Development of recombinant West Nile virus expressing mCherry
reporter protein.

J 2023

Development of recombinant West Nile virus expressing mCherry reporter protein.

KOBAYASHI, Shintaro; Yukine FUKUDA; Kentaro YOSHII; Passawat THAMMAHAKIN; Keisuke MAEZONO et al.

Základní údaje

Originální název

Development of recombinant West Nile virus expressing mCherry reporter protein.

Autoři

KOBAYASHI, Shintaro; Yukine FUKUDA; Kentaro YOSHII; Passawat THAMMAHAKIN; Keisuke MAEZONO; Luděk EYER; Daniel RŮŽEK a Hiroaki KARIWA

Vydání

Journal of Virological Methods, Amsterdam, Elsevier, 2023, 0166-0934

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00131190

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

West Nile virus; Reporter protein; Production of reporter virus

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 9. 2023 10:32, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

West Nile virus (WNV) is transmitted to humans and animals by a mosquito and enters the central nervous system, leading to lethal encephalitis. Reporter viruses expressing fluorescent proteins enable detection of infected cells in vitro and in vivo, facilitating evaluation of the dynamics of viral infection, and the development of diagnostic or therapeutic methods. In this study, we developed a method for production of a recombinant replication-competent WNV expressing mCherry fluorescent protein. The expression of mCherry was observed in viral antigen-positive cells in vitro and in vivo, but the growth of the reporter WNV was reduced as compared to the parental WNV. The expression of mCherry was stable during 5 passages in reporter WNV-infected culture cells. Neurological symptoms were observed in mice inoculated intracranially with the reporter WNV. The reporter WNV expressing mCherry will facilitate research into WNV replication in mouse brains.

Citovat

KOBAYASHI, Shintaro; Yukine FUKUDA; Kentaro YOSHII; Passawat THAMMAHAKIN; Keisuke MAEZONO; Luděk EYER; Daniel RŮŽEK a Hiroaki KARIWA. Development of recombinant West Nile virus expressing mCherry reporter protein. Journal of Virological Methods. Amsterdam: Elsevier, 2023, roč. 317, July, s. 1-5. ISSN 0166-0934. Dostupné z: https://doi.org/10.1016/j.jviromet.2023.114744.

@article{2296941,
   author = {Kobayashi, Shintaro and Fukuda, Yukine and Yoshii, Kentaro and Thammahakin, Passawat and Maezono, Keisuke and Eyer, Luděk and Růžek, Daniel and Kariwa, Hiroaki},
   article_location = {Amsterdam},
   article_number = {July},
   doi = {https://doi.org/10.1016/j.jviromet.2023.114744},
   keywords = {West Nile virus; Reporter protein; Production of reporter virus},
   language = {eng},
   issn = {0166-0934},
   journal = {Journal of Virological Methods},
   title = {Development of recombinant West Nile virus expressing mCherry reporter protein.},
   url = {https://doi.org/10.1016/j.jviromet.2023.114744},
   volume = {317},
   year = {2023}
}

TY  - JOUR
ID  - 2296941
AU  - Kobayashi, Shintaro - Fukuda, Yukine - Yoshii, Kentaro - Thammahakin, Passawat - Maezono, Keisuke - Eyer, Luděk - Růžek, Daniel - Kariwa, Hiroaki
PY  - 2023
TI  - Development of recombinant West Nile virus expressing mCherry reporter protein.
JF  - Journal of Virological Methods
VL  - 317
IS  - July
SP  - 1-5
EP  - 1-5
PB  - Elsevier
SN  - 01660934
KW  - West Nile virus
KW  - Reporter protein
KW  - Production of reporter virus
UR  - https://doi.org/10.1016/j.jviromet.2023.114744
N2  - West Nile virus (WNV) is transmitted to humans and animals by a mosquito and enters the central nervous system, leading to lethal encephalitis. Reporter viruses expressing fluorescent proteins enable detection of infected cells in vitro and in vivo, facilitating evaluation of the dynamics of viral infection, and the development of diagnostic or therapeutic methods. In this study, we developed a method for production of a recombinant replication-competent WNV expressing mCherry fluorescent protein. The expression of mCherry was observed in viral antigen-positive cells in vitro and in vivo, but the growth of the reporter WNV was reduced as compared to the parental WNV. The expression of mCherry was stable during 5 passages in reporter WNV-infected culture cells. Neurological symptoms were observed in mice inoculated intracranially with the reporter WNV. The reporter WNV expressing mCherry will facilitate research into WNV replication in mouse brains.
ER  -

KOBAYASHI, Shintaro; Yukine FUKUDA; Kentaro YOSHII; Passawat THAMMAHAKIN; Keisuke MAEZONO; Luděk EYER; Daniel RŮŽEK a Hiroaki KARIWA. Development of recombinant West Nile virus expressing mCherry reporter protein. \textit{Journal of Virological Methods}. Amsterdam: Elsevier, 2023, roč.~317, July, s.~1-5. ISSN~0166-0934. Dostupné z: https://doi.org/10.1016/j.jviromet.2023.114744.

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