Substantial decrease in SARS-CoV-2 RNA after fixation of
cadavers intended for anatomical dissection

J 2023

Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection

VYMAZALOVÁ, Kateřina; Omar ŠERÝ; Petr KRÁLÍK; Radka DZIEDZINSKÁ; Zuzana MUSILOVÁ et. al.

Základní údaje

Originální název

Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection

Autoři

VYMAZALOVÁ, Kateřina; Omar ŠERÝ; Petr KRÁLÍK; Radka DZIEDZINSKÁ; Zuzana MUSILOVÁ; Jan FRIŠHONS; Tomáš VOJTÍŠEK a Marek JOUKAL

Vydání

Anatomical Science International, NEW YORK, SPRINGER, 2023, 1447-6959

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30106 Anatomy and morphology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.200

Kód RIV

RIV/00216224:14110/23:00130424

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1007/s12565-023-00707-9

UT WoS

000943330200001

EID Scopus

2-s2.0-85149340443

Klíčová slova anglicky

Embalming; Dissection; Infections; Coronavirus; Polymerase chain reaction

Štítky

14110111, 14110514, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 1. 2024 11:14, Mgr. Tereza Miškechová

Anotace

V originále

With the onset of the COVID-19 pandemic, a problem arose with classic body donation programmes for obtaining cadavers for anatomical dissections, science and research. The question has emerged whether bodies of individuals who died of COVID-19 or were infected by SARS-CoV-2 could be admitted to Departments of Anatomy. To determine the risk of SARS-CoV-2 transmission to employees or students, the presence and stability of SARS-CoV-2 RNA in cadavers after fixation agents’ application and subsequent post-fixation baths over time were examined. The presence of viral RNA in swabs from selected tissues was assessed by the standardized routine RNA isolation protocol and subsequent real-time PCR analysis. To support the results obtained from the tissue swabs, samples of RNA were exposed in vitro to short and long-term exposure to the components of the injection and fixation solutions used for the bodies’ conservation. Substantial removal of SARS-CoV-2 RNA was observed in post-mortem tissue following perfusion with 3.5% phenol, 2.2% formaldehyde, 11.8% glycerol and 55% ethanol, and subsequent post-fixation in an ethanol bath. In vitro experiments showed significant effects of formaldehyde on SARS-CoV-2 RNA, while phenol and ethanol showed only negligible effects. We conclude that cadavers subjected to fixation protocols as described here should not pose a considerable risk of SARS-CoV-2 infection while being handled by students and staff and are, therefore, suitable for routine anatomical dissections and teaching.

Citovat

VYMAZALOVÁ, Kateřina; Omar ŠERÝ; Petr KRÁLÍK; Radka DZIEDZINSKÁ; Zuzana MUSILOVÁ; Jan FRIŠHONS; Tomáš VOJTÍŠEK a Marek JOUKAL. Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection. Anatomical Science International. NEW YORK: SPRINGER, 2023, roč. 98, č. 3, s. 441-447. ISSN 1447-6959. Dostupné z: https://doi.org/10.1007/s12565-023-00707-9.

@article{2266098,
   author = {Vymazalová, Kateřina and Šerý, Omar and Králík, Petr and Dziedzinská, Radka and Musilová, Zuzana and Frišhons, Jan and Vojtíšek, Tomáš and Joukal, Marek},
   article_location = {NEW YORK},
   article_number = {3},
   doi = {https://doi.org/10.1007/s12565-023-00707-9},
   keywords = {Embalming; Dissection; Infections; Coronavirus; Polymerase chain reaction},
   language = {eng},
   issn = {1447-6959},
   journal = {Anatomical Science International},
   title = {Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection},
   url = {https://link.springer.com/article/10.1007/s12565-023-00707-9},
   volume = {98},
   year = {2023}
}

TY  - JOUR
ID  - 2266098
AU  - Vymazalová, Kateřina - Šerý, Omar - Králík, Petr - Dziedzinská, Radka - Musilová, Zuzana - Frišhons, Jan - Vojtíšek, Tomáš - Joukal, Marek
PY  - 2023
TI  - Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection
JF  - Anatomical Science International
VL  - 98
IS  - 3
SP  - 441-447
EP  - 441-447
PB  - SPRINGER
SN  - 14476959
KW  - Embalming
KW  - Dissection
KW  - Infections
KW  - Coronavirus
KW  - Polymerase chain reaction
UR  - https://link.springer.com/article/10.1007/s12565-023-00707-9
N2  - With the onset of the COVID-19 pandemic, a problem arose with classic body donation programmes for obtaining cadavers for anatomical dissections, science and research. The question has emerged whether bodies of individuals who died of COVID-19 or were infected by SARS-CoV-2 could be admitted to Departments of Anatomy. To determine the risk of SARS-CoV-2 transmission to employees or students, the presence and stability of SARS-CoV-2 RNA in cadavers after fixation agents’ application and subsequent post-fixation baths over time were examined. The presence of viral RNA in swabs from selected tissues was assessed by the standardized routine RNA isolation protocol and subsequent real-time PCR analysis. To support the results obtained from the tissue swabs, samples of RNA were exposed in vitro to short and long-term exposure to the components of the injection and fixation solutions used for the bodies’ conservation. Substantial removal of SARS-CoV-2 RNA was observed in post-mortem tissue following perfusion with 3.5% phenol, 2.2% formaldehyde, 11.8% glycerol and 55% ethanol, and subsequent post-fixation in an ethanol bath. In vitro experiments showed significant effects of formaldehyde on SARS-CoV-2 RNA, while phenol and ethanol showed only negligible effects. We conclude that cadavers subjected to fixation protocols as described here should not pose a considerable risk of SARS-CoV-2 infection while being handled by students and staff and are, therefore, suitable for routine anatomical dissections and teaching.
ER  -

VYMAZALOVÁ, Kateřina; Omar ŠERÝ; Petr KRÁLÍK; Radka DZIEDZINSKÁ; Zuzana MUSILOVÁ; Jan FRIŠHONS; Tomáš VOJTÍŠEK a Marek JOUKAL. Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection. \textit{Anatomical Science International}. NEW YORK: SPRINGER, 2023, roč.~98, č.~3, s.~441-447. ISSN~1447-6959. Dostupné z: https://doi.org/10.1007/s12565-023-00707-9.

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