Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for
the Solubilization of Membrane Proteins: The Influence of
Composition and Molecular Weight

J 2022

Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight

JANATA, Miroslav, Eva CADOVA, Pavla ANGELISOVA, Tatsiana CHARNAVETS, Vaclav HOREJSI et. al.

Základní údaje

Originální název

Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight

Autoři

JANATA, Miroslav, Eva CADOVA, Pavla ANGELISOVA, Tatsiana CHARNAVETS, Vaclav HOREJSI a Vladimir RAUS

Vydání

Macromolecular Bioscience, WEINHEIM, Wiley-VCH, 2022, 1616-5187

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600

Kód RIV

RIV/00216224:14740/22:00128767

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/mabi.202200284

UT WoS

000842344300001

Klíčová slova anglicky

amphiphilic copolymers; isolation; membrane proteins; screening; solubilization

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 2. 2023 15:33, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Low-molecular weight (MW) amphiphilic copolymers have been recently introduced as a powerful tool for the detergent-free isolation of cell membrane proteins. Herein, a screening approach is used to identify a new copolymer type for this application. Via a two-step ATRP/acidolysis procedure, a 3 x 3 matrix of well-defined poly[(butyl methacrylate)-co-(methacrylic acid)] copolymers (denoted BMAA) differing in their MW and ratio of hydrophobic (BMA) and hydrophilic (MAA) units is prepared. Subsequently, using the biologically relevant model (T-cell line Jurkat), two compositions of BMAA copolymers are identified that solubilize cell membranes to an extent comparable to the industry standard, styrene-maleic acid copolymer (SMA), while avoiding the potentially problematic phenyl groups. Surprisingly, while only the lowest-MW variant of the BMA/MAA 2:1 composition is effective, all the copolymers of the BMA/MAA 1:1 composition are found to solubilize the model membranes, including the high-MW variant (MW of 14 000). Importantly, the density gradient ultracentrifugation/sodium dodecyl sulfate-polyacrylamide gel electrophoresis/Western blotting experiments reveal that the BMA/MAA 1:1 copolymers disintegrate the Jurkat membranes differently than SMA, as demonstrated by the different distribution patterns of two tested membrane protein markers. This makes the BMAA copolymers a useful tool for studies on membrane microdomains differing in their composition and resistance to membrane-disintegrating polymers.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

JANATA, Miroslav, Eva CADOVA, Pavla ANGELISOVA, Tatsiana CHARNAVETS, Vaclav HOREJSI a Vladimir RAUS. Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight. Macromolecular Bioscience. WEINHEIM: Wiley-VCH, 2022, roč. 22, č. 10, s. 2200284-2200290. ISSN 1616-5187. Dostupné z: https://dx.doi.org/10.1002/mabi.202200284.

@article{2262437,
   author = {Janata, Miroslav and Cadova, Eva and Angelisova, Pavla and Charnavets, Tatsiana and Horejsi, Vaclav and Raus, Vladimir},
   article_location = {WEINHEIM},
   article_number = {10},
   doi = {http://dx.doi.org/10.1002/mabi.202200284},
   keywords = {amphiphilic copolymers; isolation; membrane proteins; screening; solubilization},
   language = {eng},
   issn = {1616-5187},
   journal = {Macromolecular Bioscience},
   title = {Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200284},
   volume = {22},
   year = {2022}
}

TY  - JOUR
ID  - 2262437
AU  - Janata, Miroslav - Cadova, Eva - Angelisova, Pavla - Charnavets, Tatsiana - Horejsi, Vaclav - Raus, Vladimir
PY  - 2022
TI  - Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight
JF  - Macromolecular Bioscience
VL  - 22
IS  - 10
SP  - 2200284
EP  - 2200284
PB  - Wiley-VCH
SN  - 16165187
KW  - amphiphilic copolymers
KW  - isolation
KW  - membrane proteins
KW  - screening
KW  - solubilization
UR  - https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200284
N2  - Low-molecular weight (MW) amphiphilic copolymers have been recently introduced as a powerful tool for the detergent-free isolation of cell membrane proteins. Herein, a screening approach is used to identify a new copolymer type for this application. Via a two-step ATRP/acidolysis procedure, a 3 x 3 matrix of well-defined poly[(butyl methacrylate)-co-(methacrylic acid)] copolymers (denoted BMAA) differing in their MW and ratio of hydrophobic (BMA) and hydrophilic (MAA) units is prepared. Subsequently, using the biologically relevant model (T-cell line Jurkat), two compositions of BMAA copolymers are identified that solubilize cell membranes to an extent comparable to the industry standard, styrene-maleic acid copolymer (SMA), while avoiding the potentially problematic phenyl groups. Surprisingly, while only the lowest-MW variant of the BMA/MAA 2:1 composition is effective, all the copolymers of the BMA/MAA 1:1 composition are found to solubilize the model membranes, including the high-MW variant (MW of 14 000). Importantly, the density gradient ultracentrifugation/sodium dodecyl sulfate-polyacrylamide gel electrophoresis/Western blotting experiments reveal that the BMA/MAA 1:1 copolymers disintegrate the Jurkat membranes differently than SMA, as demonstrated by the different distribution patterns of two tested membrane protein markers. This makes the BMAA copolymers a useful tool for studies on membrane microdomains differing in their composition and resistance to membrane-disintegrating polymers.
ER  -

JANATA, Miroslav, Eva CADOVA, Pavla ANGELISOVA, Tatsiana CHARNAVETS, Vaclav HOREJSI a Vladimir RAUS. Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight. \textit{Macromolecular Bioscience}. WEINHEIM: Wiley-VCH, 2022, roč.~22, č.~10, s.~2200284-2200290. ISSN~1616-5187. Dostupné z: https://dx.doi.org/10.1002/mabi.202200284.

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