BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA
POLYMERASE AND RIBOSOME

a 2022

BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME

HIRIYUR NAGARAJ, Pradeep, Ahmed Adel I. H. HASSAN, Sylva BRABENCOVÁ, Lucie SLÁMOVÁ, Gabriel DEMO et. al.

Základní údaje

Originální název

BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME

Autoři

HIRIYUR NAGARAJ, Pradeep (356 Indie, domácí), Ahmed Adel I. H. HASSAN (818 Egypt, domácí), Sylva BRABENCOVÁ (203 Česká republika, domácí), Lucie SLÁMOVÁ (203 Česká republika, domácí) a Gabriel DEMO (703 Slovensko, garant, domácí)

Vydání

National Institute of Virology and Bacteriology Meeting, Kutná Hora, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/22:00127505

Organizační jednotka

Středoevropský technologický institut

ISSN

Klíčová slova anglicky

mRNA; RNAP; transcription-translation coupling complex

Štítky

rivok

Změněno: 19. 12. 2022 09:53, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Transcription of messenger RNAs and translation of proteins, mechanisms necessary for gene expression, can be physically coupled and coordinated in some bacterial species1,2. The interaction between the leading ribosome and RNA polymerase (RNAP) forms a transcription-translation coupling complex (CTT)2–4 modulated by transcription elongation factor NusG3,4. The leading ribosome also regulates the propagation of the RNAP during transcription1 and protects the RNAP from backtracking. Moreover, the transcription pausing and possible premature termination can be modulated by cis-acting RNA structures (riboswitches) which may play a crucial role in formation of coupled transcription-translation. Here, we investigate the coordination of translation initiation and elongation with RNAP pausing by forming CTT complexes in sucrose gradients. For translation initiation we use specific riboswitch to form and stabilize the initiation complex during early steps of CTT formation. For translation elongation we load the aminoacylated-tRNA into the CTT in the form of ternary complex.

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Citovat

HIRIYUR NAGARAJ, Pradeep, Ahmed Adel I. H. HASSAN, Sylva BRABENCOVÁ, Lucie SLÁMOVÁ a Gabriel DEMO. BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME. In National Institute of Virology and Bacteriology Meeting, Kutná Hora. 2022. ISSN 2336-7210.

@proceedings{2240854,
   author = {Hiriyur Nagaraj, Pradeep and Hassan, Ahmed Adel I. H. and Brabencová, Sylva and Slámová, Lucie and Demo, Gabriel},
   booktitle = {National Institute of Virology and Bacteriology Meeting, Kutná Hora},
   keywords = {mRNA; RNAP; transcription-translation coupling complex},
   language = {eng},
   title = {BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME},
   url = {http://www.ccsss.cz/index.php/ccsss/issue/view/37},
   year = {2022}
}

TY  - CONF
ID  - 2240854
AU  - Hiriyur Nagaraj, Pradeep - Hassan, Ahmed Adel I. H. - Brabencová, Sylva - Slámová, Lucie - Demo, Gabriel
PY  - 2022
TI  - BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME
KW  - mRNA
KW  - RNAP
KW  - transcription-translation coupling complex
UR  - http://www.ccsss.cz/index.php/ccsss/issue/view/37
N2  - Transcription of messenger RNAs and translation of proteins, mechanisms necessary for gene expression, can be physically coupled and coordinated in some bacterial species1,2. The interaction between the leading ribosome and RNA polymerase (RNAP) forms a transcription-translation coupling complex (CTT)2–4 modulated by transcription elongation factor NusG3,4. The leading ribosome also regulates the propagation of the RNAP during transcription1 and protects the RNAP from backtracking. Moreover, the transcription pausing and possible premature termination can be modulated by cis-acting RNA structures (riboswitches) which may play a crucial role in formation of coupled transcription-translation. Here, we investigate the coordination of translation initiation and elongation with RNAP pausing by forming CTT complexes in sucrose gradients. For translation initiation we use specific riboswitch to form and stabilize the initiation complex during early steps of CTT formation. For translation elongation we load the aminoacylated-tRNA into the CTT in the form of ternary complex.
ER  -

HIRIYUR NAGARAJ, Pradeep, Ahmed Adel I. H. HASSAN, Sylva BRABENCOVÁ, Lucie SLÁMOVÁ a Gabriel DEMO. BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME. In \textit{National Institute of Virology and Bacteriology Meeting, Kutná Hora}. 2022. ISSN~2336-7210.

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