Interfacial behaviour of oligodeoxynucleotides prone to
G-quadruplex formation on negatively charged electrode surface
monitored by electrochemical probes

J 2023

Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes

DOBROVODSKÝ, Daniel; Aleš DAŇHEL; Emmanuelle MOTHES-MARTIN; Genevieve PRATVIEL; Daniel RENČIUK et al.

Základní údaje

Originální název

Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes

Autoři

DOBROVODSKÝ, Daniel; Aleš DAŇHEL; Emmanuelle MOTHES-MARTIN; Genevieve PRATVIEL; Daniel RENČIUK; Jean-Louis MERGNY a Miroslav FOJTA

Vydání

Electrochimica Acta, Elsevier, 2023, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.500

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00130873

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

DNA; Adsorption; G-quadruplex; Porphyrin; Voltammetry

Štítky

143160, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 6. 2023 14:52, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Development of analytical tools enabling discrimination amongst various structures of nucleic acids attracts interest due to diverse biological functions and prospective biotechnological applications of the latter. In this work, two electrochemically active compounds, copper porphyrin complex Cu-TMPy2PP and base-modified deoxyguanosine triphosphate conjugate bearing 3-nitrophenyl moiety (dGNPTP), were used as probes to study electrode potential effects on oligodeoxynucleotides (ODNs) of various sequences and abilities to form secondary structures (including unstructured single strands, duplexes, guanine quadruplexes, i-motifs) at the surface of hanging mercury drop electrode (HMDE). Our experiments revealed formation of less dense adsorbed layers by G-rich ODNs with the propensity to fold into G-quadruplexes (G4), compared to layers formed by other studied sequences. Strikingly, the G4-forming ODNs were significantly more susceptible to desorption from the HMDE surface during its polarization to highly negative potentials compared to other ODNs. Negative potential-induced desorption of the ODNs from HMDE was further confirmed by gel electrophoresis. Application of the electro-chemical probes offers a useful method for further research of DNA conformations and transitions taking place in solution and at electrically charged surfaces.

Citovat

DOBROVODSKÝ, Daniel; Aleš DAŇHEL; Emmanuelle MOTHES-MARTIN; Genevieve PRATVIEL; Daniel RENČIUK; Jean-Louis MERGNY a Miroslav FOJTA. Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes. Electrochimica Acta. Elsevier, 2023, roč. 442, February, s. 1-8. ISSN 0013-4686. Dostupné z: https://doi.org/10.1016/j.electacta.2023.141878.

@article{2287057,
   author = {Dobrovodský, Daniel and Daňhel, Aleš and MothesandMartin, Emmanuelle and Pratviel, Genevieve and Renčiuk, Daniel and Mergny, JeanandLouis and Fojta, Miroslav},
   article_number = {February},
   doi = {https://doi.org/10.1016/j.electacta.2023.141878},
   keywords = {DNA; Adsorption; G-quadruplex; Porphyrin; Voltammetry},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes},
   url = {https://doi.org/10.1016/j.electacta.2023.141878},
   volume = {442},
   year = {2023}
}

TY  - JOUR
ID  - 2287057
AU  - Dobrovodský, Daniel - Daňhel, Aleš - Mothes-Martin, Emmanuelle - Pratviel, Genevieve - Renčiuk, Daniel - Mergny, Jean-Louis - Fojta, Miroslav
PY  - 2023
TI  - Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes
JF  - Electrochimica Acta
VL  - 442
IS  - February
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 00134686
KW  - DNA
KW  - Adsorption
KW  - G-quadruplex
KW  - Porphyrin
KW  - Voltammetry
UR  - https://doi.org/10.1016/j.electacta.2023.141878
N2  - Development of analytical tools enabling discrimination amongst various structures of nucleic acids attracts interest due to diverse biological functions and prospective biotechnological applications of the latter. In this work, two electrochemically active compounds, copper porphyrin complex Cu-TMPy2PP and base-modified deoxyguanosine triphosphate conjugate bearing 3-nitrophenyl moiety (dGNPTP), were used as probes to study electrode potential effects on oligodeoxynucleotides (ODNs) of various sequences and abilities to form secondary structures (including unstructured single strands, duplexes, guanine quadruplexes, i-motifs) at the surface of hanging mercury drop electrode (HMDE). Our experiments revealed formation of less dense adsorbed layers by G-rich ODNs with the propensity to fold into G-quadruplexes (G4), compared to layers formed by other studied sequences. Strikingly, the G4-forming ODNs were significantly more susceptible to desorption from the HMDE surface during its polarization to highly negative potentials compared to other ODNs. Negative potential-induced desorption of the ODNs from HMDE was further confirmed by gel electrophoresis. Application of the electro-chemical probes offers a useful method for further research of DNA conformations and transitions taking place in solution and at electrically charged surfaces.
ER  -

DOBROVODSKÝ, Daniel; Aleš DAŇHEL; Emmanuelle MOTHES-MARTIN; Genevieve PRATVIEL; Daniel RENČIUK; Jean-Louis MERGNY a Miroslav FOJTA. Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes. \textit{Electrochimica Acta}. Elsevier, 2023, roč.~442, February, s.~1-8. ISSN~0013-4686. Dostupné z: https://doi.org/10.1016/j.electacta.2023.141878.

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