Preparative protein concentration from biofluids by
epitachophoresis

J 2022

Preparative protein concentration from biofluids by epitachophoresis

HRUŠKOVÁ, Helena; Ivona VORÁČOVÁ; Markéta LAŠTOVIČKOVÁ; Michael KILLINGER; František FORET et al.

Základní údaje

Originální název

Preparative protein concentration from biofluids by epitachophoresis

Autoři

HRUŠKOVÁ, Helena; Ivona VORÁČOVÁ; Markéta LAŠTOVIČKOVÁ; Michael KILLINGER a František FORET

Vydání

Journal of Chromatography A, Elsevier B.V. 2022, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00127094

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Proteins; Epitachophoresis; Concentration; Urine; Growth medium

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 1. 2023 10:14, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

With increasing demands on protein analyses in complex biological matrices, the insistence on developing new sample preparation techniques is rising. Recently, we introduced a new displacement electrophoresis technique (epitachophoresis) and instrumentation for preparative concentration and cleaning of DNA samples. This work describes the possibility of applying this device to protein samples. We have developed a method for the epitachophoretic concentration of proteins in a cationic mode and tested it by concentrating and collecting the protein zones from complex biological matrices (urine and growth medium). Under optimized conditions, we have obtained recoveries up to 99%. Furthermore, the applicability of the developed method was proven by concentrating and collecting the cytochrome c zone from a HeLa cell line growth medium, where the protein cytochrome c was released during cell apoptosis.

Citovat

HRUŠKOVÁ, Helena; Ivona VORÁČOVÁ; Markéta LAŠTOVIČKOVÁ; Michael KILLINGER a František FORET. Preparative protein concentration from biofluids by epitachophoresis. Journal of Chromatography A. Elsevier B.V., 2022, roč. 1685, December, s. 1-6. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2022.463591.

@article{2229941,
   author = {Hrušková, Helena and Voráčová, Ivona and Laštovičková, Markéta and Killinger, Michael and Foret, František},
   article_number = {December},
   doi = {https://doi.org/10.1016/j.chroma.2022.463591},
   keywords = {Proteins; Epitachophoresis; Concentration; Urine; Growth medium},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Preparative protein concentration from biofluids by epitachophoresis},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967322007828?via%3Dihub},
   volume = {1685},
   year = {2022}
}

TY  - JOUR
ID  - 2229941
AU  - Hrušková, Helena - Voráčová, Ivona - Laštovičková, Markéta - Killinger, Michael - Foret, František
PY  - 2022
TI  - Preparative protein concentration from biofluids by epitachophoresis
JF  - Journal of Chromatography A
VL  - 1685
IS  - December
SP  - 1-6
EP  - 1-6
PB  - Elsevier B.V.
SN  - 00219673
KW  - Proteins
KW  - Epitachophoresis
KW  - Concentration
KW  - Urine
KW  - Growth medium
UR  - https://www.sciencedirect.com/science/article/pii/S0021967322007828?via%3Dihub
N2  - With increasing demands on protein analyses in complex biological matrices, the insistence on developing new sample preparation techniques is rising. Recently, we introduced a new displacement electrophoresis technique (epitachophoresis) and instrumentation for preparative concentration and cleaning of DNA samples. This work describes the possibility of applying this device to protein samples. We have developed a method for the epitachophoretic concentration of proteins in a cationic mode and tested it by concentrating and collecting the protein zones from complex biological matrices (urine and growth medium). Under optimized conditions, we have obtained recoveries up to 99%. Furthermore, the applicability of the developed method was proven by concentrating and collecting the cytochrome c zone from a HeLa cell line growth medium, where the protein cytochrome c was released during cell apoptosis.
ER  -

HRUŠKOVÁ, Helena; Ivona VORÁČOVÁ; Markéta LAŠTOVIČKOVÁ; Michael KILLINGER a František FORET. Preparative protein concentration from biofluids by epitachophoresis. \textit{Journal of Chromatography A}. Elsevier B.V., 2022, roč.~1685, December, s.~1-6. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2022.463591.

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