| BIANCHINI, Filippo, Virginia CRIVELLI, Morgan E. ABERNATHY, Concetta GUERRA, Martin PALUS, Jonathan MURI, Harold MARCOTTE, Antonio PIRALLA, Mattia PEDOTTI, Raoul DE GASPARO, Luca SIMONELLI, Milos MATKOVIC, Chiara TOSCANO, Maira BIGGIOGERO, Veronica CALVARUSO, Pavel SVOBODA, Tomás CERVANTES RINCÓN, Tommaso FAVA, Lucie PODEŠVOVÁ, Akanksha A. SHANBHAG, Andrea CELORIA, Jacopo SGRIGNANI, Michal STEFANIK, Václav HÖNIG, Veronika PRANCLOVA, Tereza MICHALCIKOVA, Jan PROCHAZKA, Giuditta GUERRINI, Dora MEHN, Annalisa CIABATTINI, Hassan ABOLHASSANI, David JARROSSAY, Mariagrazia UGUCCIONI, Donata MEDAGLINI, Qiang PAN-HAMMARSTRÖM, Luigi CALZOLAI, Daniel FERNANDEZ, Fausto BALDANTI, Alessandra FRANZETTI-PELLANDA, Christian GARZONI, Radislav SEDLACEK, Daniel RŮŽEK, Luca VARANI, Andrea CAVALLI, Christopher O. BARNES a Davide F. ROBBIANI. Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein. Science Immunology. American Association for the Advancement of Science, 2023, roč. 8, č. 81, s. 1-17. ISSN 2470-9468. Dostupné z: https://dx.doi.org/10.1126/sciimmunol.ade0958. |
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@article{2266937,
author = {Bianchini, Filippo and Crivelli, Virginia and Abernathy, Morgan E. and Guerra, Concetta and Palus, Martin and Muri, Jonathan and Marcotte, Harold and Piralla, Antonio and Pedotti, Mattia and De Gasparo, Raoul and Simonelli, Luca and Matkovic, Milos and Toscano, Chiara and Biggiogero, Maira and Calvaruso, Veronica and Svoboda, Pavel and Cervantes Rincón, Tomás and Fava, Tommaso and Podešvová, Lucie and Shanbhag, Akanksha A. and Celoria, Andrea and Sgrignani, Jacopo and Stefanik, Michal and Hönig, Václav and Pranclova, Veronika and Michalcikova, Tereza and Prochazka, Jan and Guerrini, Giuditta and Mehn, Dora and Ciabattini, Annalisa and Abolhassani, Hassan and Jarrossay, David and Uguccioni, Mariagrazia and Medaglini, Donata and PanandHammarström, Qiang and Calzolai, Luigi and Fernandez, Daniel and Baldanti, Fausto and FranzettiandPellanda, Alessandra and Garzoni, Christian and Sedlacek, Radislav and Růžek, Daniel and Varani, Luca and Cavalli, Andrea and Barnes, Christopher O. and Robbiani, Davide F.},
article_number = {81},
doi = {http://dx.doi.org/10.1126/sciimmunol.ade0958},
keywords = {SARS-CoV-2; coronavirus; vaccines; antiviral monoclonal antibodies},
language = {eng},
issn = {2470-9468},
journal = {Science Immunology},
title = {Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein},
url = {https://doi.org/10.1126/sciimmunol.ade0958},
volume = {8},
year = {2023}
}
TY - JOUR
ID - 2266937
AU - Bianchini, Filippo - Crivelli, Virginia - Abernathy, Morgan E. - Guerra, Concetta - Palus, Martin - Muri, Jonathan - Marcotte, Harold - Piralla, Antonio - Pedotti, Mattia - De Gasparo, Raoul - Simonelli, Luca - Matkovic, Milos - Toscano, Chiara - Biggiogero, Maira - Calvaruso, Veronica - Svoboda, Pavel - Cervantes Rincón, Tomás - Fava, Tommaso - Podešvová, Lucie - Shanbhag, Akanksha A. - Celoria, Andrea - Sgrignani, Jacopo - Stefanik, Michal - Hönig, Václav - Pranclova, Veronika - Michalcikova, Tereza - Prochazka, Jan - Guerrini, Giuditta - Mehn, Dora - Ciabattini, Annalisa - Abolhassani, Hassan - Jarrossay, David - Uguccioni, Mariagrazia - Medaglini, Donata - Pan-Hammarström, Qiang - Calzolai, Luigi - Fernandez, Daniel - Baldanti, Fausto - Franzetti-Pellanda, Alessandra - Garzoni, Christian - Sedlacek, Radislav - Růžek, Daniel - Varani, Luca - Cavalli, Andrea - Barnes, Christopher O. - Robbiani, Davide F.
PY - 2023
TI - Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein
JF - Science Immunology
VL - 8
IS - 81
SP - 1-17
EP - 1-17
PB - American Association for the Advancement of Science
SN - 24709468
KW - SARS-CoV-2
KW - coronavirus
KW - vaccines
KW - antiviral monoclonal antibodies
UR - https://doi.org/10.1126/sciimmunol.ade0958
N2 - Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy of vaccines and antiviral monoclonal antibodies. Continued development of immunotherapies and vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions of the virus spike glycoprotein that are both functionally relevant and averse to change, we identified human neutralizing antibodies to highly conserved viral epitopes. Antibody fp.006 binds the fusion peptide and cross-reacts against coronaviruses of the four genera, including the nine human coronaviruses, through recognition of a conserved motif that includes the S2′ site of proteolytic cleavage. Antibody hr2.016 targets the stem helix and neutralizes SARS-CoV-2 variants. Antibody sd1.040 binds to subdomain 1, synergizes with antibody rbd.042 for neutralization, and, similar to fp.006 and hr2.016, protects mice expressing human angiotensin-converting enzyme 2 against infection when present as a bispecific antibody. Thus, coldspot-guided antibody discovery reveals donor-derived neutralizing antibodies that are cross-reactive with Orthocoronavirinae, including SARS-CoV-2 variants.
ER -
BIANCHINI, Filippo, Virginia CRIVELLI, Morgan E. ABERNATHY, Concetta GUERRA, Martin PALUS, Jonathan MURI, Harold MARCOTTE, Antonio PIRALLA, Mattia PEDOTTI, Raoul DE GASPARO, Luca SIMONELLI, Milos MATKOVIC, Chiara TOSCANO, Maira BIGGIOGERO, Veronica CALVARUSO, Pavel SVOBODA, Tomás CERVANTES RINCÓN, Tommaso FAVA, Lucie PODEŠVOVÁ, Akanksha A. SHANBHAG, Andrea CELORIA, Jacopo SGRIGNANI, Michal STEFANIK, Václav HÖNIG, Veronika PRANCLOVA, Tereza MICHALCIKOVA, Jan PROCHAZKA, Giuditta GUERRINI, Dora MEHN, Annalisa CIABATTINI, Hassan ABOLHASSANI, David JARROSSAY, Mariagrazia UGUCCIONI, Donata MEDAGLINI, Qiang PAN-HAMMARSTRÖM, Luigi CALZOLAI, Daniel FERNANDEZ, Fausto BALDANTI, Alessandra FRANZETTI-PELLANDA, Christian GARZONI, Radislav SEDLACEK, Daniel RŮŽEK, Luca VARANI, Andrea CAVALLI, Christopher O. BARNES a Davide F. ROBBIANI. Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein. \textit{Science Immunology}. American Association for the Advancement of Science, 2023, roč.~8, č.~81, s.~1-17. ISSN~2470-9468. Dostupné z: https://dx.doi.org/10.1126/sciimmunol.ade0958.
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