Transcriptomic responses of bat cells to European bat
lyssavirus 1 infection under conditions simulating euthermia
and hibernation

J 2023

Transcriptomic responses of bat cells to European bat lyssavirus 1 infection under conditions simulating euthermia and hibernation

HARAZIM, Markéta; Juliette PERROT; Hugo VARET; Herve BOURHY; Julien LANNOY et al.

Základní údaje

Originální název

Transcriptomic responses of bat cells to European bat lyssavirus 1 infection under conditions simulating euthermia and hibernation

Autoři

HARAZIM, Markéta; Juliette PERROT; Hugo VARET; Herve BOURHY; Julien LANNOY; Jiri PIKULA; Veronika SEIDLOVA; Laurent DACHEUX a Natália MARTÍNKOVÁ

Vydání

BMC immunology, BMC, 2023, 1471-2172

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00131151

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Chiroptera; Myotis myotis; Hibernation; Lyssaviruses; In vitro infection model; EBLV-1; Innate immunity; Heat shock proteins (HSPs); Antiviral state; Transcriptome

Štítky

14314020, 143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 3. 2024 13:01, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Background Coevolution between pathogens and their hosts decreases host morbidity and mortality. Bats host and can tolerate viruses which can be lethal to other vertebrate orders, including humans. Bat adaptations to infection include localized immune response, early pathogen sensing, high interferon expression without pathogen stimulation, and regulated inflammatory response. The immune reaction is costly, and bats suppress high-cost metabolism during torpor. In the temperate zone, bats hibernate in winter, utilizing a specific behavioural adaptation to survive detrimental environmental conditions and lack of energy resources. Hibernation torpor involves major physiological changes that pose an additional challenge to bat-pathogen coexistence. Here, we compared bat cellular reaction to viral challenge under conditions simulating hibernation, evaluating the changes between torpor and euthermia. Results We infected the olfactory nerve-derived cell culture of Myotis myotis with an endemic bat pathogen, European bat lyssavirus 1 (EBLV-1). After infection, the bat cells were cultivated at two different temperatures, 37 degrees C and 5 degrees C, to examine the cell response during conditions simulating euthermia and torpor, respectively. The mRNA isolated from the cells was sequenced and analysed for differential gene expression attributable to the temperature and/or infection treatment. In conditions simulating euthermia, infected bat cells produce an excess signalling by multitude of pathways involved in apoptosis and immune regulation influencing proliferation of regulatory cell types which can, in synergy with other produced cytokines, contribute to viral tolerance. We found no up- or down-regulated genes expressed in infected cells cultivated at conditions simulating torpor compared to non-infected cells cultivated under the same conditions. When studying the reaction of uninfected cells to the temperature treatment, bat cells show an increased production of heat shock proteins (HSPs) with chaperone activity, improving the bat's ability to repair molecular structures damaged due to the stress related to the temperature change. Conclusions The lack of bat cell reaction to infection in conditions simulating hibernation may contribute to the virus tolerance or persistence in bats. Together with the cell damage repair mechanisms induced in response to hibernation, the immune regulation may promote bats' ability to act as reservoirs of zoonotic viruses such as lyssaviruses.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

MUNI/A/1098/2019, interní kód MU

Název: Výzkum Ekologických a Evolučních Principů na modelu obratlovců a jejich parazitů

Investor: Masarykova univerzita, Výzkum Ekologických a Evolučních Principů na modelu obratlovců a jejich parazitů, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Citovat

HARAZIM, Markéta; Juliette PERROT; Hugo VARET; Herve BOURHY; Julien LANNOY; Jiri PIKULA; Veronika SEIDLOVA; Laurent DACHEUX a Natália MARTÍNKOVÁ. Transcriptomic responses of bat cells to European bat lyssavirus 1 infection under conditions simulating euthermia and hibernation. BMC immunology. BMC, 2023, roč. 24, č. 1, s. 1-13. ISSN 1471-2172. Dostupné z: https://doi.org/10.1186/s12865-023-00542-7.

