High-Throughput, Parallel Flow Cytometry Screening of Hundreds
of Cell Surface Antigens Using Fluorescent Barcoding

C 2022

High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding

DRÁPELA, Stanislav; Radek FEDR; Ondřej VACEK; Ján REMŠÍK; Karel SOUČEK et al.

Základní údaje

Originální název

High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding

Autoři

DRÁPELA, Stanislav; Radek FEDR; Ondřej VACEK; Ján REMŠÍK a Karel SOUČEK

Vydání

New York, Apoptosis and Cancer : Methods and Protocols, od s. 99-111, 13 s. Methods in Molecular Biology, 2022

Nakladatel

Humana Press Inc.

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

30204 Oncology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00128919

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-0716-2552-1

DOI

https://doi.org/10.1007/978-1-0716-2553-8_9

EID Scopus

2-s2.0-85138128132

Klíčová slova anglicky

Multicolor flow cytometry; Fluorescent cell barcoding; Cell surface phenotyping; High-throughput screening

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 3. 2023 11:16, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.

Návaznosti

EF16_025/0007381, projekt VaV

Název: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou

Citovat

DRÁPELA, Stanislav; Radek FEDR; Ondřej VACEK; Ján REMŠÍK a Karel SOUČEK. High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding. In Hugo Barcenilla; David Diaz. Apoptosis and Cancer : Methods and Protocols. New York: Humana Press Inc., 2022, s. 99-111. Methods in Molecular Biology. ISBN 978-1-0716-2552-1. Dostupné z: https://doi.org/10.1007/978-1-0716-2553-8_9.

@inbook{2269118,
   author = {Drápela, Stanislav and Fedr, Radek and Vacek, Ondřej and Remšík, Ján and Souček, Karel},
   address = {New York},
   booktitle = {Apoptosis and Cancer : Methods and Protocols},
   doi = {https://doi.org/10.1007/978-1-0716-2553-8_9},
   editor = {Hugo Barcenilla; David Diaz},
   keywords = {Multicolor flow cytometry; Fluorescent cell barcoding; Cell surface phenotyping; High-throughput screening},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-0716-2552-1},
   pages = {99-111},
   publisher = {Humana Press Inc.},
   title = {High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding},
   url = {https://doi.org/10.1007/978-1-0716-2553-8_9},
   year = {2022}
}

TY  - CHAP
ID  - 2269118
AU  - Drápela, Stanislav - Fedr, Radek - Vacek, Ondřej - Remšík, Ján - Souček, Karel
PY  - 2022
TI  - High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding
VL  - Methods in Molecular Biology
PB  - Humana Press Inc.
CY  - New York
SN  - 9781071625521
KW  - Multicolor flow cytometry
KW  - Fluorescent cell barcoding
KW  - Cell surface phenotyping
KW  - High-throughput screening
UR  - https://doi.org/10.1007/978-1-0716-2553-8_9
N2  - Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.
ER  -

DRÁPELA, Stanislav; Radek FEDR; Ondřej VACEK; Ján REMŠÍK a Karel SOUČEK. High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding. In Hugo Barcenilla; David Diaz. \textit{Apoptosis and Cancer : Methods and Protocols}. New York: Humana Press Inc., 2022, s.~99-111. Methods in Molecular Biology. ISBN~978-1-0716-2552-1. Dostupné z: https://doi.org/10.1007/978-1-0716-2553-8\_{}9.

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