The position of centromeres in interphase nuclei of human
leukemic cells during myeloid differentiation and after
gamma-irradiation

D 1999

The position of centromeres in interphase nuclei of human leukemic cells during myeloid differentiation and after gamma-irradiation

BÁRTOVÁ, Eva; Stanislav KOZUBEK; Markus DURM; Michael HAUSMANN; Michal KOZUBEK et al.

Základní údaje

Originální název

The position of centromeres in interphase nuclei of human leukemic cells during myeloid differentiation and after gamma-irradiation

Autoři

Vydání

vol.3. Ústí nad Labem, Fluorescence microscopy and fluorescent probes, s. 347-354, 1999

Nakladatel

Espero Publishing

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10610 Biophysics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14330/99:00001458

Organizační jednotka

Fakulta informatiky

ISBN

80-238-4668-X

Klíčová slova anglicky

centromeres

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 5. 2010 17:14, prof. RNDr. Michal Kozubek, Ph.D.

Anotace

V originále

Centromeres are frequently associated with heterochromatin and other chromatin components that cause the epigenetic transcriptional repression of genes. Heterochromatin is often found in close association with the nuclear periphery and tends to replicate late in the S phase of the cell cycle. Centromere-induced gene silencing also interferes with chromatin segregation, proving a critical link between chromatin structure and centromere function. The aim of this study is to analyse the spatial organisation of centromeres by means of a newly developed automated fluorescence microscopy system (Brno high resolution cytometer) in combination with Fast-Fluorescence in situ Hybridisation (FAST-FISH). The latter has been recently developed as an easy to handle technique especially for centromere labelling. In the experiments the positions of some human centromeres during myeloid differentiation and after gamma-irradiation were detected and analysed. Methodologically, the results indicate that due to high reproducibility and signal intensity, FAST-FISH was useful for automated microscopy and image analysis by the Brno high resolution cytometer. The human leukemic cell line U937 blocked on the level of promyelocytes was differentiated in granulocytes with all-trans retinoic acid (ATRA) and dimethyl sulfoxide (DMSO) and then into monocytes-macrophages with phorbol esters (PMA). The U937 cells were also irradiated with 5 Gy gamma rays (60Co) to determine the topology of some centromeres after radiation treatment. The positions of the centromeres of the human chromosomes 8, 9, 12, 17, 18 and X were determined and compared to the untreated control. The proximity of homologous and heterologous centromeres was detected. Generally, during granulocytic differentiation the positions of centromeres did not change significantly, but in monocytic differentiation some centromeres were closer to each other and closer to the nuclear centre. Changes in the nuclear topology of human centromeres were found after gamma irradiation, the centromeres were repositioned closer to the nuclear centre (repositioning about 9-19% of nuclear radius).

Návaznosti

MSM 143300002, záměr

Název: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Využití počítačové analýzy obrazu v optické mikroskopii

VS97031, projekt VaV

Název: Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka

Citovat

BÁRTOVÁ, Eva; Stanislav KOZUBEK; Markus DURM; Michael HAUSMANN; Michal KOZUBEK; Emilie LUKÁŠOVÁ; Magdalena SKALNÍKOVÁ; Pavla JIRSOVÁ; Alena CAFOURKOVÁ a Kateřina BUCHNÍČKOVÁ. The position of centromeres in interphase nuclei of human leukemic cells during myeloid differentiation and after gamma-irradiation. edited by A.Kotyk. In Fluorescence microscopy and fluorescent probes. vol.3. Ústí nad Labem: Espero Publishing, 1999, s. 347-354. ISBN 80-238-4668-X.

@inproceedings{223551,
   author = {Bártová, Eva and Kozubek, Stanislav and Durm, Markus and Hausmann, Michael and Kozubek, Michal and Lukášová, Emilie and Skalníková, Magdalena and Jirsová, Pavla and Cafourková, Alena and Buchníčková, Kateřina},
   address = {Ústí nad Labem},
   booktitle = {Fluorescence microscopy and fluorescent probes},
   edition = {vol.3},
   keywords = {centromeres},
   language = {eng},
   location = {Ústí nad Labem},
   isbn = {80-238-4668-X},
   pages = {347-354},
   publisher = {Espero Publishing},
   title = {The position of centromeres in interphase nuclei of human leukemic cells during myeloid differentiation and after gamma-irradiation},
   year = {1999}
}

TY  - CONF
ID  - 223551
AU  - Bártová, Eva - Kozubek, Stanislav - Durm, Markus - Hausmann, Michael - Kozubek, Michal - Lukášová, Emilie - Skalníková, Magdalena - Jirsová, Pavla - Cafourková, Alena - Buchníčková, Kateřina
PY  - 1999
TI  - The position of centromeres in interphase nuclei of human leukemic cells during myeloid differentiation and after gamma-irradiation
PB  - Espero Publishing
CY  - Ústí nad Labem
SN  - 802384668X
KW  - centromeres
N2  - Centromeres are frequently associated with heterochromatin and other chromatin components that cause the epigenetic transcriptional repression of genes. Heterochromatin is often found in close association with the nuclear periphery and tends to replicate late in the S phase of the cell cycle. Centromere-induced gene silencing also interferes with chromatin segregation, proving a critical link between chromatin structure and centromere function. The aim of this study is to analyse the spatial organisation of centromeres by means of a newly developed automated fluorescence microscopy system (Brno high resolution cytometer) in combination with Fast-Fluorescence in situ Hybridisation (FAST-FISH). The latter has been recently developed as an easy to handle technique especially for centromere labelling. In the experiments the positions of some human centromeres during myeloid differentiation and after gamma-irradiation were detected and analysed. Methodologically, the results indicate that due to high reproducibility and signal intensity, FAST-FISH was useful for automated microscopy and image analysis by the Brno high resolution cytometer. The human leukemic cell line U937 blocked on the level of promyelocytes was differentiated in granulocytes with all-trans retinoic acid (ATRA) and dimethyl sulfoxide (DMSO) and then into monocytes-macrophages with phorbol esters (PMA). The U937 cells were also irradiated with 5 Gy gamma rays (60Co) to determine the topology of some centromeres after radiation treatment. The positions of the centromeres of the human chromosomes 8, 9, 12, 17, 18 and X were determined and compared to the untreated control. The proximity of homologous and heterologous centromeres was detected. Generally, during granulocytic differentiation the positions of centromeres did not change significantly, but in monocytic differentiation some centromeres were closer to each other and closer to the nuclear centre. Changes in the nuclear topology of human centromeres were found after gamma irradiation, the centromeres were repositioned closer to the nuclear centre (repositioning about 9-19% of nuclear radius).
ER  -

BÁRTOVÁ, Eva; Stanislav KOZUBEK; Markus DURM; Michael HAUSMANN; Michal KOZUBEK; Emilie LUKÁŠOVÁ; Magdalena SKALNÍKOVÁ; Pavla JIRSOVÁ; Alena CAFOURKOVÁ a Kateřina BUCHNÍČKOVÁ. The position of centromeres in interphase nuclei of human leukemic cells during myeloid differentiation and after gamma-irradiation. edited by A.Kotyk. In \textit{Fluorescence microscopy and fluorescent probes}. vol.3. Ústí nad Labem: Espero Publishing, 1999, s.~347-354. ISBN~80-238-4668-X.

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