FOXO4 interacts with p53 TAD and CRD and inhibits its binding
to DNA

J 2022

FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA

MANDAL, Raju; Klara KOHOUTOVA; Olivia PETRVALSKA; Matej HORVATH; Pavel SRB et al.

Základní údaje

Originální název

FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA

Autoři

MANDAL, Raju; Klara KOHOUTOVA; Olivia PETRVALSKA; Matej HORVATH; Pavel SRB; Vaclav VEVERKA; Veronika OBSILOVA a Tomas OBSIL

Vydání

Protein Science, HOBOKEN, JOHN WILEY & SONS INC, 2022, 0961-8368

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:90127/22:00140005

Organizační jednotka

CIISB II

Klíčová slova anglicky

DNA binding; Forkhead box O 4; nuclear magnetic resonance; protein-protein interaction; senescence; transcription factor p53

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 5. 2025 11:28, Mgr. Eva Dubská

Anotace

V originále

Transcription factor p53 protects cells against tumorigenesis when subjected to various cellular stresses. Under these conditions, p53 interacts with transcription factor Forkhead box O (FOXO) 4, thereby inducing cellular senescence by upregulating the transcription of senescence-associated protein p21. However, the structural details of this interaction remain unclear. Here, we characterize the interaction between p53 and FOXO4 by NMR, chemical cross-linking, and analytical ultracentrifugation. Our results reveal that the interaction between p53 TAD and the FOXO4 Forkhead domain is essential for the overall stability of the p53:FOXO4 complex. Furthermore, contacts involving the N-terminal segment of FOXO4, the C-terminal negative regulatory domain of p53 and the DNA-binding domains of both proteins stabilize the complex, whose formation blocks p53 binding to DNA but without affecting the DNA-binding properties of FOXO4. Therefore, our structural findings may help to understand the intertwined functions of p53 and FOXO4 in cellular homeostasis, longevity, and stress response.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

MANDAL, Raju; Klara KOHOUTOVA; Olivia PETRVALSKA; Matej HORVATH; Pavel SRB; Vaclav VEVERKA; Veronika OBSILOVA a Tomas OBSIL. FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA. Protein Science. HOBOKEN: JOHN WILEY & SONS INC, 2022, roč. 31, č. 5, s. 1-13. ISSN 0961-8368. Dostupné z: https://doi.org/10.1002/pro.4287.

@article{2262447,
   author = {Mandal, Raju and Kohoutova, Klara and Petrvalska, Olivia and Horvath, Matej and Srb, Pavel and Veverka, Vaclav and Obsilova, Veronika and Obsil, Tomas},
   article_location = {HOBOKEN},
   article_number = {5},
   doi = {https://doi.org/10.1002/pro.4287},
   keywords = {DNA binding; Forkhead box O 4; nuclear magnetic resonance; protein-protein interaction; senescence; transcription factor p53},
   language = {eng},
   issn = {0961-8368},
   journal = {Protein Science},
   title = {FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/pro.4287},
   volume = {31},
   year = {2022}
}

TY  - JOUR
ID  - 2262447
AU  - Mandal, Raju - Kohoutova, Klara - Petrvalska, Olivia - Horvath, Matej - Srb, Pavel - Veverka, Vaclav - Obsilova, Veronika - Obsil, Tomas
PY  - 2022
TI  - FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA
JF  - Protein Science
VL  - 31
IS  - 5
SP  - 1-13
EP  - 1-13
PB  - JOHN WILEY & SONS INC
SN  - 09618368
KW  - DNA binding
KW  - Forkhead box O 4
KW  - nuclear magnetic resonance
KW  - protein-protein interaction
KW  - senescence
KW  - transcription factor p53
UR  - https://onlinelibrary.wiley.com/doi/10.1002/pro.4287
N2  - Transcription factor p53 protects cells against tumorigenesis when subjected to various cellular stresses. Under these conditions, p53 interacts with transcription factor Forkhead box O (FOXO) 4, thereby inducing cellular senescence by upregulating the transcription of senescence-associated protein p21. However, the structural details of this interaction remain unclear. Here, we characterize the interaction between p53 and FOXO4 by NMR, chemical cross-linking, and analytical ultracentrifugation. Our results reveal that the interaction between p53 TAD and the FOXO4 Forkhead domain is essential for the overall stability of the p53:FOXO4 complex. Furthermore, contacts involving the N-terminal segment of FOXO4, the C-terminal negative regulatory domain of p53 and the DNA-binding domains of both proteins stabilize the complex, whose formation blocks p53 binding to DNA but without affecting the DNA-binding properties of FOXO4. Therefore, our structural findings may help to understand the intertwined functions of p53 and FOXO4 in cellular homeostasis, longevity, and stress response.
ER  -

MANDAL, Raju; Klara KOHOUTOVA; Olivia PETRVALSKA; Matej HORVATH; Pavel SRB; Vaclav VEVERKA; Veronika OBSILOVA a Tomas OBSIL. FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA. \textit{Protein Science}. HOBOKEN: JOHN WILEY \&{}amp; SONS INC, 2022, roč.~31, č.~5, s.~1-13. ISSN~0961-8368. Dostupné z: https://doi.org/10.1002/pro.4287.

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