J 2023

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes

MARTÍNEZ-GARCÍA, Esteban; Sofía FRAILE; Elena ALGAR; Tomás APARICIO; Elena VELÁZQUEZ et al.

Základní údaje

Originální název

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes

Autoři

MARTÍNEZ-GARCÍA, Esteban; Sofía FRAILE; Elena ALGAR; Tomás APARICIO; Elena VELÁZQUEZ; Belén CALLES; Huseyin TAS; Blas BLÁZQUEZ; Bruno MARTÍN; Clara PRIETO; Lucas SÁNCHEZ-SAMPEDRO; Morten H H NØRHOLM; Daniel C VOLKE; Nicolas T WIRTH; Pavel DVOŘÁK; Lorea ALEJALDRE; Lewis GROZINGER; Matthew CROWTHER; Angel GOÑI-MORENO; Pablo I NIKEL; Juan NOGALES a Víctor DE LORENZO

Vydání

Nucleic Acids Research, Oxford University Press, 2023, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20801 Environmental biotechnology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 16.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00134022

Organizační jednotka

Přírodovědecká fakulta

EID Scopus

Klíčová slova anglicky

Plasmid; database; standardization; synthetic biology

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 12. 7. 2023 11:31, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis and engineering of Gram-negative bacteria with a structure and nomenclature that follows a strict, fixed architecture of functional DNA segments. While the current update keeps the basic features of earlier versions, the platform has been upgraded not only with many more ready-to-use plasmids but also with features that expand the range of target species, harmonize DNA assembly methods and enable new applications. In particular, SEVA 4.0 includes (i) a sub-collection of plasmids for easing the composition of multiple DNA segments with MoClo/Golden Gate technology, (ii) vectors for Gram-positive bacteria and yeast and [iii] off-the-shelf constructs with built-in functionalities. A growing collection of plasmids that capture part of the standard—but not its entirety—has been compiled also into the DB and repository as a separate corpus (SEVAsib) because of its value as a resource for constructing and deploying phenotypes of interest. Maintenance and curation of the DB were accompanied by dedicated diffusion and communication channels that make the SEVA platform a popular resource for genetic analyses, genome editing and bioengineering of a large number of microorganisms.

Návaznosti

GA22-12505S, projekt VaV
Název: Syntetické konsorcium kmenů Pseudomonas putida pro biodegradaci a ko-utilizaci (hemi)celulózových polymerů
Investor: Grantová agentura ČR, Syntetické konsorcium kmenů Pseudomonas putida pro biodegradaci a ko-utilizaci (hemi)celulózových polymerů