Inhibition of Aryl Hydrocarbon Receptor (AhR) Expression
Disrupts Cell Proliferation and Alters Energy Metabolism and
Fatty Acid Synthesis in Colon Cancer Cells

J 2022

Inhibition of Aryl Hydrocarbon Receptor (AhR) Expression Disrupts Cell Proliferation and Alters Energy Metabolism and Fatty Acid Synthesis in Colon Cancer Cells

KARASOVÁ, Martina; Jiřina PROCHÁZKOVÁ; Zuzana TYLICHOVÁ; Radek FEDR; Miroslav CIGANEK et al.

Základní údaje

Originální název

Inhibition of Aryl Hydrocarbon Receptor (AhR) Expression Disrupts Cell Proliferation and Alters Energy Metabolism and Fatty Acid Synthesis in Colon Cancer Cells

Autoři

Vydání

Cancers, MDPI, 2022, 2072-6694

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00127670

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

colon cancer cells; AhR; metabolism; proliferation; fatty acid synthesis; Akt pathway

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 1. 2023 10:55, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The aryl hydrocarbon receptor (AhR) plays a wide range of physiological roles in cellular processes such as proliferation, migration or control of immune responses. Several studies have also indicated that AhR might contribute to the regulation of energy balance or cellular metabolism. We observed that the AhR is upregulated in tumor epithelial cells derived from colon cancer patients. Using wild-type and the corresponding AhR knockout (AhR KO) variants of human colon cancer cell lines HCT116 and HT-29, we analyzed possible role(s) of the AhR in cell proliferation and metabolism, with a focus on regulation of the synthesis of fatty acids (FAs). We observed a decreased proliferation rate in the AhR KO cells, which was accompanied with altered cell cycle progression, as well as a decreased ATP production. We also found reduced mRNA levels of key enzymes of the FA biosynthetic pathway in AhR KO colon cancer cells, in particular of stearoyl-CoA desaturase 1 (SCD1). The loss of AhR was also associated with reduced expression and/or activity of components of the PI3K/Akt pathway, which controls lipid metabolism, and other lipogenic transcriptional regulators, such as sterol regulatory element binding transcription factor 1 (SREBP1). Together, our data indicate that disruption of AhR activity in colon tumor cells may, likely in a cell-specific manner, limit their proliferation, which could be linked with a suppressive effect on their endogenous FA metabolism. More attention should be paid to potential mechanistic links between overexpressed AhR and colon tumor cell metabolism.

Citovat

KARASOVÁ, Martina; Jiřina PROCHÁZKOVÁ; Zuzana TYLICHOVÁ; Radek FEDR; Miroslav CIGANEK; Miroslav MACHALA; Zdeněk DVOŘÁK; Barbora VYHLÍDALOVÁ; Iveta ZŮVALOVÁ; Jiří EHRMANN; Jan BOUCHAL; Zdeněk ANDRYSÍK a Jan VONDRÁČEK. Inhibition of Aryl Hydrocarbon Receptor (AhR) Expression Disrupts Cell Proliferation and Alters Energy Metabolism and Fatty Acid Synthesis in Colon Cancer Cells. Cancers. MDPI, 2022, roč. 14, č. 17, s. 1-20. ISSN 2072-6694. Dostupné z: https://doi.org/10.3390/cancers14174245.

@article{2243453,
   author = {Karasová, Martina and Procházková, Jiřina and Tylichová, Zuzana and Fedr, Radek and Ciganek, Miroslav and Machala, Miroslav and Dvořák, Zdeněk and Vyhlídalová, Barbora and Zůvalová, Iveta and Ehrmann, Jiří and Bouchal, Jan and Andrysík, Zdeněk and Vondráček, Jan},
   article_number = {17},
   doi = {https://doi.org/10.3390/cancers14174245},
   keywords = {colon cancer cells; AhR; metabolism; proliferation; fatty acid synthesis; Akt pathway},
   language = {eng},
   issn = {2072-6694},
   journal = {Cancers},
   title = {Inhibition of Aryl Hydrocarbon Receptor (AhR) Expression Disrupts Cell Proliferation and Alters Energy Metabolism and Fatty Acid Synthesis in Colon Cancer Cells},
   url = {https://www.mdpi.com/2072-6694/14/17/4245},
   volume = {14},
   year = {2022}
}

TY  - JOUR
ID  - 2243453
AU  - Karasová, Martina - Procházková, Jiřina - Tylichová, Zuzana - Fedr, Radek - Ciganek, Miroslav - Machala, Miroslav - Dvořák, Zdeněk - Vyhlídalová, Barbora - Zůvalová, Iveta - Ehrmann, Jiří - Bouchal, Jan - Andrysík, Zdeněk - Vondráček, Jan
PY  - 2022
TI  - Inhibition of Aryl Hydrocarbon Receptor (AhR) Expression Disrupts Cell Proliferation and Alters Energy Metabolism and Fatty Acid Synthesis in Colon Cancer Cells
JF  - Cancers
VL  - 14
IS  - 17
SP  - 1-20
EP  - 1-20
PB  - MDPI
SN  - 20726694
KW  - colon cancer cells
KW  - AhR
KW  - metabolism
KW  - proliferation
KW  - fatty acid synthesis
KW  - Akt pathway
UR  - https://www.mdpi.com/2072-6694/14/17/4245
N2  - The aryl hydrocarbon receptor (AhR) plays a wide range of physiological roles in cellular processes such as proliferation, migration or control of immune responses. Several studies have also indicated that AhR might contribute to the regulation of energy balance or cellular metabolism. We observed that the AhR is upregulated in tumor epithelial cells derived from colon cancer patients. Using wild-type and the corresponding AhR knockout (AhR KO) variants of human colon cancer cell lines HCT116 and HT-29, we analyzed possible role(s) of the AhR in cell proliferation and metabolism, with a focus on regulation of the synthesis of fatty acids (FAs). We observed a decreased proliferation rate in the AhR KO cells, which was accompanied with altered cell cycle progression, as well as a decreased ATP production. We also found reduced mRNA levels of key enzymes of the FA biosynthetic pathway in AhR KO colon cancer cells, in particular of stearoyl-CoA desaturase 1 (SCD1). The loss of AhR was also associated with reduced expression and/or activity of components of the PI3K/Akt pathway, which controls lipid metabolism, and other lipogenic transcriptional regulators, such as sterol regulatory element binding transcription factor 1 (SREBP1). Together, our data indicate that disruption of AhR activity in colon tumor cells may, likely in a cell-specific manner, limit their proliferation, which could be linked with a suppressive effect on their endogenous FA metabolism. More attention should be paid to potential mechanistic links between overexpressed AhR and colon tumor cell metabolism.
ER  -

KARASOVÁ, Martina; Jiřina PROCHÁZKOVÁ; Zuzana TYLICHOVÁ; Radek FEDR; Miroslav CIGANEK; Miroslav MACHALA; Zdeněk DVOŘÁK; Barbora VYHLÍDALOVÁ; Iveta ZŮVALOVÁ; Jiří EHRMANN; Jan BOUCHAL; Zdeněk ANDRYSÍK a Jan VONDRÁČEK. Inhibition of Aryl Hydrocarbon Receptor (AhR) Expression Disrupts Cell Proliferation and Alters Energy Metabolism and Fatty Acid Synthesis in Colon Cancer Cells. \textit{Cancers}. MDPI, 2022, roč.~14, č.~17, s.~1-20. ISSN~2072-6694. Dostupné z: https://doi.org/10.3390/cancers14174245.

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