Dual specificity phosphatase 7 drives the formation of cardiac
mesoderm in mouse embryonic stem cells

J 2022

Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells

SLADEČEK, Stanislava; Katarzyna Anna RADASZKIEWICZ; Martina BŐHMOVÁ; Tomáš GYBEĽ; Tomasz Witold RADASZKIEWICZ et al.

Základní údaje

Originální název

Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells

Autoři

SLADEČEK, Stanislava; Katarzyna Anna RADASZKIEWICZ; Martina BŐHMOVÁ; Tomáš GYBEĽ; Tomasz Witold RADASZKIEWICZ a Jiří PACHERNÍK

Vydání

Plos one, San Francisco, Public Library of Science, 2022, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00128793

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cell differentiation; Cardiomyocytes; Cell staining; Heart; Mesoderm; Small interfering RNA; Phosphorylation; MAPK signaling cascades

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 3. 2023 09:37, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Dual specificity phosphatase 7 (DUSP7) is a protein belonging to a broad group of phosphatases that can dephosphorylate phosphoserine/phosphothreonine as well as phosphotyrosine residues within the same substrate. DUSP7 has been linked to the negative regulation of mitogen activated protein kinases (MAPK), and in particular to the regulation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). MAPKs play an important role in embryonic development, where their duration, magnitude, and spatiotemporal activity must be strictly controlled by other proteins, among others by DUSPs. In this study, we focused on the effect of DUSP7 depletion on the in vitro differentiation of mouse embryonic stem (ES) cells. We showed that even though DUSP7 knock-out ES cells do retain some of their basic characteristics, when it comes to differentiation, they preferentially differentiate towards neural cells, while the formation of early cardiac mesoderm is repressed. Therefore, our data indicate that DUSP7 is necessary for the correct formation of neuroectoderm and cardiac mesoderm during the in vitro differentiation of ES cells.

Návaznosti

GA18-18235S, projekt VaV

Název: Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk (Akronym: KarPKB)

Investor: Grantová agentura ČR, Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk

GA19-16861S, projekt VaV

Název: Interakce biomateriálů s kmenovými buňkami v simulovaných in vivo podmínkách (Akronym: IBMSKB)

Investor: Grantová agentura ČR, Interakce biomateriálů s kmenovými buňkami v simulovaných in vivo podmínkách

MUNI/A/1145/2017, interní kód MU

Název: Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018 (Akronym: OFIŽ 2018)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

SLADEČEK, Stanislava; Katarzyna Anna RADASZKIEWICZ; Martina BŐHMOVÁ; Tomáš GYBEĽ; Tomasz Witold RADASZKIEWICZ a Jiří PACHERNÍK. Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells. Plos one. San Francisco: Public Library of Science, 2022, roč. 17, č. 10, s. 1-22. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0275860.

@article{2263177,
   author = {Sladeček, Stanislava and Radaszkiewicz, Katarzyna Anna and Bőhmová, Martina and Gybeľ, Tomáš and Radaszkiewicz, Tomasz Witold and Pacherník, Jiří},
   article_location = {San Francisco},
   article_number = {10},
   doi = {https://doi.org/10.1371/journal.pone.0275860},
   keywords = {Cell differentiation; Cardiomyocytes; Cell staining; Heart; Mesoderm; Small interfering RNA; Phosphorylation; MAPK signaling cascades},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells},
   url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0275860},
   volume = {17},
   year = {2022}
}

TY  - JOUR
ID  - 2263177
AU  - Sladeček, Stanislava - Radaszkiewicz, Katarzyna Anna - Bőhmová, Martina - Gybeľ, Tomáš - Radaszkiewicz, Tomasz Witold - Pacherník, Jiří
PY  - 2022
TI  - Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells
JF  - Plos one
VL  - 17
IS  - 10
SP  - 1-22
EP  - 1-22
PB  - Public Library of Science
SN  - 19326203
KW  - Cell differentiation
KW  - Cardiomyocytes
KW  - Cell staining
KW  - Heart
KW  - Mesoderm
KW  - Small interfering RNA
KW  - Phosphorylation
KW  - MAPK signaling cascades
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0275860
N2  - Dual specificity phosphatase 7 (DUSP7) is a protein belonging to a broad group of phosphatases that can dephosphorylate phosphoserine/phosphothreonine as well as phosphotyrosine residues within the same substrate. DUSP7 has been linked to the negative regulation of mitogen activated protein kinases (MAPK), and in particular to the regulation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). MAPKs play an important role in embryonic development, where their duration, magnitude, and spatiotemporal activity must be strictly controlled by other proteins, among others by DUSPs. In this study, we focused on the effect of DUSP7 depletion on the in vitro differentiation of mouse embryonic stem (ES) cells. We showed that even though DUSP7 knock-out ES cells do retain some of their basic characteristics, when it comes to differentiation, they preferentially differentiate towards neural cells, while the formation of early cardiac mesoderm is repressed. Therefore, our data indicate that DUSP7 is necessary for the correct formation of neuroectoderm and cardiac mesoderm during the in vitro differentiation of ES cells.
ER  -

SLADEČEK, Stanislava; Katarzyna Anna RADASZKIEWICZ; Martina B$\backslash$H OHMOVÁ; Tomáš GYBEĽ; Tomasz Witold RADASZKIEWICZ a Jiří PACHERNÍK. Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells. \textit{Plos one}. San Francisco: Public Library of Science, 2022, roč.~17, č.~10, s.~1-22. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0275860.

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