Gene polymorphisms and serum levels of mannose-binding lectin
in Czech patients with recurrent aphthous stomatitis: A
case-control study

J 2023

Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study

IZAKOVIČOVÁ, Pavla; Simona SLEZÁKOVÁ; Zuzana ZAKOSTELSKA JIRASKOVA; Jana ŠÍSTKOVÁ; Natálie MLČŮCHOVÁ et. al.

Basic information

Original name

Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study

Authors

IZAKOVIČOVÁ, Pavla (203 Czech Republic, belonging to the institution); Simona SLEZÁKOVÁ (203 Czech Republic, belonging to the institution); Zuzana ZAKOSTELSKA JIRASKOVA (203 Czech Republic); Jana ŠÍSTKOVÁ (203 Czech Republic, belonging to the institution); Natálie MLČŮCHOVÁ (203 Czech Republic, belonging to the institution); Jirina BARTOVA (203 Czech Republic); Jitka PETANOVA (203 Czech Republic); Pavel KUKLÍNEK (203 Czech Republic, belonging to the institution); Antonín FASSMANN (203 Czech Republic, belonging to the institution); Ladislav DUŠEK (203 Czech Republic, belonging to the institution); Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, belonging to the institution)

Edition

JOURNAL OF ORAL PATHOLOGY & MEDICINE, HOBOKEN, WILEY, 2023, 0904-2512

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30208 Dentistry, oral surgery and medicine

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 2.700

RIV identification code

RIV/00216224:14110/23:00130185

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1111/jop.13385

UT WoS

000890131300001

EID Scopus

2-s2.0-85143410297

Keywords in English

haplogenotype; haplotype; MBL; polymorphism; recurrent aphthous stomatitis

Abstract

In the original language

Background: Recurrent aphthous stomatitis is one of the most prevalent oral mucosal immunological diseases. A recent case-control study in the Egyptian population suggested that single nucleotide polymorphism Gly54Asp (rs1800450) of the mannose-binding lectin 2 gene might affect the mannose-binding lectin serum level and recurrent aphthous stomatitis development. The aim of this study was to determine the distribution of six functional mannose-binding lectin 2 gene polymorphisms and analyse their role in recurrent aphthous stomatitis susceptibility in the Czech population. Methods: The study included 227 subjects; 137 healthy people and 90 patients with recurrent aphthous stomatitis. Six mannose-binding lectin 2 gene polymorphisms (rs11003125, rs7096206, rs7095891, rs5030737, rs1800450, rs1800451) were analysed by the SNaPshot assay method, mannose-binding lectin serum levels were determined by enzyme-linked immunosorbent assay (ELISA) method in a subgroup of subjects (N = 87). Results: No significant differences in mean of mannose-binding lectin serum levels between healthy controls and patients with recurrent aphthous stomatitis were observed (383 ng/ml +/- 249 standard deviation (SD) vs. 316 ng/ml +/- 177 SD in remission phase vs. 343 ng/ml +/- 254 SD in active phase; p > 0.05), also the allele and genotype frequencies of the studied mannose-binding lectin 2 polymorphisms did not differ significantly between the two groups (p > 0.05, odds ratio (OR): 0.75-1.23). Moreover, the distribution of mannose-binding lectin 2 haplotypes and haplogenotypes was similar in the healthy subjects and patients with recurrent aphthous stomatitis (p > 0.05, OR: 0.75-1.23). Conclusions: This study did not confirm the previously reported association of the mannose-binding lectin 2 Gly54Asp gene variant and low mannose-binding lectin serum level as the risk factors for susceptibility to recurrent aphthous stomatitis. In addition, no significant relationships between mannose-binding lectin 2 functional haplotypes or haplogenotypes and recurrent aphthous stomatitis were observed.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

MUNI/A/1398/2022, interní kód MU

Name: Etiopatogeneze, diagnostika, prevence a léčba nemocí dutiny ústní

Investor: Masaryk University, Etiopathogenesis, diagnosis, prevention and treatment of diseases of the oral cavity

MUNI/A/1445/2021, interní kód MU

Name: Nemoci dutiny ústní – výzkum jejich etiopatogeneze, diagnostiky a léčebných přístupů

Investor: Masaryk University

NV15-29336A, research and development project

Name: Změny imunologické reaktivity u pacientů s recidivujícími aftami

857560, interní kód MU
(CEP code: EF17_043/0009632)

Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)

Investor: European Union, Spreading excellence and widening participation

90121, large research infrastructures

Name: RECETOX RI

Cite

IZAKOVIČOVÁ, Pavla; Simona SLEZÁKOVÁ; Zuzana ZAKOSTELSKA JIRASKOVA; Jana ŠÍSTKOVÁ; Natálie MLČŮCHOVÁ; Jirina BARTOVA; Jitka PETANOVA; Pavel KUKLÍNEK; Antonín FASSMANN; Ladislav DUŠEK; Lydie IZAKOVIČOVÁ HOLLÁ and Petra BOŘILOVÁ LINHARTOVÁ. Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study. JOURNAL OF ORAL PATHOLOGY & MEDICINE. HOBOKEN: WILEY, 2023, vol. 52, No 1, p. 81-90. ISSN 0904-2512. Available from: https://dx.doi.org/10.1111/jop.13385.

