Insights into HPLC-MS/MS Analysis, Antioxidant and Cytotoxic
Activity of Astragalus fruticosus against Different Types of
Cancer Cell Lines

J 2022

Insights into HPLC-MS/MS Analysis, Antioxidant and Cytotoxic Activity of Astragalus fruticosus against Different Types of Cancer Cell Lines

DEKINASH, Mohamed Fayez, Tarek M OKDA, Ehab Kotb ELMAHALLAWY, Fathy Kandil EL-FIKY, Gamal Abd El Hay OMRAN et. al.

Základní údaje

Originální název

Insights into HPLC-MS/MS Analysis, Antioxidant and Cytotoxic Activity of Astragalus fruticosus against Different Types of Cancer Cell Lines

Autoři

DEKINASH, Mohamed Fayez, Tarek M OKDA (garant), Ehab Kotb ELMAHALLAWY, Fathy Kandil EL-FIKY, Gamal Abd El Hay OMRAN, Emil ŠVAJDLENKA (203 Česká republika, domácí), Naief DAHRAN, Manal F EL-KHADRAGY, Wafa A AL-MEGRIN a El Naggar El Moataz Bellah ALI

Vydání

Pharmaceuticals, Basel, MDPI, 2022, 1424-8247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600

Kód RIV

RIV/00216224:14160/22:00129770

Organizační jednotka

Farmaceutická fakulta

DOI

http://dx.doi.org/10.3390/ph15111406

UT WoS

000895690000001

Klíčová slova anglicky

Astragalus fruticosus; HPLC-MS; MS profiling; colorectal cancer; cytotoxic activity

Štítky

rivok, ÚPL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 5. 2023 21:38, JUDr. Sabina Krejčiříková

Anotace

V originále

Plants from the genus Astragalus are gaining attention for their pharmacological importance. However, the information available regarding the HPLC–MS/MS chemical profile of A. fruticosus is inadequate. In this study, we performed HPLC–MS/MS analysis using electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). We tentatively identified 11 compounds in the A. fruticosus methanolic extract, including five flavonoidal and six saponin glycosides. The extract showed moderate antioxidant activity with 21.05% reduction in DPPH UV absorption. The preliminary cytotoxic screening against seven human cancer cell lines using 100 μg/mL extract showed prominent cytotoxic potential against colorectal cancer HCT–116 with 3.368% cell viability. It also showed moderate cytotoxic potential against prostate (DU–145), ovarian (SKOV–3) and lung (A–549) cancer cell lines with cell viability of 14.25%, 16.02% and 27.24%, respectively. The IC50 of the total extract against HCT–116 and DU–145 cell lines were 7.81 μg/mL and 40.79 μg/mL, respectively. The observed cytotoxicity of the total methanolic extract from the leaves against colorectal cancer might facilitate future investigations on cytotoxic agent(s) for disease management.

Citovat

DEKINASH, Mohamed Fayez, Tarek M OKDA, Ehab Kotb ELMAHALLAWY, Fathy Kandil EL-FIKY, Gamal Abd El Hay OMRAN, Emil ŠVAJDLENKA, Naief DAHRAN, Manal F EL-KHADRAGY, Wafa A AL-MEGRIN a El Naggar El Moataz Bellah ALI. Insights into HPLC-MS/MS Analysis, Antioxidant and Cytotoxic Activity of Astragalus fruticosus against Different Types of Cancer Cell Lines. Pharmaceuticals. Basel: MDPI, 2022, roč. 15, č. 11, s. 1-16. ISSN 1424-8247. Dostupné z: https://dx.doi.org/10.3390/ph15111406.

@article{2280118,
   author = {Dekinash, Mohamed Fayez and Okda, Tarek M and Elmahallawy, Ehab Kotb and ElandFiky, Fathy Kandil and Omran, Gamal Abd El Hay and Švajdlenka, Emil and Dahran, Naief and ElandKhadragy, Manal F and AlandMegrin, Wafa A and Ali, El Naggar El Moataz Bellah},
   article_location = {Basel},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/ph15111406},
   keywords = {Astragalus fruticosus; HPLC-MS; MS profiling; colorectal cancer; cytotoxic activity},
   language = {eng},
   issn = {1424-8247},
   journal = {Pharmaceuticals},
   title = {Insights into HPLC-MS/MS Analysis, Antioxidant and Cytotoxic Activity of Astragalus fruticosus against Different Types of Cancer Cell Lines},
   url = {https://www.mdpi.com/1424-8247/15/11/1406},
   volume = {15},
   year = {2022}
}

TY  - JOUR
ID  - 2280118
AU  - Dekinash, Mohamed Fayez - Okda, Tarek M - Elmahallawy, Ehab Kotb - El-Fiky, Fathy Kandil - Omran, Gamal Abd El Hay - Švajdlenka, Emil - Dahran, Naief - El-Khadragy, Manal F - Al-Megrin, Wafa A - Ali, El Naggar El Moataz Bellah
PY  - 2022
TI  - Insights into HPLC-MS/MS Analysis, Antioxidant and Cytotoxic Activity of Astragalus fruticosus against Different Types of Cancer Cell Lines
JF  - Pharmaceuticals
VL  - 15
IS  - 11
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 14248247
KW  - Astragalus fruticosus
KW  - HPLC-MS
KW  - MS profiling
KW  - colorectal cancer
KW  - cytotoxic activity
UR  - https://www.mdpi.com/1424-8247/15/11/1406
N2  - Plants from the genus Astragalus are gaining attention for their pharmacological importance. However, the information available regarding the HPLC–MS/MS chemical profile of A. fruticosus is inadequate. In this study, we performed HPLC–MS/MS analysis using electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). We tentatively identified 11 compounds in the A. fruticosus methanolic extract, including five flavonoidal and six saponin glycosides. The extract showed moderate antioxidant activity with 21.05% reduction in DPPH UV absorption. The preliminary cytotoxic screening against seven human cancer cell lines using 100 μg/mL extract showed prominent cytotoxic potential against colorectal cancer HCT–116 with 3.368% cell viability. It also showed moderate cytotoxic potential against prostate (DU–145), ovarian (SKOV–3) and lung (A–549) cancer cell lines with cell viability of 14.25%, 16.02% and 27.24%, respectively. The IC50 of the total extract against HCT–116 and DU–145 cell lines were 7.81 μg/mL and 40.79 μg/mL, respectively. The observed cytotoxicity of the total methanolic extract from the leaves against colorectal cancer might facilitate future investigations on cytotoxic agent(s) for disease management.
ER  -

DEKINASH, Mohamed Fayez, Tarek M OKDA, Ehab Kotb ELMAHALLAWY, Fathy Kandil EL-FIKY, Gamal Abd El Hay OMRAN, Emil ŠVAJDLENKA, Naief DAHRAN, Manal F EL-KHADRAGY, Wafa A AL-MEGRIN a El Naggar El Moataz Bellah ALI. Insights into HPLC-MS/MS Analysis, Antioxidant and Cytotoxic Activity of Astragalus fruticosus against Different Types of Cancer Cell Lines. \textit{Pharmaceuticals}. Basel: MDPI, 2022, roč.~15, č.~11, s.~1-16. ISSN~1424-8247. Dostupné z: https://dx.doi.org/10.3390/ph15111406.

Podrobný výpis o publikaci