Microbiota, Phagocytic Activity, Biochemical Parameters and
Parasite Control in Horses with Application of Autochthonous,
Bacteriocin-Producing, Probiotic Strain Enterococcus faecium EF
412

J 2023

Microbiota, Phagocytic Activity, Biochemical Parameters and Parasite Control in Horses with Application of Autochthonous, Bacteriocin-Producing, Probiotic Strain Enterococcus faecium EF 412

LAUKOVÁ, Andrea; Lenka KOSEČKOVÁ MICENKOVÁ; Ivana KUBAŠOVÁ; Eva BINO; Anna KANDRIČÁKOVÁ et al.

Základní údaje

Originální název

Microbiota, Phagocytic Activity, Biochemical Parameters and Parasite Control in Horses with Application of Autochthonous, Bacteriocin-Producing, Probiotic Strain Enterococcus faecium EF 412

Autoři

Vydání

Probiotics and Antimicrobial Proteins, New York, Springer New York LLC, 2023, 1867-1306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.400

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00130676

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Horses; Health; Beneficial strain; Microbiota; Biochemical parameters; Parasites

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2024 08:16, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The beneficial influence of bacteriocin-producing, probiotic, mostly non-autochthonous bacteria has already been reported in various animals. However, their use in horses provides limited information, and results with autochthonous bacteria have not been reported. Therefore, the main objective of this model study was to test the effect of autochthonous, bacteriocin-producing faecal strain Enterococcus faecium EF 412 application in horses. One gram of freeze-dried EF 412 strain (10(9) CFU/mL for 21 days) was applied to horses in a small feed ball. Clinically healthy horses (12), Slovak warm-blood breed of various ages (5-13 years), were involved in a 35-day-long experiment, also functioning as control for themselves. They were stabled in separate boxes (university property), fed twice a day (hay, whole oats or grazed) with water access ad libitum. Sampling was performed at the start of the experiment, i.e. at days 0/1, 21 (3 weeks of EF 412 application) and at day 35 (2 weeks of EF 412 cessation). EF 412 colonized GIT of horses was 3.54 +/- 0.75 CFU/g (log 10) at day 21. The eggs of the nematode Strongylus spp. were not found in horses after EF 412 application, and Eimeria spp. oocysts were similarly not found. The other microbiota were not reduced as evaluated by the use of standard method. Using next-generation sequencing, at phylum level, phyla Bacteroidetes and Firmicutes dominated and at family level, they were Bacteroidales BS11 and S24-7 gut goups and Lentisphaerae. In horses, the increasing tendency in phagocytic activity was noted after EF 412 application. Biochemical parameters were in the physiological range. Total protein value was significantly decreased at day 21 compared with day 0/1 as well as with day 35 (P < 0.05). Cholesterol and triglycerides were influenced (decreased) at day 21 compared with day 0/1 and day 35. Neither nematode eggs Strongylus spp. nor Eimeria spp. oocysts were found in faeces after EF 412 application. Autochthonous, faecal strain E. faecium EF 412 showed promising application potential.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Citovat

LAUKOVÁ, Andrea; Lenka KOSEČKOVÁ MICENKOVÁ; Ivana KUBAŠOVÁ; Eva BINO; Anna KANDRIČÁKOVÁ; Iveta PLACHÁ; Gabriela ŠTRKOLCOVÁ; Branislav GÁLIK; Anton KOVÁČIK; Marko HALO a Monika Pogány SIMONOVÁ. Microbiota, Phagocytic Activity, Biochemical Parameters and Parasite Control in Horses with Application of Autochthonous, Bacteriocin-Producing, Probiotic Strain Enterococcus faecium EF 412. Probiotics and Antimicrobial Proteins. New York: Springer New York LLC, 2023, roč. 15, č. 1, s. 139-148. ISSN 1867-1306. Dostupné z: https://doi.org/10.1007/s12602-022-09918-4.

