Cytokinin modulates the metabolic network of sulfur and
glutathione

J 2022

Cytokinin modulates the metabolic network of sulfur and glutathione

PAVLŮ, Jaroslav; Pavel KERCHEV; Martin ČERNÝ; Jan NOVÁK; Miroslav BERKA et al.

Základní údaje

Originální název

Cytokinin modulates the metabolic network of sulfur and glutathione

Autoři

Vydání

Journal of Experimental Botany, Oxford University Press, 2022, 0022-0957

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00128153

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Arabidopsis thaliana; cytokinin; glutathione; nutrient homeostasis; sulfur metabolism; sulfur starvation; xenobiotic tolerance

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 7. 2023 10:04, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The phytohormone cytokinin is implicated in a range of growth, developmental, and defense processes. A growing body of evidence supports a crosstalk between cytokinin and nutrient signaling pathways, such as nitrate availability. Cytokinin signaling regulates sulfur-responsive gene expression, but the underlying molecular mechanisms and their impact on sulfur-containing metabolites have not been systematically explored. Using a combination of genetic and pharmacological tools, we investigated the interplay between cytokinin signaling and sulfur homeostasis. Exogenous cytokinin triggered sulfur starvation-like gene expression accompanied by a decrease in sulfate and glutathione content. This process was uncoupled from the activity of the major transcriptional regulator of sulfate starvation signaling SULFUR LIMITATION 1 and an important glutathione-degrading enzyme, γ-glutamyl cyclotransferase 2;1, expression of which was robustly up-regulated by cytokinin. Conversely, glutathione accumulation was observed in mutants lacking the cytokinin receptor ARABIDOPSIS HISTIDINE KINASE 3 and in cytokinin-deficient plants. Cytokinin-deficient plants displayed improved root growth upon exposure to glutathione-depleting chemicals which was attributed to a higher capacity to maintain glutathione levels. These results shed new light on the interplay between cytokinin signaling and sulfur homeostasis. They position cytokinin as an important modulator of sulfur uptake, assimilation, and remobilization in plant defense against xenobiotics and root growth.

Citovat

PAVLŮ, Jaroslav; Pavel KERCHEV; Martin ČERNÝ; Jan NOVÁK; Miroslav BERKA; Timothy O. JOBE; José Maria López RAMOS; Inigo SAIZ-FERNÁNDEZ; Aaron Michael RASHOTTE; Stanislav KOPRIVA a Břetislav BRZOBOHATÝ. Cytokinin modulates the metabolic network of sulfur and glutathione. Journal of Experimental Botany. Oxford University Press, 2022, roč. 73, č. 22, s. 7417-7433. ISSN 0022-0957. Dostupné z: https://doi.org/10.1093/jxb/erac391.

@article{2248000,
   author = {Pavlů, Jaroslav and Kerchev, Pavel and Černý, Martin and Novák, Jan and Berka, Miroslav and Jobe, Timothy O. and Ramos, José Maria López and SaizandFernández, Inigo and Rashotte, Aaron Michael and Kopriva, Stanislav and Brzobohatý, Břetislav},
   article_number = {22},
   doi = {https://doi.org/10.1093/jxb/erac391},
   keywords = {Arabidopsis thaliana; cytokinin; glutathione; nutrient homeostasis; sulfur metabolism; sulfur starvation; xenobiotic tolerance},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Cytokinin modulates the metabolic network of sulfur and glutathione},
   url = {https://academic.oup.com/jxb/article/73/22/7417/6760041},
   volume = {73},
   year = {2022}
}

TY  - JOUR
ID  - 2248000
AU  - Pavlů, Jaroslav - Kerchev, Pavel - Černý, Martin - Novák, Jan - Berka, Miroslav - Jobe, Timothy O. - Ramos, José Maria López - Saiz-Fernández, Inigo - Rashotte, Aaron Michael - Kopriva, Stanislav - Brzobohatý, Břetislav
PY  - 2022
TI  - Cytokinin modulates the metabolic network of sulfur and glutathione
JF  - Journal of Experimental Botany
VL  - 73
IS  - 22
SP  - 7417-7433
EP  - 7417-7433
PB  - Oxford University Press
SN  - 00220957
KW  - Arabidopsis thaliana
KW  - cytokinin
KW  - glutathione
KW  - nutrient homeostasis
KW  - sulfur metabolism
KW  - sulfur starvation
KW  - xenobiotic tolerance
UR  - https://academic.oup.com/jxb/article/73/22/7417/6760041
N2  - The phytohormone cytokinin is implicated in a range of growth, developmental, and defense processes. A growing body of evidence supports a crosstalk between cytokinin and nutrient signaling pathways, such as nitrate availability. Cytokinin signaling regulates sulfur-responsive gene expression, but the underlying molecular mechanisms and their impact on sulfur-containing metabolites have not been systematically explored. Using a combination of genetic and pharmacological tools, we investigated the interplay between cytokinin signaling and sulfur homeostasis. Exogenous cytokinin triggered sulfur starvation-like gene expression accompanied by a decrease in sulfate and glutathione content. This process was uncoupled from the activity of the major transcriptional regulator of sulfate starvation signaling SULFUR LIMITATION 1 and an important glutathione-degrading enzyme, γ-glutamyl cyclotransferase 2;1, expression of which was robustly up-regulated by cytokinin. Conversely, glutathione accumulation was observed in mutants lacking the cytokinin receptor ARABIDOPSIS HISTIDINE KINASE 3 and in cytokinin-deficient plants. Cytokinin-deficient plants displayed improved root growth upon exposure to glutathione-depleting chemicals which was attributed to a higher capacity to maintain glutathione levels. These results shed new light on the interplay between cytokinin signaling and sulfur homeostasis. They position cytokinin as an important modulator of sulfur uptake, assimilation, and remobilization in plant defense against xenobiotics and root growth.
ER  -

PAVLŮ, Jaroslav; Pavel KERCHEV; Martin ČERNÝ; Jan NOVÁK; Miroslav BERKA; Timothy O. JOBE; José Maria López RAMOS; Inigo SAIZ-FERNÁNDEZ; Aaron Michael RASHOTTE; Stanislav KOPRIVA a Břetislav BRZOBOHATÝ. Cytokinin modulates the metabolic network of sulfur and glutathione. \textit{Journal of Experimental Botany}. Oxford University Press, 2022, roč.~73, č.~22, s.~7417-7433. ISSN~0022-0957. Dostupné z: https://doi.org/10.1093/jxb/erac391.

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