Enzyme-Catalyzed Polymerization Process: A Novel Approach to
the Preparation of Polyaniline Colloidal Dispersions with an
Immunomodulatory Effect

J 2022

Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect

JASENSKÁ, Daniela; Věra KAŠPÁRKOVÁ; Ondřej VAŠÍČEK; Lukáš MÜNSTER; Antonín MINAŘÍK et al.

Základní údaje

Originální název

Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect

Autoři

Vydání

Biomacromolecules, Washington, American Chemical Society, 2022, 1525-7797

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00127701

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Colloidal particles; Colloids; Plastics; Polymers; Toxicity

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 1. 2023 15:58, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

A green, nature-friendly synthesis of polyaniline colloidal particles based on enzyme-assisted oxidation of aniline with horseradish peroxidase and chitosan or poly(vinyl alcohol) as steric stabilizers was successfully employed. Physicochemical characterization revealed formation of particles containing the polyaniline emeraldine salt and demonstrated only a minor effect of polymer stabilizers on particle morphology. All tested colloidal particles showed in vitro antioxidation activity determined via scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. In vitro, they were able to reduce oxidative stress and inhibit the production of reactive oxygen species by neutrophils and inflammatory cytokines by macrophages. The anti-inflammatory effect observed was related to their antioxidant activity, especially in the case of neutrophils. The particles can thus be especially advantageous as active components of biomaterials modulating the early stages of inflammation. In addition to the immunomodulatory effect, the presence of intrinsically conducting polyaniline can impart cell-instructive properties to the particles. The approach to particle synthesis that we employed─an original one using environmentally friendly and biocompatible horseradish peroxidase─represents a smart way of preparing conducting particles with unique properties, which can be further modified by the stabilizers used.

Návaznosti

EF16_025/0007381, projekt VaV

Název: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou

Citovat

JASENSKÁ, Daniela; Věra KAŠPÁRKOVÁ; Ondřej VAŠÍČEK; Lukáš MÜNSTER; Antonín MINAŘÍK; Simona KÁČEROVÁ; Eva KORÁBKOVÁ; Lucie URBÁNKOVÁ; Jan VÍCHA; Zdenka CAPÁKOVÁ; Ermelinda FALLETA; Della Pina CRISTINA; Marián LEHOCKÝ; Kateřina SKOPALOVÁ a Petr HUMPOLÍČEK. Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect. Biomacromolecules. Washington: American Chemical Society, 2022, roč. 23, č. 8, s. 3359-3370. ISSN 1525-7797. Dostupné z: https://doi.org/10.1021/acs.biomac.2c00371.

@article{2243576,
   author = {Jasenská, Daniela and Kašpárková, Věra and Vašíček, Ondřej and Münster, Lukáš and Minařík, Antonín and Káčerová, Simona and Korábková, Eva and Urbánková, Lucie and Vícha, Jan and Capáková, Zdenka and Falleta, Ermelinda and Cristina, Della Pina and Lehocký, Marián and Skopalová, Kateřina and Humpolíček, Petr},
   article_location = {Washington},
   article_number = {8},
   doi = {https://doi.org/10.1021/acs.biomac.2c00371},
   keywords = {Colloidal particles; Colloids; Plastics; Polymers; Toxicity},
   language = {eng},
   issn = {1525-7797},
   journal = {Biomacromolecules},
   title = {Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect},
   url = {https://pubs.acs.org/doi/10.1021/acs.biomac.2c00371},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 2243576
AU  - Jasenská, Daniela - Kašpárková, Věra - Vašíček, Ondřej - Münster, Lukáš - Minařík, Antonín - Káčerová, Simona - Korábková, Eva - Urbánková, Lucie - Vícha, Jan - Capáková, Zdenka - Falleta, Ermelinda - Cristina, Della Pina - Lehocký, Marián - Skopalová, Kateřina - Humpolíček, Petr
PY  - 2022
TI  - Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect
JF  - Biomacromolecules
VL  - 23
IS  - 8
SP  - 3359-3370
EP  - 3359-3370
PB  - American Chemical Society
SN  - 15257797
KW  - Colloidal particles
KW  - Colloids
KW  - Plastics
KW  - Polymers
KW  - Toxicity
UR  - https://pubs.acs.org/doi/10.1021/acs.biomac.2c00371
N2  - A green, nature-friendly synthesis of polyaniline colloidal particles based on enzyme-assisted oxidation of aniline with horseradish peroxidase and chitosan or poly(vinyl alcohol) as steric stabilizers was successfully employed. Physicochemical characterization revealed formation of particles containing the polyaniline emeraldine salt and demonstrated only a minor effect of polymer stabilizers on particle morphology. All tested colloidal particles showed in vitro antioxidation activity determined via scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. In vitro, they were able to reduce oxidative stress and inhibit the production of reactive oxygen species by neutrophils and inflammatory cytokines by macrophages. The anti-inflammatory effect observed was related to their antioxidant activity, especially in the case of neutrophils. The particles can thus be especially advantageous as active components of biomaterials modulating the early stages of inflammation. In addition to the immunomodulatory effect, the presence of intrinsically conducting polyaniline can impart cell-instructive properties to the particles. The approach to particle synthesis that we employed─an original one using environmentally friendly and biocompatible horseradish peroxidase─represents a smart way of preparing conducting particles with unique properties, which can be further modified by the stabilizers used.
ER  -

JASENSKÁ, Daniela; Věra KAŠPÁRKOVÁ; Ondřej VAŠÍČEK; Lukáš MÜNSTER; Antonín MINAŘÍK; Simona KÁČEROVÁ; Eva KORÁBKOVÁ; Lucie URBÁNKOVÁ; Jan VÍCHA; Zdenka CAPÁKOVÁ; Ermelinda FALLETA; Della Pina CRISTINA; Marián LEHOCKÝ; Kateřina SKOPALOVÁ a Petr HUMPOLÍČEK. Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect. \textit{Biomacromolecules}. Washington: American Chemical Society, 2022, roč.~23, č.~8, s.~3359-3370. ISSN~1525-7797. Dostupné z: https://doi.org/10.1021/acs.biomac.2c00371.

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