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@article{2259397, author = {Krausova, Katerina and Charousova, Marketa and Kratochvil, Zdenek and Takacsova, Paulina and Tesarova, Barbora and Sivak, Ladislav and Kudličková Pešková, Marie and Sukupova, Martina and Zivotska, Hana and Makovicky, Pavol and Yamashita, Ichiro and Okamoto, Naofumi and Hynek, David and Haddad, Yazan and Pekařík, Vladimír and Rex, Simona and Heger, Zbynek}, article_location = {Amsterdam}, article_number = {AUG}, doi = {http://dx.doi.org/10.1016/j.apmt.2022.101535}, keywords = {Archaeal ferritin; Continuous multimodal nanoparticle size; analysis; Mammalian ferritin; Native PAGE; Protein corona}, language = {eng}, issn = {2352-9407}, journal = {Applied Materials Today}, title = {Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition}, url = {https://www.sciencedirect.com/science/article/pii/S2352940722001706?via%3Dihub}, volume = {28}, year = {2022} }
TY - JOUR ID - 2259397 AU - Krausova, Katerina - Charousova, Marketa - Kratochvil, Zdenek - Takacsova, Paulina - Tesarova, Barbora - Sivak, Ladislav - Kudličková Pešková, Marie - Sukupova, Martina - Zivotska, Hana - Makovicky, Pavol - Yamashita, Ichiro - Okamoto, Naofumi - Hynek, David - Haddad, Yazan - Pekařík, Vladimír - Rex, Simona - Heger, Zbynek PY - 2022 TI - Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition JF - Applied Materials Today VL - 28 IS - AUG SP - 101535 EP - 101535 PB - Elsevier SN - 23529407 KW - Archaeal ferritin KW - Continuous multimodal nanoparticle size KW - analysis KW - Mammalian ferritin KW - Native PAGE KW - Protein corona UR - https://www.sciencedirect.com/science/article/pii/S2352940722001706?via%3Dihub N2 - Understanding the reversible reassembly properties of ferritins (FRTs) is crucial for enabling their applicability in nanomedicine. These properties include drug loading capabilities and also subsequent payload release in desired site-of-action. Thus, the presented study is focused on understanding the disassembly of recombinantly produced FRTs of varying origin and subunit composition, i.e. equine FRT composed of 22 L- and 2 H-subunits (EcaLH) or 24 L-subunits (EcaL), human FRT composed of 24 H-subunits (HsaH) and archaeal Pyrococcus furiosus FRT (Pfu). Disassembly was distinctly influenced by pH and ionic strength. Except for Pfu, the disassembly kinetics in acidic pH is rapid upon reaching an innate threshold, reaching the final state within minutes. Disassembly kinetics in basic pH is slower. Pfu is partially disassembled within the entire pH range. While equine FRT disassembles into free subunits, HsaH disassembles into subunit clusters. EcaL and Pfu form large aggregates during disassembly in mildly acidic pH, although basic pH causes partial disassembly without aggregation, suggesting usability for basic pH-triggered drug loading. We show that in human serum/plasma, FRTs readily form protein corona, hampering their uptake. Interestingly, we found out that archaeal Pfu likely exhibits similar receptor affinity as mammalian FRTs. Further, in vivo toxicity and biodistribution study of a single dose of FRTs demonstrated very low toxicity of FRTs and their preferential liver/kidney bioaccumulation. ER -
KRAUSOVA, Katerina, Marketa CHAROUSOVA, Zdenek KRATOCHVIL, Paulina TAKACSOVA, Barbora TESAROVA, Ladislav SIVAK, Marie KUDLIČKOVÁ PEŠKOVÁ, Martina SUKUPOVA, Hana ZIVOTSKA, Pavol MAKOVICKY, Ichiro YAMASHITA, Naofumi OKAMOTO, David HYNEK, Yazan HADDAD, Vladimír PEKAŘÍK, Simona REX a Zbynek HEGER. Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition. \textit{Applied Materials Today}. Amsterdam: Elsevier, 2022, roč.~28, AUG, s.~101535-101546. ISSN~2352-9407. Dostupné z: https://dx.doi.org/10.1016/j.apmt.2022.101535.
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