Detection of cancer biomarkers using immunoassays with
upconversion nanoparticles and magnetic preconcentration

a 2022

Detection of cancer biomarkers using immunoassays with upconversion nanoparticles and magnetic preconcentration

SKLENÁROVÁ, Dorota; Ekaterina MAKHNEVA; Julian BRANDMEIER; Hans-Heiner GORRIS; Zdeněk FARKA et al.

Základní údaje

Originální název

Detection of cancer biomarkers using immunoassays with upconversion nanoparticles and magnetic preconcentration

Název česky

Detekce rakovinných biomarkerů pomocí imunostanovení s upkonverzními nanočásticemi a magnetickou prekoncentrací

Autoři

SKLENÁROVÁ, Dorota; Ekaterina MAKHNEVA; Julian BRANDMEIER; Hans-Heiner GORRIS a Zdeněk FARKA

Vydání

21. Interdisciplinary meeting of young life scientists, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISSN

Klíčová slova anglicky

immunoassay; magnetic microparticles; cancer biomarker; prostate-specific antigen; photon-upconversion nanoparticles

Změněno: 10. 11. 2022 15:23, Mgr. Dorota Sklenárová

Anotace

V originále

Prostate cancer is the major cause of death in the male population caused by oncologic diseases. Consequently, sensitive detection of disease biomarkers, e.g., prostate-specific antigen (PSA), is essential for early-stage diagnosis and effective treatment. In clinical analysis, immunochemical methods such as enzyme-linked immunosorbent assay (ELISA) are widely used. However, conventional ELISA is often not sensitive enough for early-stage diagnosis, resulting in the need for alternative approaches. Photon-upconversion nanoparticles (UCNPs) are unique for their anti-Stokes luminescence, because NIR excitation results in the emission of UV/vis light without optical background interference due to autofluorescence. Furthermore, the surface of UCNPs can be modified with a variety of biomolecules such as streptavidin or antibodies. These labels can be used in various immunoassay formats such as the upconversion-linked immunosorbent assay (ULISA). However, to improve the sensitivity even further, it is necessary to search for new methods. Magnetic microparticles (beads) are a promising alternative for microtiter plate-based immunoassays. Their superparamagnetic properties allow for analyte preconcentration, thus improving the assay sensitivity. We have developed an upconversion-based sandwich immunoassay using magnetic preconcentration for the detection of PSA. Our recent results have reached a limit of detection in the order of 1 pg/mL, which is approximately 20-fold better compared to an ELISA using the same immunoreagents. The higher sensitivity demonstrates the potential of using magnetic microparticles combined with UCNPs for the immunochemical detection of cancer biomarkers.

Návaznosti

GA21-03156S, projekt VaV

Název: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Grantová agentura ČR, Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Citovat

SKLENÁROVÁ, Dorota; Ekaterina MAKHNEVA; Julian BRANDMEIER; Hans-Heiner GORRIS a Zdeněk FARKA. Detection of cancer biomarkers using immunoassays with upconversion nanoparticles and magnetic preconcentration. In 21. Interdisciplinary meeting of young life scientists. 2022. ISSN 2336-7202.

@proceedings{2230757,
   author = {Sklenárová, Dorota and Makhneva, Ekaterina and Brandmeier, Julian and Gorris, HansandHeiner and Farka, Zdeněk},
   booktitle = {21. Interdisciplinary meeting of young life scientists},
   keywords = {immunoassay; magnetic microparticles; cancer biomarker; prostate-specific antigen; photon-upconversion nanoparticles},
   language = {eng},
   title = {Detection of cancer biomarkers using immunoassays with upconversion nanoparticles and magnetic preconcentration},
   url = {http://www.ccsss.cz/index.php/ccsss/issue/view/31/45},
   year = {2022}
}

TY  - CONF
ID  - 2230757
AU  - Sklenárová, Dorota - Makhneva, Ekaterina - Brandmeier, Julian - Gorris, Hans-Heiner - Farka, Zdeněk
PY  - 2022
TI  - Detection of cancer biomarkers using immunoassays with upconversion nanoparticles and magnetic preconcentration
KW  - immunoassay
KW  - magnetic microparticles
KW  - cancer biomarker
KW  - prostate-specific antigen
KW  - photon-upconversion nanoparticles
UR  - http://www.ccsss.cz/index.php/ccsss/issue/view/31/45
N2  - Prostate cancer is the major cause of death in the male population caused by oncologic diseases. Consequently, sensitive detection of disease biomarkers, e.g., prostate-specific antigen (PSA), is essential for early-stage diagnosis and effective treatment. In clinical analysis, immunochemical methods such as enzyme-linked immunosorbent assay (ELISA) are widely used. However, conventional ELISA is often not sensitive enough for early-stage diagnosis, resulting in the need for alternative approaches. Photon-upconversion nanoparticles (UCNPs) are unique for their anti-Stokes luminescence, because NIR excitation results in the emission of UV/vis light without optical background interference due to autofluorescence. Furthermore, the surface of UCNPs can be modified with a variety of biomolecules such as streptavidin or antibodies. These labels can be used in various immunoassay formats such as the upconversion-linked immunosorbent assay (ULISA). However, to improve the sensitivity even further, it is necessary to search for new methods. Magnetic microparticles (beads) are a promising alternative for microtiter plate-based immunoassays. Their superparamagnetic properties allow for analyte preconcentration, thus improving the assay sensitivity. We have developed an upconversion-based sandwich immunoassay using magnetic preconcentration for the detection of PSA. Our recent results have reached a limit of detection in the order of 1 pg/mL, which is approximately 20-fold better compared to an ELISA using the same immunoreagents. The higher sensitivity demonstrates the potential of using magnetic microparticles combined with UCNPs for the immunochemical detection of cancer biomarkers.
ER  -

SKLENÁROVÁ, Dorota; Ekaterina MAKHNEVA; Julian BRANDMEIER; Hans-Heiner GORRIS a Zdeněk FARKA. Detection of cancer biomarkers using immunoassays with upconversion nanoparticles and magnetic preconcentration. In \textit{21. Interdisciplinary meeting of young life scientists}. 2022. ISSN~2336-7202.

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