The SAGA histone acetyltransferase module targets SMC5/6 to
specific genes

J 2023

The SAGA histone acetyltransferase module targets SMC5/6 to specific genes

MAHRÍK, Lenka; Barbora ŠTEFANOVIE; Anna MARESOVA; Jarmila PRINCOVA; Peter KOLESÁR et. al.

Základní údaje

Originální název

The SAGA histone acetyltransferase module targets SMC5/6 to specific genes

Autoři

MAHRÍK, Lenka; Barbora ŠTEFANOVIE; Anna MARESOVA; Jarmila PRINCOVA; Peter KOLESÁR; Edit LELKES; Celline FAUX; Dominique HELMLINGER; Martin PREVOROVSKY a Jan PALEČEK

Vydání

EPIGENETICS & CHROMATIN, LONDON, BMC, 2023, 1756-8935

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.200

Kód RIV

RIV/00216224:14310/23:00130526

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1186/s13072-023-00480-z

UT WoS

000944569400001

EID Scopus

2-s2.0-85148114883

Klíčová slova anglicky

Genetic and protein-protein interactions; SMC5/6 complex; Nse3 KITE; SAGA histone acetyltransferase module; Gcn5; Ada2; Chromatin accessibility; DNA repair; rDNA; Gene regions

Štítky

143160, CF GEN, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2024 09:23, Mgr. Eva Dubská

Anotace

V originále

Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC complexes (cohesin, condensin and SMC5/6) play key roles in cohesion, condensation, replication, transcription and DNA repair. Their physical binding to DNA requires accessible chromatin. We performed a genetic screen in fission yeast to identify novel factors required for SMC5/6 binding to DNA. We identified 79 genes of which histone acetyltransferases (HATs) were the most represented. Genetic and phenotypic analyses suggested a particularly strong functional relationship between the SMC5/6 and SAGA complexes. Furthermore, several SMC5/6 subunits physically interacted with SAGA HAT module components Gcn5 and Ada2. As Gcn5-dependent acetylation facilitates the accessibility of chromatin to DNA-repair proteins, we first analysed the formation of DNA-damage-induced SMC5/6 foci in the Delta gcn5 mutant. The SMC5/6 foci formed normally in Delta gcn5, suggesting SAGA-independent SMC5/6 localization to DNA-damaged sites. Next, we used Nse4-FLAG chromatin-immunoprecipitation (ChIP-seq) analysis in unchallenged cells to assess SMC5/6 distribution. A significant portion of SMC5/6 accumulated within gene regions in wild-type cells, which was reduced in Delta gcn5 and Delta ada2 mutants. The drop in SMC5/6 levels was also observed in gcn5-E191Q acetyltransferase-dead mutant. Our data show genetic and physical interactions between SMC5/6 and SAGA complexes. The ChIP-seq analysis suggests that SAGA HAT module targets SMC5/6 to specific gene regions and facilitates their accessibility for SMC5/6 loading.

Návaznosti

LM2018132, projekt VaV

Název: Národní centrum lékařské genomiky (Akronym: NCLG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní centrum lékařské genomiky

LTC20033, projekt VaV

Název: The SMC5/6 complex in nucleome organization (Akronym: Nukleom)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, The SMC5/6 complex in nucleome organization, INTER-COST

MUNI/R/1142/2021, interní kód MU

Název: Visualization of the 3D structure of chromatin

Investor: Masarykova univerzita, Visualization of the 3D structure of chromatin, CAREER RESTART

MUNI/R/1262/2022, interní kód MU

Název: Vztah mezi opravou DNA a transkripcí

Investor: Masarykova univerzita, Vztah mezi opravou DNA a transkripcí, CAREER RESTART

Citovat

MAHRÍK, Lenka; Barbora ŠTEFANOVIE; Anna MARESOVA; Jarmila PRINCOVA; Peter KOLESÁR; Edit LELKES; Celline FAUX; Dominique HELMLINGER; Martin PREVOROVSKY a Jan PALEČEK. The SAGA histone acetyltransferase module targets SMC5/6 to specific genes. EPIGENETICS & CHROMATIN. LONDON: BMC, 2023, roč. 16, č. 1, s. 1-16. ISSN 1756-8935. Dostupné z: https://doi.org/10.1186/s13072-023-00480-z.

