Effects of salbutamol and ipratropium on human stem-cells
derived cardiomyocytes’ function

a 2022

Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function

ŠČUREK, Martin; Šimon KLIMOVIČ; Martin PEŠL; Jan PŘIBYL; Daniil KABANOV et al.

Základní údaje

Originální název

Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function

Autoři

ŠČUREK, Martin; Šimon KLIMOVIČ; Martin PEŠL; Jan PŘIBYL ; Daniil KABANOV; Deborah BECKEROVÁ; Markéta BÉBAROVÁ ; Vladimír ROTREKL a Kristián BRAT

Vydání

ERS International Congress 2022, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30203 Respiratory systems

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 24.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/22:00129720

Organizační jednotka

Lékařská fakulta

ISSN

DOI

https://doi.org/10.1183/13993003.congress-2022.560

UT WoS

000893392400426

Klíčová slova anglicky

human stem-cells derived cardiomyocytes’ function; salbutamol; ipratropium

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam

Změněno: 25. 10. 2024 16:02, Mgr. Natálie Hílek

Anotace

V originále

Introduction: Patients with obstructive pulmonary diseases are treated by beta-2-agonists, parasympatholytics, and methylxanthines for long periods. Chronic use of these drugs may result in impairment of the cardiovascular system. Direct effects of these bronchodilators on the function of human cardiomyocytes are described insufficiently. Aims: We aimed to investigate in vitro effects of salbutamol and ipratropium on human-induced pluripotent stem cell-derived cardiomyocyte (hiPS-CMs) cell clusters. We focused on their chronotropic and inotropic effects. Methods: The measurements of 3D clusters of hiPS-CMs, embryoid bodies (EBs), with spontaneous pacing and contraction abilities were performed using atomic force microscopy (AFM). AFM enables real-time monitoring of EBs’ beating frequency and is capable of measuring its contractility. We measured the effect of increasing concentrations of salbutamol and ipratropium on EBs. Results: We treated 25 EBs with salbutamol and compared results with the control group of 11 EBs. Similarly, we tested 11 EBs with ipratropium. We observed significant positive chronotropic and inotropic effects in higher concentrations of salbutamol with statistically significant elevation of contraction force for clinically relevant salbutamol concentration. Measurements with ipratropium did not reveal any changes in EBs’ electromechanical function. Conclusions: The effect of pulmonary drugs on the cardiovascular system can be reliably tested using a combination of AFM and hiPS-CMs. The clinically relevant concentration of salbutamol causes a significant elevation of human cardiomyocytes’ contractility and beating frequency.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

MUNI/A/1328/2021, interní kód MU

Název: Stanovení hladin bronchodilatancií v plazmě a efekt jejich kombinací na buněčné kultury kardiomyocytů

Investor: Masarykova univerzita, Stanovení hladin bronchodilatancií v plazmě a efekt jejich kombinací na buněčné kultury kardiomyocytů

NU20-06-00156, projekt VaV

Název: Vliv pneumologické medikace na funkce lidských kardiomyocytů.

Investor: Ministerstvo zdravotnictví ČR, Vliv pneumologické medikace na funkce lidských kardiomyocytů, Podprogram 1 - standardní

Citovat

ŠČUREK, Martin; Šimon KLIMOVIČ; Martin PEŠL; Jan PŘIBYL; Daniil KABANOV; Deborah BECKEROVÁ; Markéta BÉBAROVÁ; Vladimír ROTREKL a Kristián BRAT. Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function. In ERS International Congress 2022. 2022. ISSN 0903-1936. Dostupné z: https://doi.org/10.1183/13993003.congress-2022.560.

@proceedings{2243824,
   author = {Ščurek, Martin and Klimovič, Šimon and Pešl, Martin and Přibyl, Jan and Kabanov, Daniil and Beckerová, Deborah and Bébarová, Markéta and Rotrekl, Vladimír and Brat, Kristián},
   booktitle = {ERS International Congress 2022},
   doi = {https://doi.org/10.1183/13993003.congress-2022.560},
   keywords = {human stem-cells derived cardiomyocytes’ function; salbutamol; ipratropium},
   language = {eng},
   title = {Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function},
   url = {https://erj.ersjournals.com/content/60/suppl_66/560},
   year = {2022}
}

TY  - CONF
ID  - 2243824
AU  - Ščurek, Martin - Klimovič, Šimon - Pešl, Martin - Přibyl, Jan - Kabanov, Daniil - Beckerová, Deborah - Bébarová, Markéta - Rotrekl, Vladimír - Brat, Kristián
PY  - 2022
TI  - Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function
KW  - human stem-cells derived cardiomyocytes’ function
KW  - salbutamol
KW  - ipratropium
UR  - https://erj.ersjournals.com/content/60/suppl_66/560
N2  - Introduction: Patients with obstructive pulmonary diseases are treated by beta-2-agonists, parasympatholytics, and methylxanthines for long periods. Chronic use of these drugs may result in impairment of the cardiovascular system. Direct effects of these bronchodilators on the function of human cardiomyocytes are described insufficiently. Aims: We aimed to investigate in vitro effects of salbutamol and ipratropium on human-induced pluripotent stem cell-derived cardiomyocyte (hiPS-CMs) cell clusters. We focused on their chronotropic and inotropic effects. Methods: The measurements of 3D clusters of hiPS-CMs, embryoid bodies (EBs), with spontaneous pacing and contraction abilities were performed using atomic force microscopy (AFM). AFM enables real-time monitoring of EBs’ beating frequency and is capable of measuring its contractility. We measured the effect of increasing concentrations of salbutamol and ipratropium on EBs. Results: We treated 25 EBs with salbutamol and compared results with the control group of 11 EBs. Similarly, we tested 11 EBs with ipratropium. We observed significant positive chronotropic and inotropic effects in higher concentrations of salbutamol with statistically significant elevation of contraction force for clinically relevant salbutamol concentration. Measurements with ipratropium did not reveal any changes in EBs’ electromechanical function. Conclusions: The effect of pulmonary drugs on the cardiovascular system can be reliably tested using a combination of AFM and hiPS-CMs. The clinically relevant concentration of salbutamol causes a significant elevation of human cardiomyocytes’ contractility and beating frequency.
ER  -

ŠČUREK, Martin; Šimon KLIMOVIČ; Martin PEŠL; Jan PŘIBYL; Daniil KABANOV; Deborah BECKEROVÁ; Markéta BÉBAROVÁ; Vladimír ROTREKL a Kristián BRAT. Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function. In \textit{ERS International Congress 2022}. 2022. ISSN~0903-1936. Dostupné z: https://doi.org/10.1183/13993003.congress-2022.560.

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