Investigating the Origin and Evolution of Polyploid Trifolium
medium L. Karyotype by Comparative Cytogenomic Methods

J 2023

Investigating the Origin and Evolution of Polyploid Trifolium medium L. Karyotype by Comparative Cytogenomic Methods

LUKJANOVÁ, Eliška; Alžběta HANULÍKOVÁ a Jana ŘEPKOVÁ

Základní údaje

Originální název

Investigating the Origin and Evolution of Polyploid Trifolium medium L. Karyotype by Comparative Cytogenomic Methods

Autoři

LUKJANOVÁ, Eliška; Alžběta HANULÍKOVÁ a Jana ŘEPKOVÁ

Vydání

Plants, MDPI, 2023, 2223-7747

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00130145

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

clover; zigzag clover; fluorescent in situ hybridization; 5S rDNA; 26S rDNA; centromeric repeat; polyploidy

Štítky

14314010, 14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 2. 2023 12:01, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Trifolium medium L. is a wild polyploid relative of the agriculturally important red clover that possesses traits promising for breeding purposes. To date, T. medium also remains the only clover species with which agriculturally important red clover has successfully been hybridized. Even though allopolyploid origin has previously been suggested, little has in fact been known about the T. medium karyotype and its origin. We researched T. medium and related karyotypes using comparative cytogenomic methods, such as fluorescent in situ hybridization (FISH) and RepeatExplorer cluster analysis. The results indicate an exceptional karyotype diversity regarding numbers and mutual positions of 5S and 26S rDNA loci and centromeric repeats in populations of T. medium ecotypes and varieties. The observed variability among T. medium ecotypes and varieties suggests current karyotype instability that can be attributed to ever-ongoing battle between satellite DNA together with genomic changes and rearrangements enhanced by post-hybridization events. Comparative cytogenomic analyses of a T. medium hexaploid variety and diploid relatives revealed stable karyotypes with a possible case of chromosomal rearrangement. Moreover, the results provided evidence of T. medium having autopolyploid origin.

Návaznosti

MUNI/A/1325/2021, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10 (Akronym: MBG10)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10

Citovat

LUKJANOVÁ, Eliška; Alžběta HANULÍKOVÁ a Jana ŘEPKOVÁ. Investigating the Origin and Evolution of Polyploid Trifolium medium L. Karyotype by Comparative Cytogenomic Methods. Plants. MDPI, 2023, roč. 12, č. 2, s. 1-21. ISSN 2223-7747. Dostupné z: https://doi.org/10.3390/plants12020235.

@article{2243970,
   author = {Lukjanová, Eliška and Hanulíková, Alžběta and Řepková, Jana},
   article_number = {2},
   doi = {https://doi.org/10.3390/plants12020235},
   keywords = {clover; zigzag clover; fluorescent in situ hybridization; 5S rDNA; 26S rDNA; centromeric repeat; polyploidy},
   language = {eng},
   issn = {2223-7747},
   journal = {Plants},
   title = {Investigating the Origin and Evolution of Polyploid Trifolium medium L. Karyotype by Comparative Cytogenomic Methods},
   url = {https://www.mdpi.com/2223-7747/12/2/235},
   volume = {12},
   year = {2023}
}

TY  - JOUR
ID  - 2243970
AU  - Lukjanová, Eliška - Hanulíková, Alžběta - Řepková, Jana
PY  - 2023
TI  - Investigating the Origin and Evolution of Polyploid Trifolium medium L. Karyotype by Comparative Cytogenomic Methods
JF  - Plants
VL  - 12
IS  - 2
SP  - 1-21
EP  - 1-21
PB  - MDPI
SN  - 22237747
KW  - clover
KW  - zigzag clover
KW  - fluorescent in situ hybridization
KW  - 5S rDNA
KW  - 26S rDNA
KW  - centromeric repeat
KW  - polyploidy
UR  - https://www.mdpi.com/2223-7747/12/2/235
N2  - Trifolium medium L. is a wild polyploid relative of the agriculturally important red clover that possesses traits promising for breeding purposes. To date, T. medium also remains the only clover species with which agriculturally important red clover has successfully been hybridized. Even though allopolyploid origin has previously been suggested, little has in fact been known about the T. medium karyotype and its origin. We researched T. medium and related karyotypes using comparative cytogenomic methods, such as fluorescent in situ hybridization (FISH) and RepeatExplorer cluster analysis. The results indicate an exceptional karyotype diversity regarding numbers and mutual positions of 5S and 26S rDNA loci and centromeric repeats in populations of T. medium ecotypes and varieties. The observed variability among T. medium ecotypes and varieties suggests current karyotype instability that can be attributed to ever-ongoing battle between satellite DNA together with genomic changes and rearrangements enhanced by post-hybridization events. Comparative cytogenomic analyses of a T. medium hexaploid variety and diploid relatives revealed stable karyotypes with a possible case of chromosomal rearrangement. Moreover, the results provided evidence of T. medium having autopolyploid origin.
ER  -

LUKJANOVÁ, Eliška; Alžběta HANULÍKOVÁ a Jana ŘEPKOVÁ. Investigating the Origin and Evolution of Polyploid Trifolium medium L. Karyotype by Comparative Cytogenomic Methods. \textit{Plants}. MDPI, 2023, roč.~12, č.~2, s.~1-21. ISSN~2223-7747. Dostupné z: https://doi.org/10.3390/plants12020235.

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