Escherichia coli from biopsies differ in virulence genes
between patients with colorectal neoplasia and healthy controls

J 2023

Escherichia coli from biopsies differ in virulence genes between patients with colorectal neoplasia and healthy controls

BOSÁK, Juraj; Darina KOHOUTOVÁ; Matěj HRALA; Jitka KŘENOVÁ; Paula MORÁVKOVÁ et al.

Základní údaje

Originální název

Escherichia coli from biopsies differ in virulence genes between patients with colorectal neoplasia and healthy controls

Autoři

BOSÁK, Juraj; Darina KOHOUTOVÁ; Matěj HRALA; Jitka KŘENOVÁ; Paula MORÁVKOVÁ; Stanislav REJCHRT; Jan BUREŠ a David ŠMAJS

Vydání

Frontiers in Microbiology, LAUSANNE, FRONTIERS MEDIA SA, 2023, 1664-302X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/23:00130804

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Escherichia coli; virulence factors; colorectal neoplasia; cancer; genotoxin; invasion; ibeA

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 2. 2024 10:34, Mgr. Tereza Miškechová

Anotace

V originále

Introduction: Pathogenic strains of Escherichia coli have been clearly identified as the causative agents of extraintestinal and diarrheal infections; however, the etiopathogenic role of E. coli in other conditions, including colorectal cancer, remains unclear. Methods: This study aimed to characterize mucosal E. coli isolates (n = 246) from 61 neoplasia patients and 20 healthy controls for the presence of 35 genetic determinants encoding known virulence factors. Results: Virulence determinants encoding invasin (ibeA), siderophore receptor (iroN), S-fimbriae (sfa), and genotoxin (usp) were more prevalent among E. coli isolated from patients with neoplasia compared to the control group (p < 0.05). In addition, the prevalence of these virulence determinants was increased in more advanced neoplasia stages (padj < 0.0125). Compared to patients with advanced colorectal adenoma and carcinoma, the ibeA gene was rarely found in the control group and among patients with non-advanced adenoma (p < 0.05), indicating its potential as the advanced-neoplasia biomarker. Patients with neoplasia frequently had E. coli strains with at least one of the abovementioned virulence factors, whereby specific combinations of these virulence factors were found. Discussion: These findings suggest that E. coli strains isolated from patients with colorectal neoplasia possess several virulence factors, which could contribute to the development of neoplastic processes in the large intestine.

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

MUNI/A/1393/2022, interní kód MU

Název: Biomedicínské vědy III

Investor: Masarykova univerzita, Biomedicínské vědy III

Přiložené soubory

Escherichia_coli_from_biopsies_differ_in_virulence_genes_between_patients_with_colorectal_neoplasia_and_healthy_controls.pdf

Citovat

BOSÁK, Juraj; Darina KOHOUTOVÁ; Matěj HRALA; Jitka KŘENOVÁ; Paula MORÁVKOVÁ; Stanislav REJCHRT; Jan BUREŠ a David ŠMAJS. Escherichia coli from biopsies differ in virulence genes between patients with colorectal neoplasia and healthy controls. Frontiers in Microbiology. LAUSANNE: FRONTIERS MEDIA SA, 2023, roč. 14, April 2023, s. 1-10. ISSN 1664-302X. Dostupné z: https://doi.org/10.3389/fmicb.2023.1141619.

@article{2283822,
   author = {Bosák, Juraj and Kohoutová, Darina and Hrala, Matěj and Křenová, Jitka and Morávková, Paula and Rejchrt, Stanislav and Bureš, Jan and Šmajs, David},
   article_location = {LAUSANNE},
   article_number = {April 2023},
   doi = {https://doi.org/10.3389/fmicb.2023.1141619},
   keywords = {Escherichia coli; virulence factors; colorectal neoplasia; cancer; genotoxin; invasion; ibeA},
   language = {eng},
   issn = {1664-302X},
   journal = {Frontiers in Microbiology},
   title = {Escherichia coli from biopsies differ in virulence genes between patients with colorectal neoplasia and healthy controls},
   url = {https://www.frontiersin.org/articles/10.3389/fmicb.2023.1141619/full},
   volume = {14},
   year = {2023}
}

TY  - JOUR
ID  - 2283822
AU  - Bosák, Juraj - Kohoutová, Darina - Hrala, Matěj - Křenová, Jitka - Morávková, Paula - Rejchrt, Stanislav - Bureš, Jan - Šmajs, David
PY  - 2023
TI  - Escherichia coli from biopsies differ in virulence genes between patients with colorectal neoplasia and healthy controls
JF  - Frontiers in Microbiology
VL  - 14
IS  - April 2023
SP  - 1-10
EP  - 1-10
PB  - FRONTIERS MEDIA SA
SN  - 1664302X
KW  - Escherichia coli
KW  - virulence factors
KW  - colorectal neoplasia
KW  - cancer
KW  - genotoxin
KW  - invasion
KW  - ibeA
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2023.1141619/full
N2  - Introduction: Pathogenic strains of Escherichia coli have been clearly identified as the causative agents of extraintestinal and diarrheal infections; however, the etiopathogenic role of E. coli in other conditions, including colorectal cancer, remains unclear. Methods: This study aimed to characterize mucosal E. coli isolates (n = 246) from 61 neoplasia patients and 20 healthy controls for the presence of 35 genetic determinants encoding known virulence factors. Results: Virulence determinants encoding invasin (ibeA), siderophore receptor (iroN), S-fimbriae (sfa), and genotoxin (usp) were more prevalent among E. coli isolated from patients with neoplasia compared to the control group (p < 0.05). In addition, the prevalence of these virulence determinants was increased in more advanced neoplasia stages (padj < 0.0125). Compared to patients with advanced colorectal adenoma and carcinoma, the ibeA gene was rarely found in the control group and among patients with non-advanced adenoma (p < 0.05), indicating its potential as the advanced-neoplasia biomarker. Patients with neoplasia frequently had E. coli strains with at least one of the abovementioned virulence factors, whereby specific combinations of these virulence factors were found. Discussion: These findings suggest that E. coli strains isolated from patients with colorectal neoplasia possess several virulence factors, which could contribute to the development of neoplastic processes in the large intestine.
ER  -

BOSÁK, Juraj; Darina KOHOUTOVÁ; Matěj HRALA; Jitka KŘENOVÁ; Paula MORÁVKOVÁ; Stanislav REJCHRT; Jan BUREŠ a David ŠMAJS. Escherichia coli from biopsies differ in virulence genes between patients with colorectal neoplasia and healthy controls. \textit{Frontiers in Microbiology}. LAUSANNE: FRONTIERS MEDIA SA, 2023, roč.~14, April 2023, s.~1-10. ISSN~1664-302X. Dostupné z: https://doi.org/10.3389/fmicb.2023.1141619.

Přehled o publikaci