MORAVKOVA, Monika, Iveta KOSTOVOVA, Kateřina KAVANOVÁ, Radko PECHAR, Stanislav STANEK, Ales BRYCHTA, Michal ZEMAN a Tereza KUBASOVA. Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs. Microorganisms. Basel: MDPI, 2023, roč. 11, č. 1, s. 1-17. ISSN 2076-2607. Dostupné z: https://dx.doi.org/10.3390/microorganisms11010103. |
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@article{2267478, author = {Moravkova, Monika and Kostovova, Iveta and Kavanová, Kateřina and Pechar, Radko and Stanek, Stanislav and Brychta, Ales and Zeman, Michal and Kubasova, Tereza}, article_location = {Basel}, article_number = {1}, doi = {http://dx.doi.org/10.3390/microorganisms11010103}, keywords = {antibiotic resistance; tetW; ermB; Lactobacillus amylovorus; domestic pigs; wild boars}, language = {eng}, issn = {2076-2607}, journal = {Microorganisms}, title = {Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs}, url = {https://www.mdpi.com/2076-2607/11/1/103}, volume = {11}, year = {2023} }
TY - JOUR ID - 2267478 AU - Moravkova, Monika - Kostovova, Iveta - Kavanová, Kateřina - Pechar, Radko - Stanek, Stanislav - Brychta, Ales - Zeman, Michal - Kubasova, Tereza PY - 2023 TI - Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs JF - Microorganisms VL - 11 IS - 1 SP - 1-17 EP - 1-17 PB - MDPI SN - 20762607 KW - antibiotic resistance KW - tetW KW - ermB KW - Lactobacillus amylovorus KW - domestic pigs KW - wild boars UR - https://www.mdpi.com/2076-2607/11/1/103 N2 - Restrictions on the use of antibiotics in pigs lead to the continuous search for new probiotics serving as an alternative to antibiotics. One of the key parameters for probiotic bacteria selection is the absence of horizontally transmissible resistance genes. The aim of our study was to determine antibiotic susceptibility profiles in 28 Lactobacillus amylovorus isolates derived from the digestive tract of wild boars and farm pigs by means of the broth microdilution method and whole genome sequencing (WGS). We revealed genetic resistance determinants and examined sequences flanking resistance genes in these strains. Our findings indicate that L. amylovorus strains from domestic pigs are predominantly resistant to tetracycline, erythromycin and ampicillin. WGS analysis of horizontally transmissible genes revealed only three genetic determinants (tetW, ermB and aadE) of which all tetW and ermB genes were present only in strains derived from domestic pigs. Sequence analysis of coding sequences (CDS) in the neighborhood of the tetW gene revealed the presence of site-specific recombinase (xerC/D), site-specific DNA recombinase (spoIVCA) or DNA-binding transcriptional regulator (xre), usually directly downstream of the tetW gene. In the case of ermB, CDS for omega transcriptional repressor or mobilization protein were detected upstream of the ermB gene. ER -
MORAVKOVA, Monika, Iveta KOSTOVOVA, Kateřina KAVANOVÁ, Radko PECHAR, Stanislav STANEK, Ales BRYCHTA, Michal ZEMAN a Tereza KUBASOVA. Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs. \textit{Microorganisms}. Basel: MDPI, 2023, roč.~11, č.~1, s.~1-17. ISSN~2076-2607. Dostupné z: https://dx.doi.org/10.3390/microorganisms11010103.
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