Effects of pesticide exposure on oxidative stress and DNA
methylation urinary biomarkers in Czech adults and children
from the CELSPAC-SPECIMEn cohort

J 2023

Effects of pesticide exposure on oxidative stress and DNA methylation urinary biomarkers in Czech adults and children from the CELSPAC-SPECIMEn cohort

JANOŠ, Tomáš; Ilse OTTENBROS; Lucie BLÁHOVÁ; Petr ŠENK; Libor ŠULC et al.

Základní údaje

Originální název

Effects of pesticide exposure on oxidative stress and DNA methylation urinary biomarkers in Czech adults and children from the CELSPAC-SPECIMEn cohort

Autoři

JANOŠ, Tomáš; Ilse OTTENBROS; Lucie BLÁHOVÁ; Petr ŠENK; Libor ŠULC; Nina PÁLEŠOVÁ; Jessica SHEARDOVÁ; Jelle VLAANDEREN a Pavel ČUPR

Vydání

Environmental Research, SAN DIEGO, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2023, 0013-9351

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00130567

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Epigenetics; DNA methylation; Oxidative stress; Current-use pesticides (CUPs); Urine

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 3. 2024 22:15, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Current-use pesticide (CUP) exposure occurs mainly through diet and environmental application in both agricultural and urban settings. While pesticide exposure has been associated with many adverse health outcomes, the intermediary molecular mechanisms are still not completely elucidated. Among others, their roles in epigenetics (DNA methylation) and DNA damage due to oxidative stress are presumed. Scientific evidence on urinary biomarkers of such body response in general population is limited, especially in children. A total of 440 urine samples (n = 110 parent-child pairs) were collected during the winter and summer seasons in order to describe levels of overall DNA methylation (5-mC, 5-mdC, 5-hmdC, 7-mG, 3-mA) and oxidative stress (8-OHdG) biomarkers and investigate their possible associations with metabolites of pyrethroids (3-PBA, t/cDCCA), chlorpyrifos (TCPY), and tebuconazole (TEB-OH). Linear mixed-effects models accounting for intraindividual and intrahousehold correlations were utilized. We applied false discovery rate procedure to account for multiplicity and adjusted for potential confounding variables. Higher urinary levels of most biological response biomarkers were measured in winter samples. In adjusted repeated measures models, interquartile range (IQR) increases in pyrethroid metabolites were associated with higher oxidative stress. t/c-DCCA and TCPY were associated with higher urinary levels of cytosine methylation biomarkers (5-mC and/or 5-mdC). The most robust association was observed for tebuconazole metabolite with 3mA (-15.1% change per IQR increase, 95% CI = -23.6, -5.69) suggesting a role of this pesticide in reduced demethylation processes through possible DNA glycosylase inhibition. Our results indicate an urgent need to extend the range of analyzed environmental chemicals such as azole pesticides (e.g. prothioconazole) in human biomonitoring studies. This is the first study to report urinary DNA methylation biomarkers in children and associations between CUP metabolites and a comprehensive set of biomarkers including methylated and oxidized DNA alterations. Observed associations warrant further largescale research of these biomarkers and environmental pollutants including CUPs.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

733032, interní kód MU

Název: European Human Biomonitoring Initiative (Akronym: HBM4EU)

Investor: Evropská unie, European Human Biomonitoring Initiative, Health, demographic change and wellbeing (Societal Challenges)

857340, interní kód MU

Název: External and Internal Human Exposure in Urban EXposome (Akronym: Urban_X)

Investor: Evropská unie, External and Internal Human Exposure in Urban EXposome, Spreading excellence and widening participation

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

874627, interní kód MU

Název: EXposome Powered tools for healthy living in urbAN SEttings (Akronym: EXPANSE)

Investor: Evropská unie, EXposome Powered tools for healthy living in urbAN SEttings, Health, demographic change and wellbeing (Societal Challenges)

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Přiložené soubory

2274378.pdf

Citovat

JANOŠ, Tomáš; Ilse OTTENBROS; Lucie BLÁHOVÁ; Petr ŠENK; Libor ŠULC; Nina PÁLEŠOVÁ; Jessica SHEARDOVÁ; Jelle VLAANDEREN a Pavel ČUPR. Effects of pesticide exposure on oxidative stress and DNA methylation urinary biomarkers in Czech adults and children from the CELSPAC-SPECIMEn cohort. Environmental Research. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2023, roč. 222, April 2023, s. 1-9. ISSN 0013-9351. Dostupné z: https://doi.org/10.1016/j.envres.2023.115368.

