Classification of clinical Cutibacterium acnes isolates at
phylotype level by capillary electrophoretic methods in
roughened fused silica capillary

J 2022

Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary

HORKA, Marie; Filip RŮŽIČKA; Pavel KARASEK; Jiri SALPLACHTA; Alena SIVÁKOVÁ et al.

Základní údaje

Originální název

Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary

Autoři

HORKA, Marie; Filip RŮŽIČKA ; Pavel KARASEK; Jiri SALPLACHTA; Alena SIVÁKOVÁ a Michal ROTH

Vydání

Talanta, AMSTERDAM, Elsevier, 2022, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.100

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/22:00128305

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Capillary electrophoresis; Classification; Cutibacterium acnes; Fused silica capillary; Phylotype; Supercritical water

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 1. 2023 08:44, Mgr. Tereza Miškechová

Anotace

V originále

Despite being commensal bacterium involved in the maintenance of healthy skin, Cutibacterium acnes is also associated with inflammatory diseases. Since inflammatory and immunogenic properties vary between C. acnes phylotypes, reliable classification of clinical C. acnes isolates is important for determining their pathogenicity. Combination of optimized separation methods, polymer-enhanced transient isotachophoresis and sweeping of the charged bacterial cells in micellar electrokinetic chromatography in the roughened fused silica capillary, was used for the separation of twenty clinical C. acnes isolates. Their correct classification into the individual phylotypes was achieved in 20 min at laboratory temperature. In addition, decrease in the separation temperature to 15 degrees C led to the separation of the individual isolates of some phylotypes. Relative standard deviations of migration times of both intra- and inter-day analyses did not exceed 1.7%. Linearity of the proposed method in the concentration range from 5 x 10(5) to 1 x 10(7) cells mL(-1) was characterized by the coefficient of determination R-2 = 0.9985. Limit of detection of 5 x 10(5) cells mL(-1) (50 cells in 100 nL of the injected sample) was determined for all the examined bacteria.

Citovat

HORKA, Marie; Filip RŮŽIČKA; Pavel KARASEK; Jiri SALPLACHTA; Alena SIVÁKOVÁ a Michal ROTH. Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary. Talanta. AMSTERDAM: Elsevier, 2022, roč. 247, September 2022, s. 1-7. ISSN 0039-9140. Dostupné z: https://doi.org/10.1016/j.talanta.2022.123565.

@article{2249998,
   author = {Horka, Marie and Růžička, Filip and Karasek, Pavel and Salplachta, Jiri and Siváková, Alena and Roth, Michal},
   article_location = {AMSTERDAM},
   article_number = {September 2022},
   doi = {https://doi.org/10.1016/j.talanta.2022.123565},
   keywords = {Capillary electrophoresis; Classification; Cutibacterium acnes; Fused silica capillary; Phylotype; Supercritical water},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914022003617?via%3Dihub},
   volume = {247},
   year = {2022}
}

TY  - JOUR
ID  - 2249998
AU  - Horka, Marie - Růžička, Filip - Karasek, Pavel - Salplachta, Jiri - Siváková, Alena - Roth, Michal
PY  - 2022
TI  - Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary
JF  - Talanta
VL  - 247
IS  - September 2022
SP  - 1-7
EP  - 1-7
PB  - Elsevier
SN  - 00399140
KW  - Capillary electrophoresis
KW  - Classification
KW  - Cutibacterium acnes
KW  - Fused silica capillary
KW  - Phylotype
KW  - Supercritical water
UR  - https://www.sciencedirect.com/science/article/pii/S0039914022003617?via%3Dihub
N2  - Despite being commensal bacterium involved in the maintenance of healthy skin, Cutibacterium acnes is also associated with inflammatory diseases. Since inflammatory and immunogenic properties vary between C. acnes phylotypes, reliable classification of clinical C. acnes isolates is important for determining their pathogenicity. Combination of optimized separation methods, polymer-enhanced transient isotachophoresis and sweeping of the charged bacterial cells in micellar electrokinetic chromatography in the roughened fused silica capillary, was used for the separation of twenty clinical C. acnes isolates. Their correct classification into the individual phylotypes was achieved in 20 min at laboratory temperature. In addition, decrease in the separation temperature to 15 degrees C led to the separation of the individual isolates of some phylotypes. Relative standard deviations of migration times of both intra- and inter-day analyses did not exceed 1.7%. Linearity of the proposed method in the concentration range from 5 x 10(5) to 1 x 10(7) cells mL(-1) was characterized by the coefficient of determination R-2 = 0.9985. Limit of detection of 5 x 10(5) cells mL(-1) (50 cells in 100 nL of the injected sample) was determined for all the examined bacteria.
ER  -

HORKA, Marie; Filip RŮŽIČKA; Pavel KARASEK; Jiri SALPLACHTA; Alena SIVÁKOVÁ a Michal ROTH. Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary. \textit{Talanta}. AMSTERDAM: Elsevier, 2022, roč.~247, September 2022, s.~1-7. ISSN~0039-9140. Dostupné z: https://doi.org/10.1016/j.talanta.2022.123565.

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