@article{2278452,
   author = {Lauková, Andrea and Kosečková Micenková, Lenka and Kubašová, Ivana and Bino, Eva and Kandričáková, Anna and Plachá, Iveta and Štrkolcová, Gabriela and Gálik, Branislav and Kováčik, Anton and Halo, Marko and Simonová, Monika Pogány},
   article_location = {New York},
   article_number = {1},
   doi = {https://doi.org/10.1007/s12602-022-09918-4},
   keywords = {Horses; Health; Beneficial strain; Microbiota; Biochemical parameters; Parasites},
   language = {eng},
   issn = {1867-1306},
   journal = {Probiotics and Antimicrobial Proteins},
   title = {Microbiota, Phagocytic Activity, Biochemical Parameters and Parasite Control in Horses with Application of Autochthonous, Bacteriocin-Producing, Probiotic Strain Enterococcus faecium EF 412},
   url = {https://link.springer.com/article/10.1007/s12602-022-09918-4},
   volume = {15},
   year = {2023}
}

TY  - JOUR
ID  - 2278452
AU  - Lauková, Andrea - Kosečková Micenková, Lenka - Kubašová, Ivana - Bino, Eva - Kandričáková, Anna - Plachá, Iveta - Štrkolcová, Gabriela - Gálik, Branislav - Kováčik, Anton - Halo, Marko - Simonová, Monika Pogány
PY  - 2023
TI  - Microbiota, Phagocytic Activity, Biochemical Parameters and Parasite Control in Horses with Application of Autochthonous, Bacteriocin-Producing, Probiotic Strain Enterococcus faecium EF 412
JF  - Probiotics and Antimicrobial Proteins
VL  - 15
IS  - 1
SP  - 139-148
EP  - 139-148
PB  - Springer New York LLC
SN  - 18671306
KW  - Horses
KW  - Health
KW  - Beneficial strain
KW  - Microbiota
KW  - Biochemical parameters
KW  - Parasites
UR  - https://link.springer.com/article/10.1007/s12602-022-09918-4
N2  - The beneficial influence of bacteriocin-producing, probiotic, mostly non-autochthonous bacteria has already been reported in various animals. However, their use in horses provides limited information, and results with autochthonous bacteria have not been reported. Therefore, the main objective of this model study was to test the effect of autochthonous, bacteriocin-producing faecal strain Enterococcus faecium EF 412 application in horses. One gram of freeze-dried EF 412 strain (10(9) CFU/mL for 21 days) was applied to horses in a small feed ball. Clinically healthy horses (12), Slovak warm-blood breed of various ages (5-13 years), were involved in a 35-day-long experiment, also functioning as control for themselves. They were stabled in separate boxes (university property), fed twice a day (hay, whole oats or grazed) with water access ad libitum. Sampling was performed at the start of the experiment, i.e. at days 0/1, 21 (3 weeks of EF 412 application) and at day 35 (2 weeks of EF 412 cessation). EF 412 colonized GIT of horses was 3.54 +/- 0.75 CFU/g (log 10) at day 21. The eggs of the nematode Strongylus spp. were not found in horses after EF 412 application, and Eimeria spp. oocysts were similarly not found. The other microbiota were not reduced as evaluated by the use of standard method. Using next-generation sequencing, at phylum level, phyla Bacteroidetes and Firmicutes dominated and at family level, they were Bacteroidales BS11 and S24-7 gut goups and Lentisphaerae. In horses, the increasing tendency in phagocytic activity was noted after EF 412 application. Biochemical parameters were in the physiological range. Total protein value was significantly decreased at day 21 compared with day 0/1 as well as with day 35 (P < 0.05). Cholesterol and triglycerides were influenced (decreased) at day 21 compared with day 0/1 and day 35. Neither nematode eggs Strongylus spp. nor Eimeria spp. oocysts were found in faeces after EF 412 application. Autochthonous, faecal strain E. faecium EF 412 showed promising application potential.
ER  -

LAUKOVÁ, Andrea; Lenka KOSEČKOVÁ MICENKOVÁ; Ivana KUBAŠOVÁ; Eva BINO; Anna KANDRIČÁKOVÁ; Iveta PLACHÁ; Gabriela ŠTRKOLCOVÁ; Branislav GÁLIK; Anton KOVÁČIK; Marko HALO a Monika Pogány SIMONOVÁ. Microbiota, Phagocytic Activity, Biochemical Parameters and Parasite Control in Horses with Application of Autochthonous, Bacteriocin-Producing, Probiotic Strain Enterococcus faecium EF 412. \textit{Probiotics and Antimicrobial Proteins}. New York: Springer New York LLC, 2023, roč.~15, č.~1, s.~139-148. ISSN~1867-1306. Dostupné z: https://doi.org/10.1007/s12602-022-09918-4.

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