Multilocus Sequence Genotype Heterogeneity in Streptococcus
uberis Isolated from Bovine Mastitis in the Czech Republic

J 2022

Multilocus Sequence Genotype Heterogeneity in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic

ZOUHAROVA, Monika; Katerina NEDBALCOVA; Natálie KRÁLOVÁ; Petr SLAMA; Katarina MATIASKOVA et al.

Základní údaje

Originální název

Multilocus Sequence Genotype Heterogeneity in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic

Autoři

ZOUHAROVA, Monika; Katerina NEDBALCOVA; Natálie KRÁLOVÁ; Petr SLAMA; Katarina MATIASKOVA a Jan MATIASOVIC

Vydání

Animals, MDPI, 2022, 2076-2615

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00127387

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

intramammary infection; MLST; antimicrobial resistance; virulence factors; cows

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 12. 2022 14:53, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The ubiquitous occurrence and high heterogeneity of Streptococcus uberis strains cause difficulties in the development and implementation of effective control strategies in dairy herds. In this study, S. uberis strains from 74 farms, obtained predominantly from subclinical, acute, and chronic recurrent mastitis, as well as from udder surface swabs and milk from healthy udders, were analysed for their genetic diversity using multilocus sequence typing (MLST). Isolates were tested for the presence of the genes encoding the virulence factors using polymerase chain reaction. Antibiotic susceptibility testing was performed using a microdilution assay including 14 antimicrobials. The virulence profiles and antimicrobial (AMR) profiles of the isolates were assembled and the overall heterogeneity was evaluated. Among the 124 isolates, 89 MLST genotypes, 7 different virulence profiles, and 12 AMR profiles were identified. The large number of different MLST allelic profiles in this study points to the high heterogeneity of strains in dairy herds in the Czech Republic. Isolates of a certain MLST genotype may possess a different set of virulence factor genes. We detected up to three different resistance profiles within a single MLST genotype. The results of our study showed that fully susceptible isolates coexisted with resistant or even multiresistant isolates in the same herd. Multiple genotypes within a herd were detected on many farms (up to seven MLST genotypes and four AMR profiles in one herd). This heterogenic population structure might suggest that environmental transmission is the predominant route of infection in herds in the Czech Republic.

Citovat

ZOUHAROVA, Monika; Katerina NEDBALCOVA; Natálie KRÁLOVÁ; Petr SLAMA; Katarina MATIASKOVA a Jan MATIASOVIC. Multilocus Sequence Genotype Heterogeneity in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic. Animals. MDPI, 2022, roč. 12, č. 18, s. 1-11. ISSN 2076-2615. Dostupné z: https://doi.org/10.3390/ani12182327.

@article{2238047,
   author = {Zouharova, Monika and Nedbalcova, Katerina and Králová, Natálie and Slama, Petr and Matiaskova, Katarina and Matiasovic, Jan},
   article_number = {18},
   doi = {https://doi.org/10.3390/ani12182327},
   keywords = {intramammary infection; MLST; antimicrobial resistance; virulence factors; cows},
   language = {eng},
   issn = {2076-2615},
   journal = {Animals},
   title = {Multilocus Sequence Genotype Heterogeneity in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic},
   url = {https://www.mdpi.com/2076-2615/12/18/2327},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 2238047
AU  - Zouharova, Monika - Nedbalcova, Katerina - Králová, Natálie - Slama, Petr - Matiaskova, Katarina - Matiasovic, Jan
PY  - 2022
TI  - Multilocus Sequence Genotype Heterogeneity in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic
JF  - Animals
VL  - 12
IS  - 18
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 20762615
KW  - intramammary infection
KW  - MLST
KW  - antimicrobial resistance
KW  - virulence factors
KW  - cows
UR  - https://www.mdpi.com/2076-2615/12/18/2327
N2  - The ubiquitous occurrence and high heterogeneity of Streptococcus uberis strains cause difficulties in the development and implementation of effective control strategies in dairy herds. In this study, S. uberis strains from 74 farms, obtained predominantly from subclinical, acute, and chronic recurrent mastitis, as well as from udder surface swabs and milk from healthy udders, were analysed for their genetic diversity using multilocus sequence typing (MLST). Isolates were tested for the presence of the genes encoding the virulence factors using polymerase chain reaction. Antibiotic susceptibility testing was performed using a microdilution assay including 14 antimicrobials. The virulence profiles and antimicrobial (AMR) profiles of the isolates were assembled and the overall heterogeneity was evaluated. Among the 124 isolates, 89 MLST genotypes, 7 different virulence profiles, and 12 AMR profiles were identified. The large number of different MLST allelic profiles in this study points to the high heterogeneity of strains in dairy herds in the Czech Republic. Isolates of a certain MLST genotype may possess a different set of virulence factor genes. We detected up to three different resistance profiles within a single MLST genotype. The results of our study showed that fully susceptible isolates coexisted with resistant or even multiresistant isolates in the same herd. Multiple genotypes within a herd were detected on many farms (up to seven MLST genotypes and four AMR profiles in one herd). This heterogenic population structure might suggest that environmental transmission is the predominant route of infection in herds in the Czech Republic.
ER  -

ZOUHAROVA, Monika; Katerina NEDBALCOVA; Natálie KRÁLOVÁ; Petr SLAMA; Katarina MATIASKOVA a Jan MATIASOVIC. Multilocus Sequence Genotype Heterogeneity in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic. \textit{Animals}. MDPI, 2022, roč.~12, č.~18, s.~1-11. ISSN~2076-2615. Dostupné z: https://doi.org/10.3390/ani12182327.

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