Cryo-electron microscopy and image classification reveal the
existence and structure of the coxsackievirus A6 virion

J 2022

Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion

BÜTTNER, Carina Renate; Radovan SPURNÝ; Tibor FÜZIK a Pavel PLEVKA

Základní údaje

Originální název

Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion

Autoři

BÜTTNER, Carina Renate; Radovan SPURNÝ; Tibor FÜZIK a Pavel PLEVKA

Vydání

Communications Biology, Nature Research, 2022, 2399-3642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/22:00127051

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

cryoelectron microscopy; Enterovirus; genetics; hand foot and mouth disease; human; virion

Štítky

CF CELLIM, CF CRYO, NIVB_CEITEC, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 10. 2024 09:56, Ing. Jana Kuchtová

Anotace

V originále

Coxsackievirus A6 (CV-A6) has recently overtaken enterovirus A71 and CV-A16 as the primary causative agent of hand, foot, and mouth disease worldwide. Virions of CV-A6 were not identified in previous structural studies, and it was speculated that the virus is unique among enteroviruses in using altered particles with expanded capsids to infect cells. In contrast, the virions of other enteroviruses are required for infection. Here we used cryo-electron microscopy (cryo-EM) to determine the structures of the CV-A6 virion, altered particle, and empty capsid. We show that the CV-A6 virion has features characteristic of virions of other enteroviruses, including a compact capsid, VP4 attached to the inner capsid surface, and fatty acid-like molecules occupying the hydrophobic pockets in VP1 subunits. Furthermore, we found that in a purified sample of CV-A6, the ratio of infectious units to virions is 1 to 500. Therefore, it is likely that virions of CV-A6 initiate infection, like those of other enteroviruses. Our results provide evidence that future vaccines against CV-A6 should target its virions instead of the antigenically distinct altered particles. Furthermore, the structure of the virion provides the basis for the rational development of capsid-binding inhibitors that block the genome release of CV-A6. A cryo-EM structure for the three conformers of coxsackievirus A6 provides insight into the infection process of this enterovirus, which is responsible for numerous cases of hand, foot, and mouth disease.

Návaznosti

GX19-25982X, projekt VaV

Název: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Grantová agentura ČR, Structural study of enterovirus replication in situ

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

90127, velká výzkumná infrastruktura

Název: CIISB II

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Přiložené soubory

s42003-022-03863-2.pdf Verze souboru

Citovat

BÜTTNER, Carina Renate; Radovan SPURNÝ; Tibor FÜZIK a Pavel PLEVKA. Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion. Communications Biology. Nature Research, 2022, roč. 5, č. 1, s. 898-907. ISSN 2399-3642. Dostupné z: https://doi.org/10.1038/s42003-022-03863-2.

@article{2228318,
   author = {Büttner, Carina Renate and Spurný, Radovan and Füzik, Tibor and Plevka, Pavel},
   article_number = {1},
   doi = {https://doi.org/10.1038/s42003-022-03863-2},
   keywords = {cryoelectron microscopy; Enterovirus; genetics; hand foot and mouth disease; human; virion},
   language = {eng},
   issn = {2399-3642},
   journal = {Communications Biology},
   title = {Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion},
   url = {https://www.nature.com/articles/s42003-022-03863-2},
   volume = {5},
   year = {2022}
}

TY  - JOUR
ID  - 2228318
AU  - Büttner, Carina Renate - Spurný, Radovan - Füzik, Tibor - Plevka, Pavel
PY  - 2022
TI  - Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion
JF  - Communications Biology
VL  - 5
IS  - 1
SP  - 898
EP  - 898
PB  - Nature Research
SN  - 23993642
KW  - cryoelectron microscopy
KW  - Enterovirus
KW  - genetics
KW  - hand foot and mouth disease
KW  - human
KW  - virion
UR  - https://www.nature.com/articles/s42003-022-03863-2
N2  - Coxsackievirus A6 (CV-A6) has recently overtaken enterovirus A71 and CV-A16 as the primary causative agent of hand, foot, and mouth disease worldwide. Virions of CV-A6 were not identified in previous structural studies, and it was speculated that the virus is unique among enteroviruses in using altered particles with expanded capsids to infect cells. In contrast, the virions of other enteroviruses are required for infection. Here we used cryo-electron microscopy (cryo-EM) to determine the structures of the CV-A6 virion, altered particle, and empty capsid. We show that the CV-A6 virion has features characteristic of virions of other enteroviruses, including a compact capsid, VP4 attached to the inner capsid surface, and fatty acid-like molecules occupying the hydrophobic pockets in VP1 subunits. Furthermore, we found that in a purified sample of CV-A6, the ratio of infectious units to virions is 1 to 500. Therefore, it is likely that virions of CV-A6 initiate infection, like those of other enteroviruses. Our results provide evidence that future vaccines against CV-A6 should target its virions instead of the antigenically distinct altered particles. Furthermore, the structure of the virion provides the basis for the rational development of capsid-binding inhibitors that block the genome release of CV-A6. A cryo-EM structure for the three conformers of coxsackievirus A6 provides insight into the infection process of this enterovirus, which is responsible for numerous cases of hand, foot, and mouth disease.
ER  -

BÜTTNER, Carina Renate; Radovan SPURNÝ; Tibor FÜZIK a Pavel PLEVKA. Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion. \textit{Communications Biology}. Nature Research, 2022, roč.~5, č.~1, s.~898-907. ISSN~2399-3642. Dostupné z: https://doi.org/10.1038/s42003-022-03863-2.

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