@article{2247014,
   author = {Izakovičová, Pavla and Slezáková, Simona and Zakostelska Jiraskova, Zuzana and Šístková, Jana and Mlčůchová, Natálie and Bartova, Jirina and Petanova, Jitka and Kuklínek, Pavel and Fassmann, Antonín and Dušek, Ladislav and Izakovičová Hollá, Lydie and Bořilová Linhartová, Petra},
   article_location = {HOBOKEN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/jop.13385},
   keywords = {haplogenotype; haplotype; MBL; polymorphism; recurrent aphthous stomatitis},
   language = {eng},
   issn = {0904-2512},
   journal = {JOURNAL OF ORAL PATHOLOGY & MEDICINE},
   title = {Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/jop.13385},
   volume = {52},
   year = {2023}
}

TY  - JOUR
ID  - 2247014
AU  - Izakovičová, Pavla - Slezáková, Simona - Zakostelska Jiraskova, Zuzana - Šístková, Jana - Mlčůchová, Natálie - Bartova, Jirina - Petanova, Jitka - Kuklínek, Pavel - Fassmann, Antonín - Dušek, Ladislav - Izakovičová Hollá, Lydie - Bořilová Linhartová, Petra
PY  - 2023
TI  - Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study
JF  - JOURNAL OF ORAL PATHOLOGY & MEDICINE
VL  - 52
IS  - 1
SP  - 81-90
EP  - 81-90
PB  - WILEY
SN  - 09042512
KW  - haplogenotype
KW  - haplotype
KW  - MBL
KW  - polymorphism
KW  - recurrent aphthous stomatitis
UR  - https://onlinelibrary.wiley.com/doi/10.1111/jop.13385
N2  - Background: Recurrent aphthous stomatitis is one of the most prevalent oral mucosal immunological diseases. A recent case-control study in the Egyptian population suggested that single nucleotide polymorphism Gly54Asp (rs1800450) of the mannose-binding lectin 2 gene might affect the mannose-binding lectin serum level and recurrent aphthous stomatitis development. The aim of this study was to determine the distribution of six functional mannose-binding lectin 2 gene polymorphisms and analyse their role in recurrent aphthous stomatitis susceptibility in the Czech population. Methods: The study included 227 subjects; 137 healthy people and 90 patients with recurrent aphthous stomatitis. Six mannose-binding lectin 2 gene polymorphisms (rs11003125, rs7096206, rs7095891, rs5030737, rs1800450, rs1800451) were analysed by the SNaPshot assay method, mannose-binding lectin serum levels were determined by enzyme-linked immunosorbent assay (ELISA) method in a subgroup of subjects (N = 87). Results: No significant differences in mean of mannose-binding lectin serum levels between healthy controls and patients with recurrent aphthous stomatitis were observed (383 ng/ml +/- 249 standard deviation (SD) vs. 316 ng/ml +/- 177 SD in remission phase vs. 343 ng/ml +/- 254 SD in active phase; p > 0.05), also the allele and genotype frequencies of the studied mannose-binding lectin 2 polymorphisms did not differ significantly between the two groups (p > 0.05, odds ratio (OR): 0.75-1.23). Moreover, the distribution of mannose-binding lectin 2 haplotypes and haplogenotypes was similar in the healthy subjects and patients with recurrent aphthous stomatitis (p > 0.05, OR: 0.75-1.23). Conclusions: This study did not confirm the previously reported association of the mannose-binding lectin 2 Gly54Asp gene variant and low mannose-binding lectin serum level as the risk factors for susceptibility to recurrent aphthous stomatitis. In addition, no significant relationships between mannose-binding lectin 2 functional haplotypes or haplogenotypes and recurrent aphthous stomatitis were observed.
ER  -

IZAKOVIČOVÁ, Pavla; Simona SLEZÁKOVÁ; Zuzana ZAKOSTELSKA JIRASKOVA; Jana ŠÍSTKOVÁ; Natálie MLČŮCHOVÁ; Jirina BARTOVA; Jitka PETANOVA; Pavel KUKLÍNEK; Antonín FASSMANN; Ladislav DUŠEK; Lydie IZAKOVIČOVÁ HOLLÁ and Petra BOŘILOVÁ LINHARTOVÁ. Gene polymorphisms and serum levels of mannose-binding lectin in Czech patients with recurrent aphthous stomatitis: A case-control study. \textit{JOURNAL OF ORAL PATHOLOGY \&{} MEDICINE}. HOBOKEN: WILEY, 2023, vol.~52, No~1, p.~81-90. ISSN~0904-2512. Available from: https://dx.doi.org/10.1111/jop.13385.

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