FARCI, Domenica, Patrycja HANIEWICZ and Dario PIANO. The structured organization of Deinococcus radiodurans' cell envelope. Proceedings of the National Academy of Sciences of the United States of America. 2022, vol. 119, No 45, p. 1-8. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.2209111119.
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Basic information
Original name The structured organization of Deinococcus radiodurans' cell envelope
Authors FARCI, Domenica, Patrycja HANIEWICZ and Dario PIANO.
Edition Proceedings of the National Academy of Sciences of the United States of America, 2022, 0027-8424.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 11.100
RIV identification code RIV/00216224:14740/22:00128752
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1073/pnas.2209111119
UT WoS 000907643500039
Keywords in English cryo-electron crystallography; cryo-electron tomography; S-layer; Type IV piliation system; SDBC
Tags CF CRYO, ne MU, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 28/2/2023 14:16.
Abstract
Surface layers (S-layers) are highly ordered coats of proteins localized on the cell surface of many bacterial species. In these structures, one or more proteins form elementary units that self-assemble into a crystalline monolayer tiling the entire cell surface. Here, the cell envelope of the radiation-resistant bacterium Deinococcus radiodurans was studied by cryo-electron microscopy, finding the crystalline regularity of the S-layer extended into the layers below (outer membrane, periplasm, and inner membrane). The cell envelope appears to be highly packed and resulting from a three-dimensional crystalline distribution of protein complexes organized in close continuity yet allowing a certain degree of free space. The presented results suggest how S-layers, at least in some species, are mesoscale assemblies behaving as structural and functional scaffolds essential for the entire cell envelope.
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