Genic and chromosomal components of Prdm9-driven hybrid male
sterility in mice (Mus musculus)

J 2022

Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus)

VALISKOVA, Barbora; Sona GREGOROVA; Diana LUSTYK; Petr ŠIMEČEK; Petr JANSA et al.

Základní údaje

Originální název

Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus)

Autoři

VALISKOVA, Barbora; Sona GREGOROVA; Diana LUSTYK; Petr ŠIMEČEK; Petr JANSA a Jiri FOREJT

Vydání

Genetics, BETHESDA (USA), GENETICS SOCIETY AMERICA, 2022, 0016-6731

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.300

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/22:00128478

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

spermatogenesis; meiosis; homologous synapsis; SYCP3; HORMAD2; synaptonemal complex

Štítky

CF BIOIT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 10. 2024 12:40, Ing. Marie Švancarová

Anotace

V originále

Hybrid sterility contributes to speciation by preventing gene flow between related taxa. Prdm9, the first and only hybrid male sterility gene known in vertebrates, predetermines the sites of recombination between homologous chromosomes and their synapsis in early meiotic prophase. The asymmetric binding of PRDM9 to heterosubspecific homologs of Mus musculus musculus x Mus musculus domesticus F1 hybrids and increase of PRDM9-independent DNA double-strand break hotspots results indificult- to- repair double-strand breaks, incomplete synapsis of homologous chromosomes, and meiotic arrest at the first meiotic prophase. Here, we show that Prdm9 behaves as a major hybrid male sterility gene in mice outside the Mus musculus musculus x Mus musculus domesticus F1 hybrids, in the genomes composed of Mus musculus castaneus and Mus musculus musculus chromosomes segregating on the Mus musculus domesticus background. The Prdm9(cst/dom2) (castaneus/domesticus) allelic combination secures meiotic synapsis, testes weight, and sperm count within physiological limits, while the Prdm9(msc1/dom2) (musculus/domesticus) males show a range of fertility impairment. Out of 5 quantitative trait loci contributing to the Prdm9(msc1/dom2)-related infertility, 4 control either meiotic synapsis or fertility phenotypes and 1 controls both, synapsis, and fertility. Whole-genome genotyping of individual chromosomes showed preferential involvement of nonrecombinant musculus chromosomes in asynapsis in accordance with the chromosomal character of hybrid male sterility. Moreover, we show that the overall asynapsis rate can be estimated solely from the genotype of individual males by scoring the effect of nonrecombinant musculus chromosomes. Prdm9-controlled hybrid male sterility represents an example of genetic architecture of hybrid male sterility consisting of genic and chromosomal components.

Návaznosti

90267, velká výzkumná infrastruktura

Název: NCMG III

Citovat

VALISKOVA, Barbora; Sona GREGOROVA; Diana LUSTYK; Petr ŠIMEČEK; Petr JANSA a Jiri FOREJT. Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus). Genetics. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2022, roč. 222, č. 1, s. 1-14. ISSN 0016-6731. Dostupné z: https://doi.org/10.1093/genetics/iyac116.

@article{2251936,
   author = {Valiskova, Barbora and Gregorova, Sona and Lustyk, Diana and Šimeček, Petr and Jansa, Petr and Forejt, Jiri},
   article_location = {BETHESDA (USA)},
   article_number = {1},
   doi = {https://doi.org/10.1093/genetics/iyac116},
   keywords = {spermatogenesis; meiosis; homologous synapsis; SYCP3; HORMAD2; synaptonemal complex},
   language = {eng},
   issn = {0016-6731},
   journal = {Genetics},
   title = {Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus)},
   url = {https://academic.oup.com/genetics/article/222/1/iyac116/6655690?login=true},
   volume = {222},
   year = {2022}
}

TY  - JOUR
ID  - 2251936
AU  - Valiskova, Barbora - Gregorova, Sona - Lustyk, Diana - Šimeček, Petr - Jansa, Petr - Forejt, Jiri
PY  - 2022
TI  - Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus)
JF  - Genetics
VL  - 222
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - GENETICS SOCIETY AMERICA
SN  - 00166731
KW  - spermatogenesis
KW  - meiosis
KW  - homologous synapsis
KW  - SYCP3
KW  - HORMAD2
KW  - synaptonemal complex
UR  - https://academic.oup.com/genetics/article/222/1/iyac116/6655690?login=true
N2  - Hybrid sterility contributes to speciation by preventing gene flow between related taxa. Prdm9, the first and only hybrid male sterility gene known in vertebrates, predetermines the sites of recombination between homologous chromosomes and their synapsis in early meiotic prophase. The asymmetric binding of PRDM9 to heterosubspecific homologs of Mus musculus musculus x Mus musculus domesticus F1 hybrids and increase of PRDM9-independent DNA double-strand break hotspots results indificult- to- repair double-strand breaks, incomplete synapsis of homologous chromosomes, and meiotic arrest at the first meiotic prophase. Here, we show that Prdm9 behaves as a major hybrid male sterility gene in mice outside the Mus musculus musculus x Mus musculus domesticus F1 hybrids, in the genomes composed of Mus musculus castaneus and Mus musculus musculus chromosomes segregating on the Mus musculus domesticus background. The Prdm9(cst/dom2) (castaneus/domesticus) allelic combination secures meiotic synapsis, testes weight, and sperm count within physiological limits, while the Prdm9(msc1/dom2) (musculus/domesticus) males show a range of fertility impairment. Out of 5 quantitative trait loci contributing to the Prdm9(msc1/dom2)-related infertility, 4 control either meiotic synapsis or fertility phenotypes and 1 controls both, synapsis, and fertility. Whole-genome genotyping of individual chromosomes showed preferential involvement of nonrecombinant musculus chromosomes in asynapsis in accordance with the chromosomal character of hybrid male sterility. Moreover, we show that the overall asynapsis rate can be estimated solely from the genotype of individual males by scoring the effect of nonrecombinant musculus chromosomes. Prdm9-controlled hybrid male sterility represents an example of genetic architecture of hybrid male sterility consisting of genic and chromosomal components.
ER  -

VALISKOVA, Barbora; Sona GREGOROVA; Diana LUSTYK; Petr ŠIMEČEK; Petr JANSA a Jiri FOREJT. Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus). \textit{Genetics}. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2022, roč.~222, č.~1, s.~1-14. ISSN~0016-6731. Dostupné z: https://doi.org/10.1093/genetics/iyac116.

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