High frequency of Nichols-like strains and increased levels of
macrolide resistance in Treponema pallidum in clinical samples
from Buenos Aires, Argentina

J 2022

High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina

MORANDO, Nicolas; Eliška VRBOVÁ; Asunta MELGAR; Roberto Daniel RABINOVICH; David ŠMAJS et al.

Základní údaje

Originální název

High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina

Autoři

MORANDO, Nicolas; Eliška VRBOVÁ; Asunta MELGAR; Roberto Daniel RABINOVICH; David ŠMAJS a Maria A PANDO

Vydání

Nature Scientific Reports, Berlin, NATURE RESEARCH, 2022, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/22:00128324

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Nichols-like strains; Treponema pallidum; macrolide resistance

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 1. 2023 12:28, Mgr. Tereza Miškechová

Anotace

V originále

Globally, 94% of Treponema pallidum subsp. pallidum (TPA) clinical strains belong to the SS14-like group and 6% to the Nichols-like group, with a prevalence of macrolide resistance of 90%. Our goal was to determine whether local TPA strain distribution and macrolide resistance frequency have changed significantly since our last report, which revealed that Buenos Aires had a high frequency of Nichols-like strains (27%) and low levels of macrolide resistance (14%). Swab samples from patients with suspected syphilis were collected during 2015-2019 and loci TP0136, TP0548, TP0705 were sequenced in order to perform multilocus sequence typing. Strains were classified as Nichols-like or SS14-like. The presence of macrolide resistance-associated mutations was determined by examination of the 23S rDNA gene sequence. Of 46 typeable samples, 37% were classified as Nichols-like and 63% as SS14-like. Macrolide resistance prevalence was 45.7%. Seven allelic profiles were found, five were SS14-like and two were Nichols-like. The frequency of Nichols-like strains increased between studies (26.8% vs. 37%, p = 0.36). A dramatic increase was found in the frequency of macrolide resistant strains between studies (14.3% vs. 45.7%, p = 0.005). Our results are in agreement with international trends and underscore the need to pursue further TPA molecular typing studies in South America.

Citovat

MORANDO, Nicolas; Eliška VRBOVÁ; Asunta MELGAR; Roberto Daniel RABINOVICH; David ŠMAJS a Maria A PANDO. High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina. Nature Scientific Reports. Berlin: NATURE RESEARCH, 2022, roč. 12, č. 1, s. 1-10. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-022-20410-5.

@article{2250049,
   author = {Morando, Nicolas and Vrbová, Eliška and Melgar, Asunta and Rabinovich, Roberto Daniel and Šmajs, David and Pando, Maria A},
   article_location = {Berlin},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41598-022-20410-5},
   keywords = {Nichols-like strains; Treponema pallidum; macrolide resistance},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina},
   url = {https://www.nature.com/articles/s41598-022-20410-5},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 2250049
AU  - Morando, Nicolas - Vrbová, Eliška - Melgar, Asunta - Rabinovich, Roberto Daniel - Šmajs, David - Pando, Maria A
PY  - 2022
TI  - High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina
JF  - Nature Scientific Reports
VL  - 12
IS  - 1
SP  - 1-10
EP  - 1-10
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Nichols-like strains
KW  - Treponema pallidum
KW  - macrolide resistance
UR  - https://www.nature.com/articles/s41598-022-20410-5
N2  - Globally, 94% of Treponema pallidum subsp. pallidum (TPA) clinical strains belong to the SS14-like group and 6% to the Nichols-like group, with a prevalence of macrolide resistance of 90%. Our goal was to determine whether local TPA strain distribution and macrolide resistance frequency have changed significantly since our last report, which revealed that Buenos Aires had a high frequency of Nichols-like strains (27%) and low levels of macrolide resistance (14%). Swab samples from patients with suspected syphilis were collected during 2015-2019 and loci TP0136, TP0548, TP0705 were sequenced in order to perform multilocus sequence typing. Strains were classified as Nichols-like or SS14-like. The presence of macrolide resistance-associated mutations was determined by examination of the 23S rDNA gene sequence. Of 46 typeable samples, 37% were classified as Nichols-like and 63% as SS14-like. Macrolide resistance prevalence was 45.7%. Seven allelic profiles were found, five were SS14-like and two were Nichols-like. The frequency of Nichols-like strains increased between studies (26.8% vs. 37%, p = 0.36). A dramatic increase was found in the frequency of macrolide resistant strains between studies (14.3% vs. 45.7%, p = 0.005). Our results are in agreement with international trends and underscore the need to pursue further TPA molecular typing studies in South America.
ER  -

MORANDO, Nicolas; Eliška VRBOVÁ; Asunta MELGAR; Roberto Daniel RABINOVICH; David ŠMAJS a Maria A PANDO. High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina. \textit{Nature Scientific Reports}. Berlin: NATURE RESEARCH, 2022, roč.~12, č.~1, s.~1-10. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-022-20410-5.

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