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@proceedings{2250086,
author = {Crha, Tomáš and Pazourek, Jiří},
booktitle = {50th Conference SYNTHESIS AND ANALYSIS OF DRUGS},
keywords = {HILIC; ELSD; lactose; sugars; milk; infant formula},
language = {eng},
isbn = {978-80-280-0110-0},
title = {DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL INFANT’S FORMULAS BY HPLC},
year = {2022}
}
TY - CONF
ID - 2250086
AU - Crha, Tomáš - Pazourek, Jiří
PY - 2022
TI - DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL INFANT’S FORMULAS BY HPLC
SN - 9788028001100
KW - HILIC
KW - ELSD
KW - lactose
KW - sugars
KW - milk
KW - infant formula
N2 - HPLC (high performance liquid chromatography) in the HILIC mode (hydrophilic interaction chromatography) is currently very often used in pharmacy for the separation and determination of polar analytes on polar stationary phases (in our case saccharides on the polyol stationary phase). However, since carbohydrates do not contain a suitable chromophore in their structure, a conventional UV detector is unusable for the determination, a refractometric detector would require isocratic elution, and therefore an evaporative light scattering detector (ELSD) can be used to advantage. An HPLC-ELSD method was developed for milk sugar profiling. Lactose was determined and quantified in selected commercially available infant formula samples.
ER -
CRHA, Tomáš a Jiří PAZOUREK. DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL INFANT’S FORMULAS BY HPLC. In \textit{50th Conference SYNTHESIS AND ANALYSIS OF DRUGS}. 2022. ISBN~978-80-280-0110-0.
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