@article{2295487,
   author = {Harazim, Markéta and Perrot, Juliette and Varet, Hugo and Bourhy, Herve and Lannoy, Julien and Pikula, Jiri and Seidlova, Veronika and Dacheux, Laurent and Martínková, Natália},
   article_number = {1},
   doi = {https://doi.org/10.1186/s12865-023-00542-7},
   keywords = {Chiroptera; Myotis myotis; Hibernation; Lyssaviruses; In vitro infection model; EBLV-1; Innate immunity; Heat shock proteins (HSPs); Antiviral state; Transcriptome},
   language = {eng},
   issn = {1471-2172},
   journal = {BMC immunology},
   title = {Transcriptomic responses of bat cells to European bat lyssavirus 1 infection under conditions simulating euthermia and hibernation},
   url = {https://doi.org/10.1186/s12865-023-00542-7},
   volume = {24},
   year = {2023}
}

TY  - JOUR
ID  - 2295487
AU  - Harazim, Markéta - Perrot, Juliette - Varet, Hugo - Bourhy, Herve - Lannoy, Julien - Pikula, Jiri - Seidlova, Veronika - Dacheux, Laurent - Martínková, Natália
PY  - 2023
TI  - Transcriptomic responses of bat cells to European bat lyssavirus 1 infection under conditions simulating euthermia and hibernation
JF  - BMC immunology
VL  - 24
IS  - 1
SP  - 1-13
EP  - 1-13
PB  - BMC
SN  - 14712172
KW  - Chiroptera
KW  - Myotis myotis
KW  - Hibernation
KW  - Lyssaviruses
KW  - In vitro infection model
KW  - EBLV-1
KW  - Innate immunity
KW  - Heat shock proteins (HSPs)
KW  - Antiviral state
KW  - Transcriptome
UR  - https://doi.org/10.1186/s12865-023-00542-7
N2  - Background Coevolution between pathogens and their hosts decreases host morbidity and mortality. Bats host and can tolerate viruses which can be lethal to other vertebrate orders, including humans. Bat adaptations to infection include localized immune response, early pathogen sensing, high interferon expression without pathogen stimulation, and regulated inflammatory response. The immune reaction is costly, and bats suppress high-cost metabolism during torpor. In the temperate zone, bats hibernate in winter, utilizing a specific behavioural adaptation to survive detrimental environmental conditions and lack of energy resources. Hibernation torpor involves major physiological changes that pose an additional challenge to bat-pathogen coexistence. Here, we compared bat cellular reaction to viral challenge under conditions simulating hibernation, evaluating the changes between torpor and euthermia. Results We infected the olfactory nerve-derived cell culture of Myotis myotis with an endemic bat pathogen, European bat lyssavirus 1 (EBLV-1). After infection, the bat cells were cultivated at two different temperatures, 37 degrees C and 5 degrees C, to examine the cell response during conditions simulating euthermia and torpor, respectively. The mRNA isolated from the cells was sequenced and analysed for differential gene expression attributable to the temperature and/or infection treatment. In conditions simulating euthermia, infected bat cells produce an excess signalling by multitude of pathways involved in apoptosis and immune regulation influencing proliferation of regulatory cell types which can, in synergy with other produced cytokines, contribute to viral tolerance. We found no up- or down-regulated genes expressed in infected cells cultivated at conditions simulating torpor compared to non-infected cells cultivated under the same conditions. When studying the reaction of uninfected cells to the temperature treatment, bat cells show an increased production of heat shock proteins (HSPs) with chaperone activity, improving the bat's ability to repair molecular structures damaged due to the stress related to the temperature change. Conclusions The lack of bat cell reaction to infection in conditions simulating hibernation may contribute to the virus tolerance or persistence in bats. Together with the cell damage repair mechanisms induced in response to hibernation, the immune regulation may promote bats' ability to act as reservoirs of zoonotic viruses such as lyssaviruses.
ER  -

HARAZIM, Markéta; Juliette PERROT; Hugo VARET; Herve BOURHY; Julien LANNOY; Jiri PIKULA; Veronika SEIDLOVA; Laurent DACHEUX a Natália MARTÍNKOVÁ. Transcriptomic responses of bat cells to European bat lyssavirus 1 infection under conditions simulating euthermia and hibernation. \textit{BMC immunology}. BMC, 2023, roč.~24, č.~1, s.~1-13. ISSN~1471-2172. Dostupné z: https://doi.org/10.1186/s12865-023-00542-7.

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