2022
Selenium nanoparticles with boron salt-based compound act synergistically against the brown-rot Serpula lacrymans
GABLECH, Evelína; Zdenka FOHLEROVÁ; Karel ŠVEC; František ZALEŠ; Oldřich BENADA et. al.Základní údaje
Originální název
Selenium nanoparticles with boron salt-based compound act synergistically against the brown-rot Serpula lacrymans
Autoři
GABLECH, Evelína; Zdenka FOHLEROVÁ; Karel ŠVEC; František ZALEŠ; Oldřich BENADA; Olga KOFROŇOVÁ; Jana PEKÁRKOVÁ; Ondřej CAHA (203 Česká republika, domácí); Imrich GABLECH; Jiří GABRIEL a Jana DRBOHLAVOVÁ (garant)
Vydání
International Biodeterioration and Biodegradation, Elsevier Ltd, 2022, 0964-8305
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10302 Condensed matter physics
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 4.800
Kód RIV
RIV/00216224:14310/22:00127344
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000821274600003
EID Scopus
2-s2.0-85123781773
Klíčová slova anglicky
Selenium nanoparticle; Serpula lacrymans; Fungicide; brown-rot fungi; Cytotoxicity; Boron salt
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 5. 12. 2022 11:47, Mgr. Marie Novosadová Šípková, DiS.
Anotace
V originále
The spherical polyvinylpyrrolidone (PVP) stabilized red Se NPs (PVP–Se NPs) with a mean size of ∼45 nm were prepared via upgraded one-step wet chemical reduction by L-cysteine, which considerably shortened the synthesis procedure. Their morphological features were characterized by high-resolution scanning transmission electron microscopy, UV-VIS spectroscopy, and small-angle X-ray scattering method. The toxicological properties of PVP-Se NPs were analyzed by colorimetric cell viability assay based on the reduction of a yellow tetrazolium salt (XTT) in mitochondria, a further assay based on the measurement of lactate dehydrogenase (LDH) release from damaged cell membrane, and finally by cell proliferation assay. The antifungal effect of PVP-Se NPs alone, or in a mixture of commercial fungicide containing 2% of boric acid, 2% alkylbenzyldimethylammonium chloride, and ∼0.9% ethanolamine in distilled water was tested on the brown-rot fungus Serpula lacrymans The fungus belongs to important basidiomycetes causing economically significant decay of wood, mainly in houses and particularly cold soil cellars. The average mass loss tests performed on spruce wood blocks after four months of inoculation with S. lacrymans showed an enhanced antifungal effect of the mixture of PVP-Se NPs with a commercial fungicide than a PVP-Se NPs or commercial fungicide alone, which proved their synergistic effect on inhibiting fungi growth. Finally, scanning electron microscopy analysis of outer and inner parts of wood blocks treated with our novel mixture was performed.
Návaznosti
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