| BRANDMEIER, Julian, Natalia JURGA, Tomasz GRZYB, Antonín HLAVÁČEK, Radka OBOŘILOVÁ, Petr SKLÁDAL, Zdeněk FARKA a Hans-Heiner GORRIS. Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay. Analytical Chemistry. Washington, DC: American Chemical Society, 2023, roč. 95, č. 10, s. 4753-4759. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.2c05670. |
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@article{2267281,
author = {Brandmeier, Julian and Jurga, Natalia and Grzyb, Tomasz and Hlaváček, Antonín and Obořilová, Radka and Skládal, Petr and Farka, Zdeněk and Gorris, HansandHeiner},
article_location = {Washington, DC},
article_number = {10},
doi = {http://dx.doi.org/10.1021/acs.analchem.2c05670},
keywords = {Covid-19; SARS-CoV-2; immunoassay; photon-upconversion nanoparticle; ULISA; digital detection},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay},
url = {https://pubs.acs.org/doi/10.1021/acs.analchem.2c05670},
volume = {95},
year = {2023}
}
TY - JOUR
ID - 2267281
AU - Brandmeier, Julian - Jurga, Natalia - Grzyb, Tomasz - Hlaváček, Antonín - Obořilová, Radka - Skládal, Petr - Farka, Zdeněk - Gorris, Hans-Heiner
PY - 2023
TI - Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay
JF - Analytical Chemistry
VL - 95
IS - 10
SP - 4753-4759
EP - 4753-4759
PB - American Chemical Society
SN - 00032700
KW - Covid-19
KW - SARS-CoV-2
KW - immunoassay
KW - photon-upconversion nanoparticle
KW - ULISA
KW - digital detection
UR - https://pubs.acs.org/doi/10.1021/acs.analchem.2c05670
N2 - The COVID-19 crisis requires fast and highly sensitive tests for the early stage detection of the SARS-CoV-2 virus. For detecting the nucleocapsid protein (N protein), the most abundant viral antigen, we have employed upconversion nanoparticles that emit short-wavelength light under near-infrared excitation (976 nm). The anti-Stokes emission avoids autofluorescence and light scattering and thus enables measurements without optical background interference. The sandwich upconversion-linked immunosorbent assay (ULISA) can be operated both in a conventional analog mode and in a digital mode based on counting individual immune complexes. We have investigated how different antibody combinations affect the detection of the wildtype N protein and the detection of SARS-CoV-2 (alpha variant) in lysed culture fluid via the N protein. The ULISA yielded a limit of detection (LOD) of 1.3 pg/mL (27 fM) for N protein detection independent of the analog or digital readout, which is approximately 3 orders of magnitude more sensitive than conventional enzyme-linked immunosorbent assays or commercial lateral flow assays for home testing. In the case of SARS-CoV-2, the digital ULISA additionally improved the LOD by a factor of 10 compared to the analog readout.
ER -
BRANDMEIER, Julian, Natalia JURGA, Tomasz GRZYB, Antonín HLAVÁČEK, Radka OBOŘILOVÁ, Petr SKLÁDAL, Zdeněk FARKA a Hans-Heiner GORRIS. Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, 2023, roč.~95, č.~10, s.~4753-4759. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.2c05670.
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