@article{2271653,
   author = {Mahrík, Lenka and Štefanovie, Barbora and Maresova, Anna and Princova, Jarmila and Kolesár, Peter and Lelkes, Edit and Faux, Celline and Helmlinger, Dominique and Prevorovsky, Martin and Paleček, Jan},
   article_location = {LONDON},
   article_number = {1},
   doi = {https://doi.org/10.1186/s13072-023-00480-z},
   keywords = {Genetic and protein-protein interactions; SMC5/6 complex; Nse3 KITE; SAGA histone acetyltransferase module; Gcn5; Ada2; Chromatin accessibility; DNA repair; rDNA; Gene regions},
   language = {eng},
   issn = {1756-8935},
   journal = {EPIGENETICS & CHROMATIN},
   title = {The SAGA histone acetyltransferase module targets SMC5/6 to specific genes},
   url = {https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-023-00480-z},
   volume = {16},
   year = {2023}
}

TY  - JOUR
ID  - 2271653
AU  - Mahrík, Lenka - Štefanovie, Barbora - Maresova, Anna - Princova, Jarmila - Kolesár, Peter - Lelkes, Edit - Faux, Celline - Helmlinger, Dominique - Prevorovsky, Martin - Paleček, Jan
PY  - 2023
TI  - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes
JF  - EPIGENETICS & CHROMATIN
VL  - 16
IS  - 1
SP  - 1-16
EP  - 1-16
PB  - BMC
SN  - 17568935
KW  - Genetic and protein-protein interactions
KW  - SMC5/6 complex
KW  - Nse3 KITE
KW  - SAGA histone acetyltransferase module
KW  - Gcn5
KW  - Ada2
KW  - Chromatin accessibility
KW  - DNA repair
KW  - rDNA
KW  - Gene regions
UR  - https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-023-00480-z
N2  - Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC complexes (cohesin, condensin and SMC5/6) play key roles in cohesion, condensation, replication, transcription and DNA repair. Their physical binding to DNA requires accessible chromatin. We performed a genetic screen in fission yeast to identify novel factors required for SMC5/6 binding to DNA. We identified 79 genes of which histone acetyltransferases (HATs) were the most represented. Genetic and phenotypic analyses suggested a particularly strong functional relationship between the SMC5/6 and SAGA complexes. Furthermore, several SMC5/6 subunits physically interacted with SAGA HAT module components Gcn5 and Ada2. As Gcn5-dependent acetylation facilitates the accessibility of chromatin to DNA-repair proteins, we first analysed the formation of DNA-damage-induced SMC5/6 foci in the Delta gcn5 mutant. The SMC5/6 foci formed normally in Delta gcn5, suggesting SAGA-independent SMC5/6 localization to DNA-damaged sites. Next, we used Nse4-FLAG chromatin-immunoprecipitation (ChIP-seq) analysis in unchallenged cells to assess SMC5/6 distribution. A significant portion of SMC5/6 accumulated within gene regions in wild-type cells, which was reduced in Delta gcn5 and Delta ada2 mutants. The drop in SMC5/6 levels was also observed in gcn5-E191Q acetyltransferase-dead mutant. Our data show genetic and physical interactions between SMC5/6 and SAGA complexes. The ChIP-seq analysis suggests that SAGA HAT module targets SMC5/6 to specific gene regions and facilitates their accessibility for SMC5/6 loading.
ER  -

MAHRÍK, Lenka; Barbora ŠTEFANOVIE; Anna MARESOVA; Jarmila PRINCOVA; Peter KOLESÁR; Edit LELKES; Celline FAUX; Dominique HELMLINGER; Martin PREVOROVSKY a Jan PALEČEK. The SAGA histone acetyltransferase module targets SMC5/6 to specific genes. \textit{EPIGENETICS \&{} CHROMATIN}. LONDON: BMC, 2023, roč.~16, č.~1, s.~1-16. ISSN~1756-8935. Dostupné z: https://doi.org/10.1186/s13072-023-00480-z.

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