Proteases and their inhibitors involved in Schistosoma mansoni
egg-host interaction revealed by comparative transcriptomics
with Fasciola hepatica eggs

J 2023

Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs

PETERKOVÁ, Kristýna, Jiří VOREL, Jana ILGOVÁ, Pavel OSTAŠOV, Pavla FAJTOVÁ et. al.

Základní údaje

Originální název

Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs

Autoři

PETERKOVÁ, Kristýna (203 Česká republika, garant), Jiří VOREL (203 Česká republika, domácí), Jana ILGOVÁ (703 Slovensko, domácí), Pavel OSTAŠOV, Pavla FAJTOVÁ, Lukáš KONEČNÝ, Marta CHÁNOVA, Martin KAŠNÝ (203 Česká republika, domácí), Martin HORN a Jan DVOŘÁK

Vydání

International Journal for Parasitology, Oxford, Elsevier Science Ltd. 2023, 0020-7519

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.000 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131129

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.ijpara.2022.12.007

UT WoS

001002409800001

Klíčová slova anglicky

Schistosoma mansoni; Egg; Fasciola hepatica; Comparative transcriptomics; Protease; Protease inhibitor

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 11. 2023 10:55, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

Schistosoma mansoni eggs are the main causative agents of the pathological manifestations of schistoso-miasis. The eggs are laid in the host bloodstream, then they migrate through the intestinal wall into the lumen. However, a significant proportion of the eggs become lodged in the liver, where they cause inflammation and fibrosis. In this study, we focus on a specific group of proteins expressed by the egg, namely proteases and their inhibitors. These molecules are often involved in schistosome-host interac-tions, but are still unexplored in the egg stage. Using RNA-seq and comparative transcriptomics of imma-ture and mature S. mansoni eggs, we mapped the portfolio of proteases and their inhibitors, and determined their gene expression levels. In addition, we compared these data with gene expression of proteases and their inhibitors in Fasciola hepatica eggs. Fasciola hepatica eggs served as a useful compar-ative model, as they do not migrate through tissues and inflict pathology. We detected transcription of 135 and 117 proteases in S. mansoni and F. hepatica eggs, respectively, with 87 identified as orthologous between the two species. In contrast, we observed only four orthologous inhibitors out of 21 and 16 iden-tified in S. mansoni and F. hepatica eggs, respectively. Among others, we measured high and developmen-tally regulated levels of expression of metalloproteases in S. mansoni eggs, specifically aminopeptidase N1, endothelin-converting enzyme 1, and several leishmanolysin-like peptidases. We identified highly transcribed protease inhibitors serpin and alpha-2-macroglobulin that are unique to S. mansoni eggs, and antistasin-like inhibitor in F. hepatica eggs. This study provides new insights into the portfolio of pro -teases and inhibitors expressed by S. mansoni with potential roles in egg tissue migration, stimulation of angiogenesis, and interaction with host blood and immunity.

Návaznosti

GA19-17269S, projekt VaV

Název: Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace (Akronym: Proteolýza vajec červů)

Investor: Grantová agentura ČR, Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace

LM2018131, projekt VaV

Název: Česká národní infrastruktura pro biologická data (Akronym: ELIXIR-CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Česká národní infrastruktura pro biologická data

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

Citovat

PETERKOVÁ, Kristýna, Jiří VOREL, Jana ILGOVÁ, Pavel OSTAŠOV, Pavla FAJTOVÁ, Lukáš KONEČNÝ, Marta CHÁNOVA, Martin KAŠNÝ, Martin HORN a Jan DVOŘÁK. Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs. International Journal for Parasitology. Oxford: Elsevier Science Ltd., 2023, roč. 53, 5-6, s. 253-263. ISSN 0020-7519. Dostupné z: https://dx.doi.org/10.1016/j.ijpara.2022.12.007.

