka, Tibor - Havránková, Jiřina
PY  - 2022
TI  - Bacteria? You shall not PASs!
SN  - 9788028000400
KW  - phage-antibiotic synergy
KW  - PAS
KW  - Staphylococcus aureus
UR  - http://www.biomania.cz/
N2  - The increasing prevalence of multidrug-resistant bacteria and the rise of the postantibiotic era show phage therapy as a promising alternative for antibiotic treatment of bacterial infections. The main limitations of the development and production of phage preparations are regulatory requirements, including the quality and effectiveness proof. One of the new strategies to overcome bacterial resistance is the synergy between antibiotics and bacteriophage therapeutics (phage-antibiotic synergy; PAS). The presented work is concerned about new methods of the PAS evaluation. The development of these methods is crucial nowadays, not just because of fulfilling legislative requirements, but also because of the possibility of automatizing the whole process. In this work, the efficacy of two distinct therapeutic phages, 812 and P68, in combination with the three most promising antibiotics in eradication of four Staphylococcus aureus strains, including laboratory and clinical isolates, was evaluated. Experiments were performed under different conditions, e.g. phage:strain input ratios and antibiotic concentrations. All the used antibiotics affect bacterial cell wall metabolism, but their mechanism of action differs. Based on the results obtained by stationary experiments on microbiology dishes, a spectrophotometric method using a TECAN Infinite 200 plate reader followed. The data obtained will be compared with those measured by other methods not previously used for this purpose.
ER  -

KUČEROVÁ, Eliška, Tibor BOTKA a Jiřina HAVRÁNKOVÁ. Bacteria? You shall not PASs! In \textit{The Biomania 2022 Student Scientific Meeting, Brno, Czech Republic, 2022}. 2022. ISBN~978-80-280-0040-0.

Podrobný výpis o publikaci