@article{2274378,
   author = {Janoš, Tomáš and Ottenbros, Ilse and Bláhová, Lucie and Šenk, Petr and Šulc, Libor and Pálešová, Nina and Sheardová, Jessica and Vlaanderen, Jelle and Čupr, Pavel},
   article_location = {SAN DIEGO},
   article_number = {April 2023},
   doi = {https://doi.org/10.1016/j.envres.2023.115368},
   keywords = {Epigenetics; DNA methylation; Oxidative stress; Current-use pesticides (CUPs); Urine},
   language = {eng},
   issn = {0013-9351},
   journal = {Environmental Research},
   title = {Effects of pesticide exposure on oxidative stress and DNA methylation urinary biomarkers in Czech adults and children from the CELSPAC-SPECIMEn cohort},
   url = {https://www.sciencedirect.com/science/article/pii/S0013935123001603?via%3Dihub},
   volume = {222},
   year = {2023}
}

TY  - JOUR
ID  - 2274378
AU  - Janoš, Tomáš - Ottenbros, Ilse - Bláhová, Lucie - Šenk, Petr - Šulc, Libor - Pálešová, Nina - Sheardová, Jessica - Vlaanderen, Jelle - Čupr, Pavel
PY  - 2023
TI  - Effects of pesticide exposure on oxidative stress and DNA methylation urinary biomarkers in Czech adults and children from the CELSPAC-SPECIMEn cohort
JF  - Environmental Research
VL  - 222
IS  - April 2023
SP  - 1-9
EP  - 1-9
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 00139351
KW  - Epigenetics
KW  - DNA methylation
KW  - Oxidative stress
KW  - Current-use pesticides (CUPs)
KW  - Urine
UR  - https://www.sciencedirect.com/science/article/pii/S0013935123001603?via%3Dihub
N2  - Current-use pesticide (CUP) exposure occurs mainly through diet and environmental application in both agricultural and urban settings. While pesticide exposure has been associated with many adverse health outcomes, the intermediary molecular mechanisms are still not completely elucidated. Among others, their roles in epigenetics (DNA methylation) and DNA damage due to oxidative stress are presumed. Scientific evidence on urinary biomarkers of such body response in general population is limited, especially in children. A total of 440 urine samples (n = 110 parent-child pairs) were collected during the winter and summer seasons in order to describe levels of overall DNA methylation (5-mC, 5-mdC, 5-hmdC, 7-mG, 3-mA) and oxidative stress (8-OHdG) biomarkers and investigate their possible associations with metabolites of pyrethroids (3-PBA, t/cDCCA), chlorpyrifos (TCPY), and tebuconazole (TEB-OH). Linear mixed-effects models accounting for intraindividual and intrahousehold correlations were utilized. We applied false discovery rate procedure to account for multiplicity and adjusted for potential confounding variables. Higher urinary levels of most biological response biomarkers were measured in winter samples. In adjusted repeated measures models, interquartile range (IQR) increases in pyrethroid metabolites were associated with higher oxidative stress. t/c-DCCA and TCPY were associated with higher urinary levels of cytosine methylation biomarkers (5-mC and/or 5-mdC). The most robust association was observed for tebuconazole metabolite with 3mA (-15.1% change per IQR increase, 95% CI = -23.6, -5.69) suggesting a role of this pesticide in reduced demethylation processes through possible DNA glycosylase inhibition. Our results indicate an urgent need to extend the range of analyzed environmental chemicals such as azole pesticides (e.g. prothioconazole) in human biomonitoring studies. This is the first study to report urinary DNA methylation biomarkers in children and associations between CUP metabolites and a comprehensive set of biomarkers including methylated and oxidized DNA alterations. Observed associations warrant further largescale research of these biomarkers and environmental pollutants including CUPs.
ER  -

JANOŠ, Tomáš; Ilse OTTENBROS; Lucie BLÁHOVÁ; Petr ŠENK; Libor ŠULC; Nina PÁLEŠOVÁ; Jessica SHEARDOVÁ; Jelle VLAANDEREN a Pavel ČUPR. Effects of pesticide exposure on oxidative stress and DNA methylation urinary biomarkers in Czech adults and children from the CELSPAC-SPECIMEn cohort. \textit{Environmental Research}. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2023, roč.~222, April 2023, s.~1-9. ISSN~0013-9351. Dostupné z: https://doi.org/10.1016/j.envres.2023.115368.

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