@article{2295018,
   author = {Peterková, Kristýna and Vorel, Jiří and Ilgová, Jana and Ostašov, Pavel and Fajtová, Pavla and Konečný, Lukáš and Chánova, Marta and Kašný, Martin and Horn, Martin and Dvořák, Jan},
   article_location = {Oxford},
   article_number = {5-6},
   doi = {http://dx.doi.org/10.1016/j.ijpara.2022.12.007},
   keywords = {Schistosoma mansoni; Egg; Fasciola hepatica; Comparative transcriptomics; Protease; Protease inhibitor},
   language = {eng},
   issn = {0020-7519},
   journal = {International Journal for Parasitology},
   title = {Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs},
   url = {https://doi.org/10.1016/j.ijpara.2022.12.007},
   volume = {53},
   year = {2023}
}

TY  - JOUR
ID  - 2295018
AU  - Peterková, Kristýna - Vorel, Jiří - Ilgová, Jana - Ostašov, Pavel - Fajtová, Pavla - Konečný, Lukáš - Chánova, Marta - Kašný, Martin - Horn, Martin - Dvořák, Jan
PY  - 2023
TI  - Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs
JF  - International Journal for Parasitology
VL  - 53
IS  - 5-6
SP  - 253-263
EP  - 253-263
PB  - Elsevier Science Ltd.
SN  - 00207519
KW  - Schistosoma mansoni
KW  - Egg
KW  - Fasciola hepatica
KW  - Comparative transcriptomics
KW  - Protease
KW  - Protease inhibitor
UR  - https://doi.org/10.1016/j.ijpara.2022.12.007
N2  - Schistosoma mansoni eggs are the main causative agents of the pathological manifestations of schistoso-miasis. The eggs are laid in the host bloodstream, then they migrate through the intestinal wall into the lumen. However, a significant proportion of the eggs become lodged in the liver, where they cause inflammation and fibrosis. In this study, we focus on a specific group of proteins expressed by the egg, namely proteases and their inhibitors. These molecules are often involved in schistosome-host interac-tions, but are still unexplored in the egg stage. Using RNA-seq and comparative transcriptomics of imma-ture and mature S. mansoni eggs, we mapped the portfolio of proteases and their inhibitors, and determined their gene expression levels. In addition, we compared these data with gene expression of proteases and their inhibitors in Fasciola hepatica eggs. Fasciola hepatica eggs served as a useful compar-ative model, as they do not migrate through tissues and inflict pathology. We detected transcription of 135 and 117 proteases in S. mansoni and F. hepatica eggs, respectively, with 87 identified as orthologous between the two species. In contrast, we observed only four orthologous inhibitors out of 21 and 16 iden-tified in S. mansoni and F. hepatica eggs, respectively. Among others, we measured high and developmen-tally regulated levels of expression of metalloproteases in S. mansoni eggs, specifically aminopeptidase N1, endothelin-converting enzyme 1, and several leishmanolysin-like peptidases. We identified highly transcribed protease inhibitors serpin and alpha-2-macroglobulin that are unique to S. mansoni eggs, and antistasin-like inhibitor in F. hepatica eggs. This study provides new insights into the portfolio of pro -teases and inhibitors expressed by S. mansoni with potential roles in egg tissue migration, stimulation of angiogenesis, and interaction with host blood and immunity.
ER  -

PETERKOVÁ, Kristýna, Jiří VOREL, Jana ILGOVÁ, Pavel OSTAŠOV, Pavla FAJTOVÁ, Lukáš KONEČNÝ, Marta CHÁNOVA, Martin KAŠNÝ, Martin HORN a Jan DVOŘÁK. Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs. \textit{International Journal for Parasitology}. Oxford: Elsevier Science Ltd., 2023, roč.~53, 5-6, s.~253-263. ISSN~0020-7519. Dostupné z: https://dx.doi.org/10.1016/j.ijpara.2022.12.007.

Podrobný výpis o publikaci