What does critical temperature tell us about the resistence of
polar lichens to freezing stress? Applicability of linear
cooling method to ecophysiological studies

J 2022

What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies

HÁJEK, Josef, Anton PUHOVKIN, Davide GIORDANO a Jiří SEKERÁK

Základní údaje

Originální název

What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies

Autoři

HÁJEK, Josef (203 Česká republika, garant, domácí), Anton PUHOVKIN (804 Ukrajina, domácí), Davide GIORDANO (380 Itálie, domácí) a Jiří SEKERÁK (203 Česká republika, domácí)

Vydání

Czech Polar Reports, Brno, Masaryk University, 2022, 1805-0689

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.000

Kód RIV

RIV/00216224:14310/22:00128890

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.5817/CPR2022-2-18

UT WoS

000935360100008

Klíčová slova anglicky

cryoresistance; chlorophyll fluorescence; photosystem II; primary photosynthesis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 7. 2023 10:16, Mgr. Marie Šípková, DiS.

Anotace

V originále

Lichens from polar regions are well adapted to low temperature and considered cryoresistant. However, interspecific differences in their cryoresistance exist according to the degree of their adaptation and severity of the environment. In our study, we applied linear cooling technique in order to evaluate the interspecific differences in several lichen species. Thalli segments of Umbilicaria antarctica, Nephroma antarctica, Placopsis contortuplicata and Lasallia pustulata were exposed to the cooling from 20 to –35°C at a constant rate of 2°C min-1. Simultaneously with the cooling, chlorophyll fluorescence parameters evaluating potential (FV/FM) and effective yield of primary photochemical processes in PSII (FPSII) were measured in 30 s interval. Temperature response curves of FV/FM and FPSII formed typical S-curves that were species specific. Critical temperature (cooling point at which FPSII equals 0), was found in a narrow range of –25 to –28°C, suggesting that all experimental lichen species have a high resistance to sub-zero temperatures. The method of linear cooling used in this study has proven its applicability in ecophysiological studies since it is sensitive enough for the evaluation of species-specific differences in cryoresistance. This study describes different parameters that can be derived from the S-curves and discuss their proper use in ecophysiological and stress physiology studies.

Návaznosti

EF16_013/0001708, projekt VaV

Název: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)

Citovat

HÁJEK, Josef, Anton PUHOVKIN, Davide GIORDANO a Jiří SEKERÁK. What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies. Czech Polar Reports. Brno: Masaryk University, 2022, roč. 12, č. 2, s. 246-255. ISSN 1805-0689. Dostupné z: https://dx.doi.org/10.5817/CPR2022-2-18.

@article{2267728,
   author = {Hájek, Josef and Puhovkin, Anton and Giordano, Davide and Sekerák, Jiří},
   article_location = {Brno},
   article_number = {2},
   doi = {http://dx.doi.org/10.5817/CPR2022-2-18},
   keywords = {cryoresistance; chlorophyll fluorescence; photosystem II; primary photosynthesis},
   language = {eng},
   issn = {1805-0689},
   journal = {Czech Polar Reports},
   title = {What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies},
   url = {https://doi.org/10.5817/CPR2022-2-18},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 2267728
AU  - Hájek, Josef - Puhovkin, Anton - Giordano, Davide - Sekerák, Jiří
PY  - 2022
TI  - What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies
JF  - Czech Polar Reports
VL  - 12
IS  - 2
SP  - 246-255
EP  - 246-255
PB  - Masaryk University
SN  - 18050689
KW  - cryoresistance
KW  - chlorophyll fluorescence
KW  - photosystem II
KW  - primary photosynthesis
UR  - https://doi.org/10.5817/CPR2022-2-18
N2  - Lichens from polar regions are well adapted to low temperature and considered cryoresistant. However, interspecific differences in their cryoresistance exist according to the degree of their adaptation and severity of the environment. In our study, we applied linear cooling technique in order to evaluate the interspecific differences in several lichen species. Thalli segments of Umbilicaria antarctica, Nephroma antarctica, Placopsis contortuplicata and Lasallia pustulata were exposed to the cooling from 20 to –35°C at a constant rate of 2°C min-1. Simultaneously with the cooling, chlorophyll fluorescence parameters evaluating potential (FV/FM) and effective yield of primary photochemical processes in PSII (FPSII) were measured in 30 s interval. Temperature response curves of FV/FM and FPSII formed typical S-curves that were species specific. Critical temperature (cooling point at which FPSII equals 0), was found in a narrow range of –25 to –28°C, suggesting that all experimental lichen species have a high resistance to sub-zero temperatures. The method of linear cooling used in this study has proven its applicability in ecophysiological studies since it is sensitive enough for the evaluation of species-specific differences in cryoresistance. This study describes different parameters that can be derived from the S-curves and discuss their proper use in ecophysiological and stress physiology studies.
ER  -

HÁJEK, Josef, Anton PUHOVKIN, Davide GIORDANO a Jiří SEKERÁK. What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies. \textit{Czech Polar Reports}. Brno: Masaryk University, 2022, roč.~12, č.~2, s.~246-255. ISSN~1805-0689. Dostupné z: https://dx.doi.org/10.5817/CPR2022-2-18.

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